Left: Past and present members of the Stanley lab, USDA, ARS, BCIRL, Columbia, MO (from left to right): Yong Wang, Yuping Huang, Aaron Lewis, Christine Sapp, Tamra Reall, Hongwei Zhang, Joseph Ringbauer, Jr., David Stanley, Yaoyu Bai, Stephen Saathoff, Claire Dubos, Cynthia Goodman (authors are in bold). Right: Members of the Geibel lab, Bayer AG, Monheim am Rhein, Germany (from left top to bottom right: Corinna Saalwaechter, Sven Geibel, Konstantin Herppich, Susanne Dohn (formerly Susanne Kraus), Beate Brueck, Susanne Lohmann, Kirsten Backhaus, Patricia Hoeschler (authors are in bold).
The fall armyworm, Spodoptera frugiperda (Sf), is a polyphagous lepidopteran herbivore that consumes more than 80 plant species, including many economically important crops, such as corn, soybeans, and sorghum. It is a serious pest in most of North and South America and has spread to India, China and other Asian countries, as well as to Africa. Sf was recently categorized as a potential European Union quarantine pest because its range may expand from Africa into Europe. There are effective barriers to Sf management, such as rapid evolution of resistance to chemical pesticides, immune responses to biological control agents, and field-evolved resistance to Cry proteins. New Sf management technologies are necessary. We are working to reduce development times to invent new insect control technologies by creating novel, next-generation Sf cell lines. By ‘next-generation’, we refer to cell lines established from specific tissues or from species that have proven to be difficult sources for generating cell lines. Insect cell lines have strong potential as research platforms and are used in many ways, including in insecticide discovery programs. Our laboratory has a lengthy history of establishing insect cell lines, applying them to important research issues and providing them to researchers in universities, governments and industries. Here, we report on the establishment of 16 new cell lines from three Sf tissues: nine from the central nervous system, three from aorta, and four from testes. We created 16 sub-lines from three neuronal cell lines. We confirmed the identities of the cell lines by DAF-PCR, determined their doubling times from growth curves, and described cell types via microscopy. All cell lines have been passaged at least 25 times, all sub-lines have been passaged at least 10 times, and are now established.
Tamra Reall, Susanne Kraus, Cynthia L. Goodman, Joseph Ringbauer Jr., Sven Geibel and David Stanley. Next-generation Cell Lines Established from the Fall Armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae). In Vitro Cellular & Developmental Biology – Animal 55:686–693, 2019.