The Distinguished Service Award is presented to those, selected by the SIVB President, who have demonstrated and given extra effort in support of the SIVB programs and endeavors. The following SIVB members were presented the Distinguished Service Award at the 2004 World Congress, held in San Francisco, California. Additional winners will be listed in future issues of the In Vitro Report.
In September 2004, Barbara Doonan celebrated 25 years as a scientist in industry. Her current position is Lead Senior Scientist at UST Inc. in Greenwich CT. At UST since 1989, she has enjoyed the opportunity to apply her broad background and expertise in biochemistry, molecular biology and toxicology to address company interests in areas such as plant biotechnology and health issues. Just prior to joining UST, Dr. Doonan was a visiting scientist at the Cold Spring Harbor laboratory of Dr. Elizabeth Moran researching cell cycle control in the adenovirus E1A system utilizing recombinant technologies. Her career in industry was initiated in 1979 at General Foods Corporation (GF) as a Research Scientist in the Nutrition and Health division directing such as short term animal toxicology GLP studies and studies designed to determine carcinogenic and mutagenic potential of various compounds e.g. halogenated hydrocarbons. Many of these projects were conducted in support of GF’s domestic and international coffee businesses addressing questions as to its safety aspects. This provided an excellent in depth introduction to this crop for Dr. Doonan which was enhanced further as she transitioned into GF’s Biotechnology Programs group to assume the direction of studies utilizing cell culture and microbial fermentation technologies for production of natural compounds e.g. specific flavors/unique enzymes from coffee precursors. In addition to teaching duties while a graduate student, research at the Master’s level focused on the study of bacteria and their viruses via development of a novel transformation system. Building on this foundation, she further expanded her background concentrating on the biosynthesis and metabolism of phosphate compounds in cyanobacteria and the development of improved techniques for visualization of enzyme activities at the molecular level. After receiving her doctoral degree from City University of New York, Dr. Doonan spent a year as an assistant professor at William Paterson College in Wayne NJ teaching general biology, human anatomy and physiology and electron microscopy. She is a member of Phi Beta Kappa, Sigma Xi, the Society of In Vitro Biology and a number of other professional societies.
Gertrude C. Buehring
My professional career began at the University of California at Berkeley 31 years ago when I was hired on the faculty of the Infectious Division of the School of Public Health. I have been there ever since. I teach two undergraduate courses, Public Health Microbiology, lecture and laboratory component. I teach one graduate course called “Viruses and Human Cancer” and occasionally offer special seminar courses. Throughout my time on the faculty here, I have maintained a research program on human breast cancer and have mentored in my laboratory over 20 Ph.D. students. Initially I worked on developing methods to grow human mammary epithelial cells in culture and study their characteristics, e.g. morphology, antigen expression, hormone responsiveness. For the past 15 years I have focused on exploring the possibility that bovine leukemia virus (BLV) might play a causal role in the development of human breast cancer. Our laboratory has been funded for this research for the past 8 years and has been able to unravel some important information about the basic biology of BLV, as well as to study its ability to infect humans and its association with breast cancer. Cell culture has been a methodology important in my research and I have valued my membership in SIVB (since 1978) and attendance at meetings as an important way to keep up with advances in the field. I have particularly enjoyed the friendships and collegial relationships I have developed with other scientists in SIVB.
Glyn Stacey’s early scientific background was been in microbiology and cancer research. He has established special interests and activities in the field of cell biology relating to genetic stability, safety issues and standardization. This has also lead to involvement in a range of related areas including plant tissue culture, cell preservation and management of biological resources. From 1989-1998 he worked at the Centre for Applied Biology and Research where he was involved in developing cell culture and cell banking procedures and in establishing a cell biology unit working on the development of cell substrates for manufacture of medical products and cell-based diagnostic assays. Part of Dr Stacey’s initial work at NIBSC was focused on creating a new suite of clean-room laboratories designed to operate under GMP accreditation for cell banking. At NIBSC he has developed a broad remit on the development appropriate cell biology activities relevant to the quality and safety of new biological medicines and therapies based on the use of human and animal cells. In addition to developing a research portfolio in this area, he has also acted as an advisor to the UK Department of Health and the World Health Organization and was a member of the consultation group that drafted the UK Code of Practice for the Production of Human-derived Therapeutic Products (June 2002). He has organized the NIBSC Stem Cell Liaison Group which provides an open forum for discussion amongst a variety of health care workers on safety and quality issues in the preparation of harvested haematopoietic stem cells for transplantation. He is now in charge of the UK Stem Cell Bank funded by the Medical Research Council and BBSRC to prepare banks of stem cell lines for use in research and in the development of new therapies and in addition is a visiting Professor on a new international postgraduate course in Cryobiology at the University of Luton.
The major interest of our laboratory is to understand the differentiation-dependent replication of papillomaviruses and their role in carcinogenesis. Human papillomaviruses (HPV) are important human pathogens associated with benign and malignant neoplasias. HPV is the major risk factor in the development of cervical cancer. A major obstacle was once the inability to study the HPV life cycle in vitro. Using an organotypic (raft) culture system, my laboratory demonstrated for the first time the complete HPV life cycle in vitro, concomitant with the induction of a more complete differentiation program of the host tissue. The organotypic raft culture system faithfully mimics three-dimensional epithelial physiology in an in vitro model system. Using this in vitro organotypic culture system we are analyzing the steps and mechanisms (viral and cellular) involved in viral DNA replication, gene expression, and oncogenesis. We are identifying and investigating the role of cofactors, such as other viruses and hormones, in the carcinogenic process. We have also developed numerous genetic systems to study the natural history of HPV infection in vitro. Additionally, we are using our system to support vaccine and therapeutic drug studies.