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11th International Conference on Invertebrate Cell And Tissue Culuture
11th International Conference on Invertebrate Cell and Tissue
Culture
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Held
in conjunction with the 2004 World Congress on In Vitro
Biology
May 22 - 26, 2004
San Francisco, California
Corporate Donors
CSIRO
Entomology
Promega Corporation
Association Donors
Chair: Amy A. Wang, GlaxoSmithKline
Co-Chair: Guy Smagghe, PhD, Ghent University
Invertebrate Program Committee
Cynthia Goodman, PhD, USDA, ARS, BCIRL
Robert R. Granados, PhD, Boyce Thompson Institute for
Plant Research
Raziel S. Hakim, PhD, Howard University
J. Denry Sato, PhD, Mount Desert Biological Laboratory
Dwight Lynn, PhD, USDA
Marcia Loeb, PhD, USDA
Jun Mitsuhashi, PhD, Tokyo University Agriculture
Karl Maramorosch, PhD, Rutgers University
K. J. Magaratha Vally, PhD, Texas A&M University
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Molecular Engineering
and Biology of Invertebrate Cell Cultures: A Tribute to
Dr. Thomas Grace and Professor Shangyin Gao
Saturday,
May 22
8:30 am – 5:30 pm
Keynote and Opening Symposium
Conveners: Robert R. Granados, PhD, Boyce Thompson
Institute, Professor Karl Maramorosch, PhD, Rutgers
University, and Amy Wang, GlaxoSmithKline
In this symposium we are paying tribute to Dr. T. D. C.
Grace and Prof. Shangyin Gao (Z-Y.Gaw) to recognize their
specific tangible achievements in the field of invertebrate
cell culture. The exceptional and unique achievements of
these two pioneers have resulted in the rapid development
and important applications of insect cell culture during
the past four decades. Modern invertebrate cell culture
got started independently by the two scientists. Prof. Shangyin
Gao in Wuhan, China in 1958 and Tom Grace in Canberra, Australia
in 1962. The two have never met and they were unaware of
each other’s work, but they shared outstanding talents
as creative inventors. They have made invaluable contributions
to furthering the progress of the field and provided guidelines
for us to follow in the footsteps of these in vitro biotechnology
pioneers. The breakthrough achieved by Grace and Gao has
influenced virtually all subsequent research dealing with
insect cell culture. Therefore we are paying tribute to
these pioneers, recognizing that without their contributions
no achievement in modern molecular invertebrate cell culture
would have been possible. Several biotechnological advances
have driven the remarkable growth and application of insect
cell culture research during the past two decades. The emergence
of the baculovirus-insect cell culture system resulted from
intensive and elegant studies on the molecular biology of
baculoviruses and the development of novel insect virus-cell
culture systems. The use of in vitro expression systems
have not only become important tools for basic research
around the world, but represent a widely used technology
for the commercialization of products for use in agriculture
and human health. The speakers will represent some of the
leading authorities in areas relating to cell culture systems
and they represent the diversity of research from around
the world.
| 8:30 am |
Welcome and Announcements
Amy Wang, GlaxoSmithKline |
| 8:40 am |
Introduction and Seminal
Research Contributions by T. D. Grace and S. Gao
Karl Maramorosch, PhD, Rutgers University |
| 9:00 am |
Presentation of Historical
1963 Film: Insect Tissue Culture
T. D. C. Grace, PhD, CSIRO Entomologist (ret.), Canberra,
Australia |
| 9:30 am |
Invertebrate Cell Culture
Applications in China
Zhihong Hu, PhD, Wuhan Institute of Virology |
| 10:00 am |
Invertebrate Cell Culture
Biology and Novel Cell Lines
Robert R. Granados, PhD, Boyce Thompson Institute |
| 10:35 am |
Apoptosis Regulation In
Cultured Insect Cells
Rollie Clem, PhD, Kansas State University |
| 11:10 am |
Role of the Major Envelope
Protein (GP64) of Baculoviruses in Viral Entry and Exit
from Cultured Cells
Gary Blissard, PhD., Boyce Thompson Institute
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11:45 am
Break for Lunch
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12:00 pm –
1:30 pm
Luncheon Honoring Dr. T. D. C. Grace,
2004 Lifetime Achievement Award Recipient
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| 1:30 pm |
Transgenic Insect Lines
That Support Production of Humanized Glycoproteins by
Baculovius Expression Vectors
Donald Jarvis, PhD, University of Wyoming |
| 2:05 pm |
Stable Transformation
of Insect Cells with Densovirus Vectors and Expression
of Foreign Proteins
Max Bergoin, PhD, University of Montpellier II |
| 2:40 pm |
Scale-up and Optimizing
the In Vitro Growth of Insect Cells for Production of
Recombinant Proteins and Viral Pesticides
Spiros N. Agathos, PhD, University of Louvain |
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3:15 pm
Coffee Break
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| 3:35 pm |
Molecular Biology and
Genomics of Shrimp Viruses and Their In Vitro Culture
Just Vlak, PhD, Wageningen University |
| 4:10 pm |
Invertebrate Cell Cultures
for Commercial Pharmaceutical Drug Discovery
Steven H. Harwood, PhD |
| 4:45 pm |
Baculovirus Technology
for Mammalian Cell Gene Delivery
Patrick Condreay, PhD, GlaxoSmithKline Discovery
Research |
| 5:20 pm |
Summary and Comments
Dwight Lynn, PhD, USDA/ARS/BARC |
| 5:30 pm |
End of Conference
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Sunday,
May 23
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8:00 am – 10:00 am
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| Functional
Genomics of Aquatic Toxicology |
| Convener:
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J. Denry Sato, PhD, Mount Desert Island
Biological Laboratory |
| Fish represent a diverse
group of vertebrates living in a wide variety of environments
that vary with respect to oxygen tension, salinity,
temperature, pressure, light intensity and composition
of natural and manmade chemical toxicants. In recent
years, comparative studies of fish and mammalian genomes
have shown that these taxonomic groups, which diverged
over 400 million years ago, share much in the way of
conservation of genome organization and gene function.
Thus, it is likely that functional genomic studies of
evolutionary and physiological adaptations that allow
fish to live in diverse environments will also provide
insight into normal and pathological human physiology.
The talks in this session will present new tools and
organisms for studying the impact of aquatic environmental
toxicants on physiological processes. |
| Speakers:
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Comparative Approaches to Understanding Mechanisms
of Toxicity: The Comparative Toxicogenomics Database
(CTD)
Carolyn Mattingly, PhD, Mount Desert Island
Biological Laboratory
The Effects of Arsenic on the Function of CFTR Cl
Channels in Killifish, a Euryhaline Teleost
Bruce A. Stanton, PhD, Dartmouth Medical
School
“Fish & Chips” Using DNA Arrays to Study
Environmental Stress in Non-model Organisms
Andrew Gracey, PhD, Stanford University
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8:00 am – 10:00 am
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| Advances
in Automation of Cell Culture and Cell-based Assays |
| Conveners:
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Dennis A. Laska, Eli Lilly and Company,
and Linda B Jacobsen, PhD, Roche Diagnostics Corporation |
| The growth in application
of cell-based assays by academic and industrial life
science laboratories has led to automation advances
in both cell culture maintenance and assay conduct.
Automation of repetitive activities required to routinely
perform cell-based assays has help reduce the labor
intensity and open the bottleneck associated with these
assays. This session will introduce the participants
to several novel advances in automation technology,
as well as provide examples where implementation of
partial or total assay automation has led to increased
throughput, tighter data, and reduced costs. Additionally,
most automation equipment is designed in modular form
and can be tailored to fit specific current needs and
expanded in the future as need increases, thus these
opportunities are not limited to large core-laboratory
application or to industrial settings. |
| Speakers:
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Cost Benefit of Small Automation Systems in Cell-based
Laboratories
Joseph Bertoncini, Brandel Corporation
A Microcapillary Cytometry System for Cell-based Assays
Keith Olsen, PhD, Guava Technologies
Automation of Cellular Analysis and Gene Expression
Laura Pajak, PhD, Beckman Coulter, Inc.
Applications of Automated Cell Culture Technologies
to Enhance Assay Throughput
Linda B. Jacobsen, PhD, Roche Diagnostics
Corporation
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| 10:30 am – 12:30 pm
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World Congress Keynote Symposium
Chemically Programmable
Immunity: The Challenge and Approach of Today's Disease/Pathogen
Drug Treatments.
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| Speaker:
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Kary B. Mullis, PhD, Founder, Altermune,
LLC, Newport Beach, California
and 1993 Nobel Laureate in Chemistry For The Invention
of Polymerase Chair Reaction (PCR)
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3:00 pm – 5:00 pm
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| RNA
Interference: A New Tool in Gene Discovery and Gene
Therapy |
| Conveners: |
Harold N. Trick, PhD, Kansas State University,
Guy Smagghe, PhD, Ghent University, and Michael J. Fay,
PhD, Midwestern University |
| RNA interference or RNAi
is an innate cellular process activated when a double
–stranded RNA (dsRNA) molecule enters the cell,
causing the degradation of not only the invading dsRNA
molecule, but also single-stranded RNAs of identical
sequences, including endogenous mRNSs. This phenomenon
is apparently widespread in eukaryotes, ranging from
trypansome to human, from Neurospora to rice and has
sparked great interest from both fundamental and applied
perspectives. Currently, RNAi is being evaluated for
both functional genomic analyses and for its potential
in highly specific gene-silencing therapeutics. |
| Speakers: |
Applied Aspects of RNAi in C. Elegans
Wim Van Criekinge, Ghent University
RNAi-based Therapeutics
Barry Polisky, PhD, Sirna Therapeutics
Molecular Basis of Plant Defense Against Viruses
Vicki Bowman Vance, PhD, University
of South Carolina
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Monday,
May 24
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8:00 am – 10:00 am
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| RNA
Interference Workshop |
| Conveners: |
Janis Demetrulias, Technikos Research
Associates, and Michael J. Fay, PhD, Midwestern University
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| RNA interference (RNAi) describes
the phenomenon in which double stranded RNA (dsRNA)
promotes the postranscriptional destruction of homologous
mRNA. The resulting silencing or knockdown in the expression
of an individual gene represents a unique in vitro model
for investigating gene function. However, RNAi is more
than a helpful tool for gene function analysis. Small
RNAs are involved in chromatin regulation, gene expression
regulation during development, and are of importance
in the intrinsic cellular defense mechanism against
invading viruses. Science magazine considered RNAi as
one of the 2002 breakthroughs of the year. This workshop
will introduce many of the issues encountered on the
road to utilizing this new technology and will cover
such issues as target sequence selection, validating
knockdown, characteristics of different reagents, and
various applications of RNAi technology. |
| Speaker:
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John Rossi, PhD, Beck Research Institute
of the City of Hope
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10:30 am – 12:30 pm
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| Stem
Cells: Fundamental Aspects and Therapeutic Approaches |
| Convener: |
Gordana V. Vunjak-Novakovic, PhD, Massachusetts
Institute of Technology |
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Tissue engineering can potentially address tissue
and organ failure by implantation of biological substitutes
of native tissues. Engineered tissues can also serve
as physiologically relevant 3-dimensional models for
controlled studies of tissue development, remodeling,
and cell responses to genetic end environmental stimuli.
The clinical and scientific relevance of tissue engineering
critically depends on our ability to direct cells
to form specialized tissues that closely mimic native
physiology and can provide functional grafts for implantation.
This session will address some of the critical scientific
and clinical aspects of tissue engineering. The focus
is on (a) tissue engineering of functional arteries,
(b) establishment of human muscle stem cells and their
use for neural tissue engineering, and (c) state of
the art of orthopaedic tissue engineering.
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| Speakers:
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Biomimetic Approaches to Vascular Tissue Engineering
Laura Niklason, MD, PhD, Duke University
Immortalized Human Muscle Stem Cells and Their Potential
for Neural Tissue Engineering
Naohiro Hashimoto, Mitsubishi Kagaku Institute
of Life Sciences
Orthopaedic Tissue Engineering Basic Research and
Clinical Application
Ross Tubo, PhD, Genzyme Corporation
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| 1:30 pm – 3:00 pm |
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Cell Culture Systems for
Production of Pharmaceuticals
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| Conveners: |
Paul J. Price, PhD, GIBCO Invitrogen,
and David W. Jayme, PhD, GIBCO Invitrogen |
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The capability of cultured animal cells to support
high titer virus production and to synthesize and
secrete proteins with functional post-translational
modifications has facilitated the biopharmaceutical
production industry. The quantity and quality of product
yield is influenced by the exogenous environment,
requiring unique optimization of culture and expression
conditions for each cell type and target product.
Optimization may include the bioreactor type, nutrient
feeding strategy, environmental controls, and methods
for product harvest and waste removal. This mini-symposium
will invite thought leaders from three major Biotech
companies to present their experiences in maximizing
bioreactor yield for the manufacture of recombinant
proteins, monoclonal antibodies, and vaccines.
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| Speakers:
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Improving NS0 Fed-batch Culture Productivity
John Burky, PhD, Protein Design Labs, Inc.
Large Scale Perfusion Process Development: Platform
Technologies and Automation
Chun Zhang, PhD, Bayer Biological Products
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Tuesday,
May 25
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2:00 pm – 4:00 pm
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| Proteomics
Symposium: Biomarkers |
| Convener:
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Colette J. Rudd, PhD, Thermo Electron |
| What is a biomarker? A biomarker
is any molecule or group of molecules that differentiates
a particular state of a biological system, diseased
or healthy. Cancer, heart disease, inflammation and
infectious disease are all examples of situations in
which specific biomarkers might appear and accumulate
in cells and tissues. The majority of current research
typically focuses on biomarkers that are indicative
of cancer and are potentially useful for diagnosis,
prognosis, and/or therapy. Often biomarkers turn out
to be one or more proteins, such as prostate specific
antigen, that signify the presence of disease. Some
of these biomarkers, like the growth factor receptors
in breast cancer cells, also may become targets for
therapy using cytotoxic antibodies. Proteomic studies
attempt to identify and quantify the many different
proteins that comprise particular biological subsystems,
such as tissues, cells, and sub-cellular complexes.
Extracellular and secreted components are of particular
value for biomarkers because of their availability for
detection. New reagents, methods and instrumentation
have improved the ability of scientists to detect many
proteins in small biological samples, with or without
prior knowledge of the identity of the components. This
symposium will focus on the discoveries of biomarkers
for human disease that are evolving from advances in
proteomics, with emphasis on cell culture models as
a source of material for biomarker detection. |
| Speakers: |
Disease-specific Cell Surface Targets for Cancer
Diagnosis and Research
Jennie P. Mather, PhD, Raven Biotechnologies,
Inc.
Application of Proteomics in Technologies in Plant
Pathology
Bret Cooper, PhD, USDA-ARS
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4:00 pm – 5:00 pm
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| Functional
Proteomics Workshop: A Novel System for High-throughput
Protein Fractionation and Identification |
| Convener:
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Cynthia L. Goodman, USDA, ARS, BCIRL |
| This workshop will consider
a novel approach of how to fractionate and identify
proteins from complex solutions, such as cell lysates,
for both high throughput screening as well as standard
laboratory research programs. The specific method to
be discussed is based on a 2-D approach that utilizes
chromagraphy in place of standard electrophoretic gels.
Some advantages of this system include: high loading
capacity without band distortion; improved detection
of low-abundance species, membrane or hydrophobic proteins,
and low molecular weight proteins; enhanced reproducibility;
a contamination-free liquid flow path; simple automation;
and an ultra-high resolution analysis of complex protein
mixtures. Additionally, this technique can result in
the generation of detailed protein maps for later comparisons,
as well as the collection of liquid fractions that can
be either readily stored or further analyzed by mass
spectrometry. This session will involve a cohesive presentation
followed by a discussion session during which time questions
and comments will be encouraged. |
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Wednesday,
May 26
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| 10:30 am – 12:30 pm
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| Microscopy
for the 21st Century: New Ways to “See” Inside
the Cell |
| Convener: |
Alda Vidrich, PhD, University of Virginia
Health Sciences Center |
| Advances in our understanding
of cellular biology and cellular architecture have been
achieved, in large part, because of innovative engineering
in microscopic technology. Today, more than ever, the
microscope coupled with laser technology has become
a powerful tool in the study of molecular processes
and pathways at the single cell level. Proteomic and
genomic techniques are dependent on obtaining homogeneous
cell populations. Laser Capture Microdissection enables
the directed isolation of pure populations of cells
from heterogeneous samples. This technology allows cells,
cell aggregates, or discrete morphological structures
to be selected and capture from tissue sections. These
captured cells are suitable for nucleic acid studies
such as SNP analysis, endpoint and real-time RT-PCR
and mRNA expression profiling. Differential protein-expression
profiling by SDS-PAGE and 2D-PAGE as well as protein
identification by mass spectrometric sequencing, peptide
mass fingerprinting, in-gel zymography and Western blotting
also can be carried out with laser microdissected cells.
This SID and workshop will illustrated the range of
downstream applications in pathology, cancer research,
life sciences, medical diagnostics and biotechnology
for Laser Microdissection Technology as well as offer
a practical view of new advances in instrumentation
and sample handling. |
| Speakers:
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Laser Capture Microdissection and Analysis of Gene
Expression of Airway Mucous and Serous Gland Cells
Walter Finkbeiner, MD, PhD, San Francisco
General Hospital
Anne Mueller, PhD, Stanford University
Lewis Feldman, PhD, University of California
- Berkeley
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| 12:30 pm – 2:30 pm |
| Microscopy
Workshop |
| Convener:
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Alda Vidrich, PhD, University of Virginia
Health Science Center |
| Speakers: |
AS LMD New Advances in Laser Microdissection Technology
Andy Lee, PhD, Applications Specialist,
Leica Microsystems
Sample Preparation and Molecular Analysis for Laser
Microdissection Samples
Janice Zhou, PhD
Hands-on Demo will follow talks
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