2003 Congress on In Vitro Biology, Wednesday, June 4

Wednesday, June 4

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WEDNESDAY, JUNE 4

7:00 am – 5:00 pm

Registration

...........

Grand Ballroom Foyer

BIOMASS CONVERSION FOR FUELS

Conveners:

Michael E. Horn, Prodigene

Elizabeth E. Hood, Plant Biotechnologist

8:00 am – 10:00 am

Plant Symposium...............Washington/Clark

Fossilized hydrocarbon-based energy sources, such as coal, petroleum and natural gas, provide a limited,

non-renewable resource pool. Because of the worlds increasing population and increasing dependence on

energy sources for electricity and heating, transportation fuels, and manufacturing processes, energy

consumption is rising at an accelerating rate. Renewable resources, such as those derived from plants,

make economic and environmental sense, and we should investigate and implement models for moving to

these new energy sources. The goal is to derive 10% of our liquid fuels from renewable plant biomass by

2020, a 10-fold increase over todays production levels. This session will present current research in several

areas affecting our ability to produce fuels from lignocellulosic materials and the impact those issues have

on the economics of the process.

8:00

Introduction (M. Horn and E. Hood)

8:15

P-19

Transgenic Plant-produced Cellulases for Biomass Conversion

Elizabeth E. Hood, Plant Biotechnologist

8:45

P-20

The Impact of Feedstock Composition on Biomass Conversion Process Economics

Steven R. Thomas, National Renewable Energy Laboratory

9:15

P-21

Economics and Opportunities for Improvements for Biological Conversion of Cellulosic

Biomass to Ethanol

Charles E. Wyman, Dartmouth College

BIOTECHNOLOGY

Moderator:

Anton S. Callaway, North Carolina State University

Ryan Matthew Steeves, Kansas State University

8:00 am – 10:00 am Plant Contributed Paper Session...........

Multnomah

8:00

P-1030

Interactions Between Post-transcriptional and Transcriptional Silencing Pathways in

Arabidopsis thaliana

Anton S Callaway, North Carolina State University, William F. Thompson, George C.

Allen, Lindsay D. Jones, and Dolores, A. Sowinski

8:15

P-1031

A New Glyphosate Tolerance Strategy in Transgenic Crops

Michael W. Lassner, Verdia Inc., Daniel L. Siehl, Rebecca Gorton, Sean Bertain,

Hyeon-Je Cho, Donglong Liu, James Wong, Nick Duck, and Linda A. Castle

8:30

P-1032

Profiling of Differentially Expressed Genes in Yam (Dioscorea rotundata) During Post-

harvest Storage

Sali Kone-Coulibaly, Tuskegee University, M. Egnin, and G. He

8:45

P-1033

Chromatin Structure of T-DNA Integration Sites in Arabidopsis

Kirk E. Francis, North Carolina State University, and S. L. Spiker

9:00

P-1034

SSR Markers as a Suitable Tool for Checking Recombination Events During Somatic

Embryogenesis from Floral Explants in Grapes

Lucia Martinelli, Instituo Agrario Provinciale, M. S. Grando, J. Zambanini, V. Poletti, E.

Maffettone, and R. Marconi

9:15

P-1035

Transgenic “Sweet Rice” Expressing a Thermostable Amylopullulanase in Seeds Leads

to Starch Autohydrolysis and Production of Nutritionally-improved High-protein Flour

Su-May Yu, Academia Sinica, C.-M. Chiang, F.-S. Yeh, and J.-F. Shaw

10:00 am – 10:30 am

Coffee Break........

Mount St. Helen’s Foyer

TRANSGENES BLOWING IN THE WIND?

Convener:

Wayne Parrott, University of Georgia

10:30 am – 12:30 pm

Plant Symposium...............Washington/Clark

Pollen has been blowing in the wind ever since it first appeared on earth, facilitating gene flow among

plants. With the advent of transgenic crops, gene flow is being viewed increasingly not as a natural

phenomenon, but as something that must be avoided. Hence, gene flow and its control is playing a larger

role in the regulatory approval of transgenic crops. This session will present a talk on measuring gene flow

and determining its impact, another on the legal aspects of gene flow, and specific study of gene flow from

transgenic grasses.

10:30

Introduction (W. Parrot)

10:45

P-22

Transgenic Turfgrasses and Issues with Gene Flow

Eric K. Nelson, The Scotts Company

11:15

P-23

Gene Flow from Transgenic Crops to Wild and Weedy Relatives: When is It a Problem?

Allison A. Snow, Ohio State University

11:45

P-24

Liability Issues Related to Transgene Flow in Agriculture

Drew L. Kershen, University of Oklahoma College of Law

GENE SILENCING: USE FOR HIGH THROUGHPUT GENE VALIDATION AND/OR

FUNCTIONAL GENOMICS

Conveners:

Peggy J. Ozias-Akins, University of Georgia

Dwight T. Tomes, Pioneer Hi-Bred International, Inc.

Theodore M. Klein, Pioneer/DuPont Crop Genetics

2:30 pm – 4:30 pm

Plant Symposium...............Washington/Clark

Science magazine’s “Breakthrough of the Year: New Roles for RNAs” (2002. Science 298:2296) highlights

a rapidly emerging realization of the important and varied roles for small RNAs. Independent research in

plants and animals has converged to reveal related mechanisms for phenomena variously known as RNA

interference, quelling, co-suppression, or post-transcriptional gene silencing. The outcome, RNA-mediated

gene silencing, is similar for all of these phenomena, and involves double-stranded RNA that is recognized

by the host cell machinery as aberrant and is subsequently cleaved into smaller molecules. These smaller

molecules continue to propagate degradation in a sequence-dependent manner. RNA silencing in the form

of co-suppression was first recognized in plants, and the silencing of endogenous genes by homologous

transgenes remains a useful tool for exploring gene function. More recently, RNA silencing also has been

determined to play a role in plant development and defense. The use of RNA silencing as a tool for

functional genomics and its participation in natural biological processes will be addressed in this

symposium.

2:30

Introduction (P. Ozias-Akins, D. Tomes, and T. Klein)

2:45

P-25

Inhibition of Plant Gene Expression Technologies for Fun and Profit

Gregory P. Pogue, Large Scale Biology Corporation

3:15

P-26

Use of Transiently Expressed RNAi to Dissect Signaling Pathways in Plants

Tuan-Hua David Ho, Washington University

3:45

P-27

Plant MicroRNA’s and Their Targets

James Carrington, Oregon State University

NEW AND DEVELOPING TECHNOLOGIES FOR MICRO-PROPAGATION

Convener:

Bruce A. Stermer, PhD

2:30 pm – 4:30 pm

Plant Symposium..........................

Multnomah

Plant micropropagation by conventional technology is labor intensive and requires considerable inputs of

space and materials. However, new technologies are being developed that offer significant improvements

over conventional micropropagation. These emerging liquid culture systems can reduce the costs of labor

and materials, lessen the time required for production and also result in increased product quality. Today’s

session will present talks on the application of liquid culture systems for increased efficiency and quality in

plant product production.

2:30

Introduction (B. Stermer)

2:40

P-28

Somatic Embryo Development in Liquid Medium for Large-scale Propagation of Conifer

Trees

Pramod K. Gupta, Weyerhaeuser Company

3:10

P-29

Liquid Systems for Micropropagation, Storage, Shipping, and Acclimatization

Jeffrey W. Adelberg, Clemson University

3:40

P-1039

Gibberellin Synthesis Inhibitors Improve Conifer Embryogenic Tissue Initiation

Gerald S. Pullman, Institute of Paper Science and Technology

4:10

P-30

Alternative Media for the Micropropagation of Plants

Kenneth C. Torres, Phytotechnology Laboratories, L. Williams, N. Philman, and M.

Kane