2003 Congress on In Vitro Biology, Tuesday, June 3

Tuesday, June 3

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Posters

Daily Program
Program Summary
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Daily Program 		Poster Summary

 

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TUESDAY, JUNE 3
7:00 am – 5:30 pm
Registration
............Grand Ballroom Foyer
BIOREACTORS AND BIOPHARMING
Conveners:
Marceline Egnin, Tuskeegee University 
Mary Ann Lila, University of Illinois
8:00 am – 10:00 am 
Plant Symposium...............Washington/Clark
High value plant-derived pharmaceutical proteins promise to reduce human and animal suffering, and can
be manufactured using a delivery and production system that is safe, cost-efficient, and convenient.  Plants
can be engineered to produce drugs for disease prevention or therapy, nutraceutical or health-protective
compounds, or agents that may combat terrorism’s threats.  This session will overview the commercial
outlook for molecular farming approaches, the use of plants as bioreactors for production of uniform,
scaled-up quantities of active compounds, and the prospects for capitalizing on plants to produce agents
that can intervene in the event of biological or chemical warfare.  
8:00 
Introduction (M. Egnin and M. Lila)
8:15
P-13
Molecular Farming: Current Products and Future Prospects
Michael E. Horn, ProdiGene
8:45
P-14
Improving Nutraceuticals Through Tissue Culture
Alison M. R. Ferrie, Plant Biotechnology Institute
9:15
P-1036
Chemical Warfare Countermeasures: Expression of Human Acetylcholinesterase in
Plants
Samuel Patrick Fletcher, Arizona State University
9:35
P-1037
Plant-based Production of a Subunit Mucosal Vaccine for Pneumonic Plague
Bonnie Jean Woffenden, Virginia Tech
COUNTERING CHEMICAL AND BIOLOGICAL THREATS: CURRENT RESEARCH
Convener: 
Elizabeth J. Roemer, State University of New York – Stony Brook
8:00 am – 10:00 am 
Animal Symposium
........................
Multnomah
Continuing concern about the threat of chemical and biological agents both on the battlefield and as tools of
terror has lead to increased research in these arenas. Multiple agents ranging from organisms such as
anthrax and small pox; potent toxins including botulinum toxin and aflotoxin, and chemicals such as ricin,
sulfur mustard, and phosgene are all considered to pose potential dangers. Research is underway in a
variety of venues, both civilian and military, to develop better understanding of the mechanism of action of
these and others. Today’s session will present three talks on current, ongoing projects in the area of
chemical and biological threats. 
8:00
Introduction (E. Roemer)
8:15
VT-4
Ex Vivo Utilization of Dendritic Cells to Identify Vaccine Targets for Control of Infectious
Biothreat Agents
Kamal U. Saikh, US Army Medical Research Institute of Infectious Diseases
(USAMRIID)
8:45
VT-5
Evaluating Potential Inhibitors of Anthrax Lethal Factor Protein
Sanford R. Simon, State University of New York – Stony Brook
9:15
VT-6
Morphological Expression of Mustard Gas-induced Lesions In Vivo and In Vitro
John P. Petrali, US Army Medical Research Institute of Chemical Defense
(USAMRICD)
10:00 am – 10:30 am
Coffee Break.......................Grand Ballroom
10:00 am – 2:00 pm
Exhibits and Posters
................Grand Ballroom
ADVANCES IN CANCER MODELING AND TREATMENT
Conveners:
Richard Heller, University of South Florida
Paul J. Price, GIBCO Invitrogen
10:30 am – 12:30 pm 
Animal Symposium
........................
Multnomah
The first decade of this century may very well be the turning point in our diagnosis and treatment of cancer. 
Cancer models are allowing us to understand what changes are occurring when a normal cell becomes a
cancer cell and how different approaches may signal out the cancer cell for destruction.  It is the
understanding of unique or up-regulated pathways in the cancer cell that is allowing for targeted therapy. 
The knowledge of up-regulation of specific genes yielding up-regulated proteins and peptides is allowing for
the priming of the cytotoxic “T” to seek and destroy the tumor cells.  Cancer immunotherapy is predicted to
become a significant adjunct to other areas of cancer treatment.  The three speakers will cover these new
and exciting approaches to cancer treatment.
10:30
Introduction (R. Heller and P. Price)
10:45
VT-7
STI571: A Tyrosine Kinase Inhibitor for the Treatment of CML - Validating the Promise
of Molecularly Targeted Therapy
Michael W. N. Deininger, Oregon Health and Science University
11:15
VT-8
Dendritic Cell Immunotherapy of Cancer 
Reiner Laus, Dendreon Corporation
11:45
VT-9
Identification of Casual Genetic Alterations in Human Breast Cancer Using New Model
Cell Lines and Xenografts
Stephen P. Ethier, University of Michigan Cancer Center
DISEASE AND PEST: PATHWAYS TO RESISTANCE
Convener: 
Heidi F. Kaeppler, University of Wisconsin
10:30 am – 12:30 pm 
Plant Symposium...............Washington/Clark
Pathogens and insects are the major causes of crop quality and yield losses worldwide.  Breeding efforts
aimed at enhancing resistance to diseases and pests have been successful in many cases.  Continued
research aimed at improving resistance is needed, however, because current levels of resistance are
inadequate, the resistance is of a nondurable form and/or sources of adequate resistance are unavailable
within the crop species germplasm.  Expression of simple antifungal proteins in transgenic plants has
resulted in mixed effects on resistance, and has led to investigation of more complex transgenic strategies
for enhancing resistance. Detailed characterization of plant defense genes and regulatory cascades should
result in improved design of genetic resistance strategies, both for breeding and transgenic approaches
toward enhancing resistance.  The speakers in this symposium will discuss findings from investigations of
plant defense products and pathways, and how results can be used for enhancing plant resistance to
pathogens and pests.
10:30
Introduction (H. Kaeppler)
10:45
P-15
The XA21 Receptor Kinase Mediated Defense Response in Rice
Pamela C. Ronald, University of California – Davis
11:15
P-16
Lipid Signaling in Plant Defense
Jyoti Shah, Kansas State University
11:45
P-17
The Interface Between Bacterial Pathogens and Plants: Virulence Functions and
Resistance Responses
David Mackey, The Ohio State University
PLANT TISSUE CULTURE AND MICROPROPAGATION
Moderator:  
Baochun Li, University of Kentucky
M. Manoharan, USDA/ARS
10:30 am – 12:45 pm
Plant Contributed Paper Session
.........................................
Clackamas
10:30
P-1022
Shoot Organogenesis in Nicotiana Species: Shoot Production per Responsive Leaf
Explant Increases Exponentially with Explant Organogenic Potential
Baochun Li, University of Kentucky, W. (Q. W.) Huwang, and T. Bass
10:45
P-1023
Micropropagation of Cordyceps sinensis (Berk) Sacc., a High Value Medicinal Fungus
Wildly Growing in Himalayan Region
Narendra Kumar, Government of India – Ministry of Defence, and P. S. Negi
11:00
P-1024
Micropropagation Saves the Endangered Musa Germplasm in the FSM
Hattie Andrew, Micronesia Plant Propagation Research Center, P. C. Josekutty, N. H.
Nena, R. A. George, T. N. Kilafwasru, and S. S. Cornelius
11:15
P-1025
Micropropagation and Field Performance Evaluation of Eight Micronesian Bananas
Puthiyaparambil Chacko Josekutty, Micronesia Plant Propagation Research Center
11:30
P-1026
In Vitro Initiation of Artocarpus heterophyllys Lam. (Jak Fruit) – Effect of the Explant
Type and the Season of Explant Collection
W. T. P. S. K. Senarath, Chonbuk National University, K. A. H. K. Kasturiarachchi, and
Kui Jae Lee
11:45
P-1027
Shoot Tip Culture: A Powerful Model System for In Vitro Flowering and Transformation
Studies in Maize (Zea mays L.)
Swati Bhargava, G. B. Pant University of Agriculture and Technology, Alok Shukla, and
R. C. Pant
12:00
P-1028
Micropropagation of Papuacalia versteegii, an Important Endemic Plant to Mount Jaya
Erly Marwani, Institute of Technology Bandung, and W. Sarosa
12:15
P-1029
Somatic Embryogenesis from Ovules of Kinnow (Citrus nobilis X C. deliciosa) for
Elimination of ICRSV
Gita Rani, Guru Nanak Dev University, B. Singh, S. Sharma, A. A. Zaidi, V. Hallan, A.
Nagpal, and G. S. Virk
12:30
P-1040
A Comparative Account of the Studies on Embryogenesis and Organogenesis in
Various Cultivars of Cotton (Gossypium spp.)
Tanveer Khan, G. B. Pant University of Agriculture and Technology, and R. C. Pant
CELL MODELS AND CELLULAR DIFFERENTIATION
Moderator:  
Kim C. O’Connor, Tulane University
1:30 pm – 3:00 pm
Joint Vertebrate/Toxicology Contributed Paper Session.........
Clackamas
1:30
VT-1000
Differentiation Kinetics of In Vitro 3T3-L1 Preadipocyte Cultures
Kim C. O’Connor, Tulane University, H. Song, K. D. Papadopoulos, and D. A. Jansen
1:45
VT-1001
Characterization of I-11.15, an Immortalized Murine Splenic Macrophage Cell Line
Aicha Delafoulhouse, California State University at Fullerton, C. Calumpong, and D. B.
Drath
2:00
VT-1002
Improving the Embryonic Stem Cell Test (EST) by Establishing Molecular Endpoints of
Tissue-specific Development
Roland Buesen, Federal Institute for Risk Assessment, A. Seiler, A. Visan, B. Slawik,
E. Genschow, and H. Spielmann
2:15
VT-1003
Growth and avb3 Expression In OPC1 Cells Grown on Thermanox® Plastic and
Collagen Substrates
Jessica Lee Moore, Washington State University, C. Davitt and H. Hosick
2:30
VT-1004
The Extracellular Matrix Laminin, Fibronectin, and Collagen IV  in Green Sea Turtle
Gonadal Cell Cultures
Anggraini Barlian, Institut Teknologi Bandung, S. Sudarwati, L. A. Sutasurya, and H.
Hayashi
1:30 pm – 2:00 pm 
All Poster Authors will be present
..................................Grand Ballroom
2:00 pm – 3:00 pm
Poster Breakdown and Removal
..................................Grand Ballroom
2:00 pm
City of Roses Silent Auction Final Bidding.............Grand Ballroom Foyer
DELIVERY OF GENES TO MAMMALIAN CELLS WITH BACULOVIRUSES
Convener:
Guido F. Caputo, Natural Resources Canada
3:00 pm – 5:00 pm 
Animal Symposium
........................
Multnomah
3:00
Introduction (G. Caputo)
3:15
I-7
Isolation and Characterization of Transgenic Insect Cell Lines with Humanized
Glycoprotein Processing Pathways 
Donald L. Jarvis, University of Wyoming
3:45
I-8
Recombinant Baculoviruses as Mammalian Cell Gene-delivery Vectors
Tom A. Kost, GlaxoSmithKline
4:15
I-9
Enhancing the Sensitivity of Rainbow Trout Cells in Culture to the Toxicity of Metals
Vivian Rashida Dayeh, University of Waterloo
PREDICTIVE TOXICOLOGY
Moderator: 
John W. Harbell, Institute for In Vitro Science, Inc.
3:00 pm – 5:00 pm 
Animal Roundtable Session
..................
Timberline
Traditionally, application of in vitro methods to drug discovery or toxicology often focused on an
understanding of the mechanisms underlying the changes in target tissues or organs occurring in vivo.
Increasingly though, in vitro methods are being applied prospectively to predict the action of a chemical on
the target tissue or whole organism. There are several forces driving the increased emphasis on in vitro cell
and tissue based models and accompanying test procedures.  One primary force has been the need to
support the high through put screens to address the chemical libraries developed from combinatorial
chemistry. The large number of potential active compounds and the small quantity of each chemical have
precluded most in vivo assessments. Concerns about extrapolation across species have also increased the
focus on human cell systems, especially in organ-specific and metabolism studies. In vivo, absorption,
distribution, metabolism and excretion (ADME) are measured to determine the effective dose and duration
of exposure to the parent compound and any active metabolites. To predict the potential action of the
chemical in vitro, the ADME factors must be addressed along with the action on the target tissue(s). This
symposium will focus on the in vitro approaches to assessing intestinal absorption, hepatocyte metabolism
(phase 1 bioactivation and phase 2 inactivation) as well as basal and organ-specific toxicity.
3:00
Introduction (J. Harbell)
3:15
W-4
The Caco-2 Assay: An In Vitro Models for Prediction of Intestinal Permeability
Dennis A. Laska, Eli Lilly and Company
3:45
W-5
Hepatocyte Systems for Predicting First-pass Metabolism and Bioavailability of
Chemicals In Vitro
Edward L. LeCluyse, University of North Carolina
4:15
W-6
Model Systems for Cytotoxicity Screening and Predicting Target Organ Effects
Charles A. Tyson, SRI International
TRANSGENIC CEREALS
Conveners:
Mark C. Jordan, Agriculture & Agri-Food Canada
Harold N. Trick, Kansas State University
3:00 pm – 5:00 pm 
Plant Symposium...............Washington/Clark
The present large-scale commercial production of transgenic maize is largely dependent on advances in
transformation technology leading to high throughput transformation systems. The procedures and
practices for selection of an appropriate transformation system in maize will ultimately be applied to other
cereals such as wheat. The next bottleneck in cereals is the ability to isolate genes for important traits due
to the large and complex genome. In spite of this, wheat is now coming into its own as a tool for functional
genomics. Several international programs in wheat genomics have generated a wealth of sequence
information and expression data. Transgenic wheat is poised to play a major role in the assignment of
function to these sequences and the potential to develop gene tagging strategies in wheat will be
discussed. After successful transformation and gene identification strategies are in place for wheat, there
are still barriers to commercial production. There is no commercial production of transgenic wheat in the
world today; however, there are products in advanced field trials that could be ready for release in 2004.
Given potential impacts on international export markets as well as other issues, there has been pressure
exerted on governmental agencies to prevent the release of transgenic wheat. Herbicide tolerant transgenic
wheat has been bred and tested in the Pacific Northwest and we will hear from a wheat breeder who has
worked with the product and can discuss the risks and benefits. 
3:00 
Introduction (M. Jordan and H. Trick)
3:15
P-17A
Gene Identification in Wheat
Camille M. Steber, USDA-ARS, Washington State University
3:45
P-1038
Statistical Analysis of Frequency and Quality of Transgenic Maize Production from
Three Transformation Methods: Agrobacterium, Gunpowder Gun, Electric Gun
David D. Songstad, Monsanto Company
4:15
P-18
Risk Assessment of RoundUp® Ready Wheat Production in the Pacific Northwest
Kimberlee K. Kidwell, Washington State University
5:00 pm – 6:00 pm
SIVB Business Meeting ..........................................................
Multnomah
(All Members are Urged to Attend)
7:00 pm – 8:00 pm
Reception
...............................................................Grand Ballroom Foyer
7:00 pm – 8:00 pm
Announcement of 
City of Roses Silent Auction Winners ...............Grand Ballroom Foyer
8:00 pm – 10:00 pm
Closing Banquet ...............................................................Grand Ballroom
Seating is Limited.  Admittance to Banquet by Advance Ticket Holders Only.