2003 Congress on In Vitro Biology, Sunday June 1
SUNDAY, JUNE 1
7:00 pm – 6:00 pm
Registration
............Grand Ballroom Foyer
CRYOPRESERVATION
Conveners: 
Barbara M. Reed, USDA-ARS 
Valerie C. Pence, Cincinnati Zoo and Botanical Garden
Lia H. Campbell, Organ Recovery Systems, Inc.
8:00 am – 10:00 am 
Joint Symposium
...............Washington/Clark
Techniques for storing living materials in liquid nitrogen have become important tools in the areas of agriculture,
medicine, and conservation, and in vitro systems are often particularly suited for long-term storage at freezing
temperatures. Research into cryoprotective and recovery mechanisms, while dealing with specific taxa, can
provide guidance for the application of cryopreservation to a variety of systems. This session will provide an
update on recent work on oxidative stress and desiccation tolerance in cryobiological systems as well as important
principles needed for successful cryopreservation.
8:00 
Introduction (B. Reed, V. Pence, and L. Campbell)
8:15
J-1
The Role of Small Amphiphilic Solutes in Desiccation Tolerance 
Ann E. Oliver, University of California – Davis
8:45
J-2
Cryopreservation Principles for Mammalian Tissues: The Basis of Success or Failure
Michael J. Taylor, Organ Recovery Systems, Inc.
9:15
J-3
A Free Radical View of Cryopreservation
David H. Bremner and Erica E. Benson, University of Abertay Dundee
10:00 am – 10:30 am
Coffee Break.......................Grand Ballroom
10:00 am – 3:00 pm
Exhibits and Posters
................Grand Ballroom
CREATING CONNECTIONS BETWEEN SCIENTISTS AND
EDUCATORS: BRINGING BIOTECH TO THE CLASSROOM
Convener:  
Carol M. Stiff, Kitchen Culture Kits, Inc.
10:30 am – 12:30 pm 
Education Symposium.......................
Timberline
Incredible advances are being made in the fields of plant, vertebrate, and invertebrate in vitro biology. It is our duty
as scientists to insure that instructional resources, conceptual background information, and hands-on laboratory
experiences can be incorporated into classrooms. This serves to both educate the public and foster development
of our future scientists and educators. The session will discuss the various ways this has been accomplished and
will provide web resources for continued networking.
10:30
Introduction  (C. Stiff)
10:45
E-1
Connecting with Teachers Through Hands-on Workshops, Online Courses and Listservs, and
User-friendly Kits
Carol M. Stiff, Kitchen Culture Kits, Inc.
11:10
E-2
LIGASE Loaners: Bringing Scientific Expertise to the High School Classroom 
Patricia E. Bossert, Northport/East Northport School District
11:35
E-3
Creating a Viable Biotechnology Program in High School 
Judi Heitz, San Diego High School
12:00
E-4
Bio-Link: Promoting Biotechnology Education in Community Colleges
Beth Pitonzo, Mount Hood Community College
THE FUTURE OF SERUM: A ROUNDTABLE DISCUSSION
Convener: 
William J. Smith, U.S. Army Medical Research Institute of Chemical Defense
10:30 am – 12:30 pm
Animal Symposium
........................
Multnomah
Serum has been a critical supplement to cell culture media for many years. Developments in serum collection and
preparation have refined the quality of the product, but questions persist as to the standardization and purity of
serum in critical cell culture studies. Many efforts have been made to produce serum-free or defined media. A
number of successes have resulted for a limited span of cell types. The panel, consisting of representatives of
three major cell culture media and reagent suppliers, will help us understand the problems and benefits associated
with the use of serum in culture systems. They will also give us insights into what the future holds in the
formulation of media and the selection of supplements.
Panelists: 
David Jayme, Invitrogen Corporation
Bill B. Barnett, HyClone Laboratories
Gary Shipley, Cascade Biologics, Inc.
HIGH THROUGHPUT METHODS
Conveners: 
Allan R. Wenck, Syngenta 
Ebrahim Firoozabady, Del Monte Fresh Produce
10:30 am – 12:30 pm 
Plant Symposium...............Washington/Clark
We have entered an era where high quality sequence is being made available to researchers throughout the world.
The rice, Arabidopsis, and soon other sequences will be known. As methods improve, more and more information
will flow through the scientific databases. How do we deal with this sequence information? How can science make
biological sense out of the genetic code and identify important areas of interest? How are we to identify and
validate genes with potential impact on such things as yield, stress tolerance and pest resistance? Computer
programs are in place to help sift out potentially important sequences from the billions of base pairs into
thousands or tens of thousands of genes. This subset of genes needs to be expressed in a high throughput
manner within biological systems generating thousands or tens of thousands of events for analysis. Both molecular
and other characterization data must be collected in a high throughput manner in order to validate the predicted
functions of these chosen sequences. This session will provide examples of how we can identify potential genes of
interest, transform them in a high throughput manner, and characterize them in an equally high throughput
manner.
10:30
Introduction (A. Wenck and E. Firoozabady)
10:45
P-1
High-throughput Methods for Determining Transgene Copy Number and Expression in Plants
Wen-Jin Yu, Syngenta Biotechnology, Inc.
11:15
P-2
A Maize “Whiskers” Transformation System
W. Paul Bullock, Garst Seed Company
11:45
P-3
High Throughput Plant Gene Function Analysis
Keith Davis, Paradigm Genetics, Inc.
IN VITRO TOOLS
Moderators:  
Bernadette Lourdes Plair, Cincinnati Zoo and Botanical Garden 
Maureen M. M. Fitch, USDA/ARS
10:30 am – 12:30 pm
Plant Contributed Paper Session
.........................................
Clackamas
10:30
P-1000
Development of an In Vitro Tuberization System for Sweet Potato Micro-storage Root
Formation
Latrice Crawford, Tuskegee University,  Marceline Egnin, and Anne Sama
10:45
P-1001
Cryopreservation of Shoot Tips of the Endangered Asimina tetramera by Encapsulation-
vitrification
Bernadette Lourdes Plair, Cincinnati Zoo and Botanical Garden, and V. C. Pence
11:00
P-1002
In Vitro Collecting and Establishment of Tissue Culture Lines of Three Endangered Florida
Pawpaws
Valerie C. Pence, Cincinnati Zoo and Botanical Garden, and S. M. Charls
11:15
P-1003
Control of Bacterial Contamination in Large Scale Papaya Micropropagation
Maureen M. M. Fitch, USDA/ARS, T. Leong, N. Saito, G. Yamamoto, A. Dela Cruz, A. Yeh, S.
White, S. Maeda, S. Ferreira, and P. Moore
11:30
P-1004
Factors Affecting In Vitro Establishment of Cocoyam (Xanthosoma sagittifolium L Schott)
Anne Eyango Sama, Tuskegee University, M. Egnin, and S. Zok
11:45
P-1005
Somaclonal Variation Detection and Chimerism in Somatic Embryo-derived Cocoa
Carlos Marcelino Rodriguez Lopez, Reading University, M. J. Wilkinson, and A. C. Wetten
12:00
P-1006
Encapsulation of Orchid-Phalaenopsis Shoot Tips for Storage and Exchange of Germplasm
W. T. P. S. K. Senarath, Chonbuk National University, Kui Jae Lee, and S. Rehman
12:15
P-1007
A Novel Disposable Film Culture Vessel for Photoautotrophic Micropropagation of Epidendrum
Orchid
Giang Thi Thanh Dam, Kagawa University, H. Watanabe, M. Ujike, Y. Kume, and M. Tanaka
1:30 pm – 2:30 pm
Non-interactive Even Poster Authors will be present
.......Grand Ballroom
Interactive Poster Authors listed below will be present during their sessions
IN VITRO TOOLS AND TECHNIQUES
Moderator:
John W. Harbell, Institute for In Vitro Sciences, Inc.
1:30 pm – 2:30 pm
Joint Interactive Vertebrate/Toxicology Poster Session...Grand Ballroom
VT-2000
An Animal Origin Free Trypsin Alternative to Harvest Cells
Lori L. Nestler, Invitrogen Corporation, E. K. Evege, J. A. McLaughlin, D. G. Munroe, T. C.
Tan, K. E. Wagner, and B. Stiles
VT-2001
A Filter for Trapping Metastatic Breast Cancer Cells
Jessica L. Moore, Washington State University, P. Elias, C. Davitt, A. Bandyopadhyay, S.
Bose, S. Kalita, and H. Hosick
VT-2002
Induction of a Zone of Cell Death in Multi-well Plates by Refeeding
John W. Harbell, Institute for In Vitro Sciences, Inc., H. Raabe, G. Moyer, G. Mun, and M.
Clear
VT-2003
Comparative Cytotoxcity of Three In Vitro Cell Viability Assays
Ann M. Wright, CiBA Vision/Novartis Company, and Mary Mowery-McKee
VT-2004
Mycoplasma Testing Experience of an Academic Support Facility
Margaret L. Smith, Riggs Consulting, Lovella Cacho, and Phan Tu
VT-2005
Use of a Self-assembling Pore for the Indtroduction of Impermeant Molecules through
Mammalian Cell Membranes
Lia H. Campbell, Organ Recovery Systems, K. Sarver, K. Ratcliff, M. J. Taylor, J. Walsh, and
K. G. M. Brockbank
VT-2006
Enhanced Effectiveness of Non-viral Gene Transfer Using Electroporation
Richard Heller, University of South Florida, Richard Gilbert, Kathleen Merkler, and Loree Heller
VT-2007
Confocal Imaging of Epidermal Growth Factor Peptide Binding Along the Stem Cell
Compartment of Mammalian Colonic Crypt
Bertrand A. Kaeffer, Institut National Recherche, Lissia Pardini, and Alain Trubuil
VT-2015
Protein Kinase C Disrupts the Formation of VA Gene Transcription Initiation Complex
Calvin B. James, Ohio University
INSECT MIDGUT STEM CELL LINES AND INSECT CELL LINES FOR BIOCONTROL
Moderator:
Guy Smagghe, Ghent University
1:30 pm – 2:30 pm
Interactive Invertebrate Poster Session.............................Grand Ballroom
I-2001
Effect of Bt Proteins on the Viability of Selected Insect Cell Lines
Cynthia L. Goodman, USDA/ARS/BCIRL, H. Nabli, J. Baum, T. Malvar, B. Isaac, Y.-J. Lee, A.
H. McIntosh, and S. J. Phipps
I-2002
Insulin-like Peptides Stimulate Midgut Stem Cell Proliferation of Lepidopteran Larvae In Vitro
Shintaro Goto, Kobe University, Marcia J. Loeb, and Makio Takeda
I-2003
Effects of Insect Hormone Actions, 20E and JH, on Midgut Stem Cells of Lepidoptera
Guy Smagghe, Ghent University, W. Vanhassel, C. Moeremans, K. Elsen, and M. Loeb
I-2004
Who Controls Midgut Stem Cell Differentiation – the Stem Cell or the Environment?
Raziel S. Hakim, Howard University, M. Loeb, and J. Young
STRESS BIOLOGY
Moderator:
Lynn S. Dahleen, USDA/ARS
1:30 pm – 2:30 pm
Interactive Plant Poster Session.......Grand Ballroom
P-2000
Transformation of Barley with Two Antifungal Genes
Lynn S. Dahleen, USDA/ARS, and M. Manoharan
P-2001
Regeneration and Genetic Transformation of Durum Wheat
M. Manoharan, USDA/ARS, L. S. Dahleen, and P. B. Jauhar
P-2002
Effects of UV-B on the Development of In Vitro Propagated African Violet Chimera (S. ionantha)
Wai-Yei Leung, Champlain Regional College Saint Lambert, P. Castillo-Ruiz, E. Belanger, and
S. Taylor
P-2003
Overexpression Antioxidant Gene in Tomato Increases Tolerance to Heat and Chilling Stress
Yueju Wang, Oregon State University, Michael Wisniewski, Lailiang Cheng, Richard Meilan,
Minggang Cui, and Leslie Fuchigami
P-2004
Expression of the Arabidopsis CBF1 Gene in Poplar Confers Elevated Freezing Tolerance
Yongjian Chang, Oregon State University, Jeffery S. Skinner, and Tony H.H. Chen
PLENARY SESSION
Convener:  
William J. Smith, US Army Medical Research Institute of Chemical Defense
3:00 pm – 4:30 pm
Plenary Session.......
Multnomah/Clackamas
ARTistic Use of Nonhuman Primates:
IVF to Cloning and Beyond
3:00
Introduction: 
William J. Smith, 2003 Congress Program Chair
Opening Remarks: Sandra L. Schneider, Research and Clinical Systems and President, Society for In
Vitro Biology
PS-1 
Plenary Speaker: 
Richard Stouffer, Head of the Reproductive Sciences Division, Oregon Regional
Primate Research Center
5:00 pm – 9:00 pm
PLENARY RECEPTION AND DINNER ...................Pittock Mansion and
The World Forestry Center