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2003 Congress on In Vitro Biology, Monday June 2
Monday, June 2
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Monday, June 2
7:00 am – 6:00 pm
Registration
...........
Grand Ballroom Foyer
GROWTH FACTORS IN GROWTH,
REGENERATION, AND DIFFERENTIATION OF
INVERTEBRATE CELLS
Conveners:
Marcia J. Loeb, USDA
Raziel S. Hakim, Howard University
8:00 am – 12:30 pm
Animal Symposium
.........................
Timberline
Growth factors play a large role in regulating the growth, development, and differentiation of mammalian
tissues. It is only recently that the roles of peptidic growth factors in the growth, development, and
differentiation of invertebrates have been revealed. There are a very few known factors that are specific to
development in insects and molluscs; most of the invertebrate organisms that have been studied in this
respect are controlled by the same factors that regulate vertebrates. Their mechanisms of action for those
invertebrates, such as Caenorhabditis elegans, that have been extensively characterized, are similar as
well. Therefore, invertebrate tissues may serve as models for vertebrate organisms. In addition,
invertebrate pest control agents whose mechanisms involve manipulation of growth factors will have to be
studied in depth in order to avoid doing harm to the general environment where all animals have to live in
harmony with each other.
8:00
Introduction (R. Hakim)
8:15
I-1
Co-culture of Trypanosoma musculi with Spleen-derived Adherent Fibroblasts and
Macrophages Ensures Survival In Vitro
Winston A. Anderson, Howard University
8:45
I-2
Lymnaea EGF, Roles in Neurite Outgrowth In Vitro and In Vivo
Andrew G. M. Bulloch, University of Calgary
9:15
I-3
Growth-blocking Peptide Family and Its Multiple Effects on Insect and Vertebrate Cells
Yoichi Hayakawa, Hokkaido University
9:45
Discussion
10:00 – 10:30
Invertebrate Session Coffee Break.....Grand Ballroom
10:30
I-4
Regeneration of Walking Legs in the Fiddler Crab, Uca pugilator: The Role of Growth
Factors
Penny M. Hopkins, PhD, University of Oklahoma
11:00
I-5
Regulation of the Fate of Stem Cells from the Midgut of the Caterpillar, Heliothis
virescens
Marcia J. Loeb, USDA
11:30
I-6
Modulation of Growth Factor Signaling During C. elegans Vulva Development
Nadeem Moghal, California Institute of Technology
BIOPHARMACEUTICAL MANUFACTURING:
ADDRESSING THE NEEDS FOR STAFFING,
FACILITIES, AND PROCESSES DEVELOPMENT
Moderators:
Dennis A. Laska, Eli Lilly and Company
William J. Smith, U.S. Army Medical Research Institute of
Chemical Defense
8:00 am – 10:00 am
Animal Roundtable Session
.................
Mutlnomah
Current bottlenecks and future needs for state of the art biopharmaceutical manufacturing facilities coupled
with shortages of highly trained technical and professional staff threaten to slow or even impede
development and commercialization of greatly needed bio-molecules, vaccines, and therapeutics. This
roundtable session will address in depth the current situation, pose scenarios for remediation, and project
future trends in professional and technical training, process improvement and efficiency, as well as quality
initiatives.
8:00
Introduction (D. Laska)
8:15
W-1
Academic Institutions Response to the Staffing Needs of the Biopharmaceutical Industry
Kamal A. Rashid, Biotechnology and Genomics Research Center
8:45
W-2
Industrial Mammalian and Microbial Cell Culture System for Biopharmaceutical
Manufacture of Therapeutics
Sarad Parekh, Dow AgroSciences, LLC
9:15
W-3
Ways to Approach Process Development and Improve Production Efficiency in
Mammalian Cell Culture
Julia Cino, New Brunswick Scientific Co., Inc.
9:45
Questions and Discussion
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BIOTECHNOLOGY OF GRAPEVINE
IMPROVEMENT
Convener:
Cecilia Zapata, Yoder Brothers, Inc.
8:00 am – 10:00 am
Plant Symposium...............Washington/Clark
Techniques for genetic modification in grapevine (Vitis spp.) using somatic embryogenesis (SE) include
transformation and in vitro selection. All successful examples of genetic transformation in grapevine have
utilized embryogenic cells as targets for gene insertion and somatic embryos for recovery of transformed
plants. Transgenics have been used to solve problems with disease and stress resistance as well as
manipulation of qualitative traits. In vitro selection has also been used as an alternate method to select for
disease resistance in grapevine. In the area of grape genomics, incredible advances have been made in
the recent past, where an estimate of 44,928 grape (Vitis vinifera) ESTs have been produced. These code
for approximately 18,500 grape genes, representing 2/3 of the grape genome. This symposium will present
a talk on the uses of somatic embryogenesis and transformation for grapevine improvement. The session
will include another presentation on in vitro selection to enhance disease resistance. It also includes a
presentation on the grape genome project focusing on gene discovery.
8:00
Introduction (C. Zapata)
8:15
P-4
Applications of Somatic Embryogenesis and Transformation in Grapevine Improvement
Dennis J. Gray, University of Florida
8:45
P-5
Gene Discovery in Grapes: The Grape Genome Project
Effie Ablett, Southern Cross University
9:15
P-6
In Vitro Selection to Enhance Disease Resistance in Grapevine
Jayasankar Subramanian, University of Guelph
Moderators:
Jane Vishnevetsky, The Volcani Center
Diaa F. Al-Abed, The University of Toledo
8:00 am – 10:00 am Plant Contributed Paper Session...........
Clackamas
8:00
P-1008
Transformation and Regeneration of Wetland Monocot Juncus accuminatus Using
Different Binary Vectors
Li Chen, Salem International University, R. Nandakumar, P. K. Lai, and S. M. D. Rogers
8:15
P-1009
An Efficient System for Biolistic Transformation and Plant Regeneration of Pearl Millet
Using Spikelets Shaved from Immature Inflorescences
Jason James Goldman, University of Georgia, W. W. Hanna, G. Fleming, and P.
Ozias-Akins
8:30
P-1010
Alleviation of Wheat Allergenicity Using the Thioredoxin System
Hyun-Kyung Kim, University of California-Berkeley, M.-J. Cho, H. R. Jung, Y.-B. Kim,
S. Morigasaki, J. H. Wong, P. G. Lemaux, and B. B. Buchanan
8:45
P-1011
Transgenic Spirodela: A Unique, Low-risk, Plant Biotechnology System
Avihai Perl, Volcani Center, Marvin Edelman, Ron Vunsh, Jihong Li, Uri Hanania,
Moshe Flaishman, and Jane Vishnevetsky
9:00
P-1012
Expression of a Synthetic Avidin Gene in Maize for Control of Corn Rootworm
(Diabrotica spp.) and Other Insect Pests
Serena B. McCoy, Kansas State University, Mitsuhiro Ueda, Karl J. Kramer,
Subbaratnam Muthukrishnan, and Harold N. Trick
9:15
P-1013
Shoot Meristem: An Ideal Explant for Zea mays (L.) Transformation
Diaa F. Al-Abed, The University of Toledo, R. V. Sairam, and S. L. Goldman
9:30
P-1014
Enhanced Fungal Tolerance in Transformed Banana (Musa spp. AAA cv. ‘Grand Nain’)
Plants Regenerated Through Somatic Embryogenesis
Jane Vishnevetsky, The Volcani Center, Y. Cohen, M. A. Flaishman, and A. Perl
10:00 am – 10:30 am
Coffee Break.......................Grand Ballroom
10:00 am – 3:00 pm
Exhibits and Posters
................Grand Ballroom
EPIGENETIC TOXICANTS, ALTERED
INTRA-CELLULAR SIGNALING AND MODULATED
GAP JUNCTIONAL INTER-CELLULAR COMMUNICATION
Convener:
Alda Vidrich, University of Virginia Health System
10:30 am – 12:30 pm
Animal Symposium
........................
Multnomah
Many chemicals capable of inducing multiple health risks do so without causing genetic mutations. The
mechanisms whereby these chemicals cause disease apart from mutagenesis include cytotoxicity (either
necrosis or apoptosis) and epigenetic toxicity. Via epigenetic toxic mechanisms chemicals can alter gene
expression at the level of transcription, translation or post-translational events in a stem cell, a precursor
cell or a terminally differentiated cell. This session will explore the role of intercellular signaling and
communication in the cellular response to epigenetic toxicants as well as the mode by which epigenetic
toxicants can alter the channels of intercellular communication.
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10:30
Introduction (A. Vidrich)
10:45
VT-1
Gap Junctions, Homeostasis, and Epigenetic Toxicology
Randall J. Ruch, Medical College of Ohio
11:15
VT-2
Integrated Signaling Effects of Epigenetic Toxicants
Brad Upham, Michigan State University
11:45
VT-3
Stem Cells, Cell-cell Communication and Epigenetic Toxicants: Risk Assessment
Implications
James E. Trosko, Michigan State University
FOREST BIOTECHNOLOGY: SHOULD,
COULD, AND WOOD
Conveners:
Todd J. Jones, Weyerhaeuser Technology Center
Maud A. Hinchee, ArborGen
10:30 am – 12:30 pm
Plant Symposium...............Washington/Clark
Researchers now have the tools to apply genetic engineering and biotechnology for the improvement of
commercially important forest tree species. We are currently on the threshold of major advancements in our
understanding of gene function in trees. The Poplar Genome Sequencing Project is the first full-scale
sequencing of a forest tree species and it will provide the fundamental basis for functional tree genomics.
This project, which is nearing completion, will add thousands of tree genes to the molecular toolbox and
provide new insights into what genes contribute to commercially valuable tree phenotypes. For some
biochemical pathways, we already know which genes encode many of the important enzymes. One such
pathway is the lignin biosynthetic pathway, and efforts to modify the quantity and quality of lignin have
already begun. Deployment of transgenic trees will face potential challenges on the regulatory and public
acceptance fronts. Trees present unique concerns, such as large scale and widespread pollen dispersal,
along with perennial persistence in the environment. For instance, certain plantation tree species are grown
in their native habitat, and in these cases, the potential for gene flow of engineered traits from modified
trees into native stands is a real possibility. Several approaches have been taken to mitigate gene flow.
This session will present speakers who will address the potential and challenges associated applying
biotechnology to tree improvement.
10:30
Introduction (T. Jones and M. Hinchee)
10:45
P-7
Gene Flow Control in Trees: Technology Development in Transgenic Poplars
Steven H. Strauss, Oregon State University
11:15
P-8
The Populus Genome: Development of the Information Resource
Gerald A. Tuskan, Oak Ridge National Lab
11:45
P-9
Genetic Engineering of Wood Formation in Forest Trees
Vincent L. Chiang, North Carolina State University
1:30 pm – 2:30 pm
Non-interactive Odd Poster Authors will be present.........Grand Ballroom
Interactive Poster Authors listed below will be present during their sessions
Moderator:
Nancy A. Reichert, Mississippi State University
1:30 pm – 2:30 pm
Interactive Plant Poster Session.......Grand Ballroom
P-2005
Kanamycin Resistant Alfalfa Has a Point Mutation in the 16S Plastid rRNA
Pierluigi Barone, University of Perugia, Italy, D. Rosellini, P. LaFayette, F. Veronesi,
and W. A. Parrott
P-2006
An Approach for Fiber Improvement in Kenaf Using a Gibberellin Oxidase Gene
Margaret M. Young, Mississippi State University, and N. A. Reichert
P-2007
A Poplar Promoter Functions in Potato and is Induced by Wounding and Fungal
Infection
Dmytro P. Yevtushenko, University of Victoria, Rafael Romero, William W. Kay, and
Santosh Misra
P-2008
Screening Arabidopsis thaliana and Chlamydomonas reinhardtii for Their Phenotypic
Response to 2,4,6-trinitrotoluene (TNT)
Nrupali Patel, University of Tennessee at Knoxville, and C. Neal Stewart, Jr.
P-2009
DNA Fingerprinting of Orchardgrass (Dactylis glomerata L.) cv. Persist for Plant Variety
Protection
Judith K. McDaniel, University of Tennessee, and B. V. Conger
P-2009A
Reactivation of a Disabled Virus Vector by the Utilization of an Inducible Recombination
System in Tobacco Plants
Arianne Tremblay, Federal Government of Canada, C. Beauchemin, V. Bougie, A.
Séguin, and J.-F. Laliberté
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IN VITRO TOOLS/EVALUATION
OF TOXICITY
Moderator:
Bobbie L. Thompson, HyClone Laboratories
1:30 pm – 2:30 pm
Joint Interactive Vertebrate/Toxicology Poster Session...Grand Ballroom
VT-2008
Design and Phase Ia Results of a Validation Study to Evaluate In Vitro Cytotoxicity
Assays for Predicting Rodent and Human Acute Systemic Toxicity
Michael W. Paris, NIEHS, J. A. Strickland, W. S. Stokes, S. Casati, A. P. Worth, H.
Raabe, C. Cao, R. Clothier, J. Harbell, R. Curren, J. Haseman, R. R. Tice, M. L. Wenk,
M. K. Vallant, G. Mun, M. Clear, G. O. Moyer, J. Madren-Whalley, C. Krishna, M. Owen,
and N. Bourne
VT-2009
The EpiOcular Prediction Model: In Vivo Versus In Vitro Draize Scores for Consumer
Products
George L. DeGeorge, MB Research Laboratories, M. Klausner, M. Osborn, K.
Bellavance, B. Breyfogle, J. Kubilus, and D. R. Cerven
VT-2010
Enhanced Phototoxicity Assay in Reconstituted Skin (EPARS)
George L. DeGeorge, MB Research Laboratories, A. C. Gilotti, T. L. Ripper, T. L. Fox,
L. Wagner, L. Lewis, S. H. Young, C. A. Kirk, M. K. Reeder, and B. Jones
VT-2011
Serum Alternatives to the Use of Fetal Bovine Serum in Cell Culture
Bobbie L. Thompson, HyClone Laboratories, B. Fujimoto, and B. Barnett
VT-2012
Toxic Effects of Organic and Inorganic Forms of Selenium in Murine Erytholeukemia and
Human Prostate Cancer Cells
Shyamal K. Majumdar, Lafayette College, S. A. Satuh, J. H. Tchaicha-Pavlic, and E. A.
Lucisano
VT-2013
Metalloprotease Inhibitors, Non-microbial Chemically Modified Tetracyclines and
Ilomastat, Block Anthrax, Lethal Factor Activity In Vitro
Salih Silay Kocer, State University of New York at Stony Brook, and Sanford Simon
VT-2014
The Effects of Glutamine and Ammonia Concentrations on CHO Cells in Serum-free
Media
Paula N. Decaria, HyClone Laboratories, Inc., J. Camire, and B. Barnett
Moderator:
Y. Judy Zhu, Hawaii Agriculture Research Center
Zhiwu Li, Kansas State University
3:00 pm – 5:00 pm Plant Contributed Paper Session.............
Clackamas
3:00
P-1015
Heterogeneity in Terpenoid Gene Expression in Transformed Roots of Artemisia annua
L. Grown in Bioreactors
Pamela J. Weathers,Worcester Polytechnic Institute, F. F. Souret, Y. J. Kim, B. E.
Wyslouzil, and K. K. Wobbe
3:15
P-1016
Utilization of RNA Interference to Confer Resistance to the Soybean Cyst Nematode,
Heterodera glycines
Ryan Matthew Steeves, Kansas State University, Timothy C. Todd, and Harold N. Trick
3:30
P-1017
Genetic Transformation and Plant Regeneration in Stevia rebaudiana Using
Microprojectile Bombardment
Kaye M. Knowles, Fort Valley State University, Seema Dhir, M. Singh, and Sarwan Dhir
3:45
P-1018
Shoot Formation of Agrobacterium Co-cultivated Tissues of Teak
Sri N. Widiyanto, Institut Teknologi Bandung, H. Rahmania, and S. Suhandono
4:00
P-1019
Towards a Mucosal Vaccine Against HIV
Nobuyuki Matoba, Arizona State University, M. Bomsel, C. J. Arntzen, and T. S. Mor
4:15
P-1020
Metabolic Engineering Phytoalexins from the Vitaceae Improves Antifungal Activity in
Tropical Plants
Y. Judy Zhu, Hawaii Agriculture Research Center, C. S. Tang, and P. H. Moore
4:30
P-1021
A Novel Protocol for Regeneration of Soybean from Mature and Immature Cotyledon;
Suitable for Genetic Transformation
Gregory Franklin, University of Toledo, E. Davis, S. Ismail, L. Carpenter, A. Hampton,
B. Smith, S. Ibeji, J. McDougall, B. Sadia, M. Parani, S. L. Goldman, and R. V. Sairam
Convener:
David D. Songstad, Monsanto Company
3:00 pm – 5:00 pm
Plant Symposium...............Washington/Clark
The influence of growth regulators on plant tissue culture and biotechnology is profound. The routine
manipulation of cells in vitro was possible only through the use of specific plant growth regulators to
promote either undifferentiated cell division or the formation of plants via organogenesis or embryogenesis.
In this symposium, the speakers will cover three plant growth regulators, abscisic acid, gibberellins and
ethylene, as they relate to in vitro culture and regenerated plants.
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3:00
Introduction (D. Songstad)
3:15
P-10
Persistent Abscisic Acid Analogs
Sue R. Abrams, Plant Biotechnology Institute at National Research Council of Canada
3:45
P-11
Gibberellins, Their Effects on and Roles in Growth and Differentiation of In Vitro
Cultured Plant Tissues, Organs, and Somatic Embryos
Richard Persons Pharis, University of Calgary
4:15
P-12
Ethylene Based Opportunities in Horticultural Biotechnology
Franzine D. Smith, The Scotts Company
IN VITRO QUANTITATION OF
ANGIOGENESIS: A BD BIOSCIENCES WORKSHOP
Convener:
Steve Ilsely, BD Biosciences Discovery Labware
3:00 pm – 5:00 pm
Animal Workshop...........................
Timberline
Angiogenesis, the formation of new blood vessels, is essential for normal growth and homeostasis. Certain
disease states can be exacerbated by the loss of tight control of angiogenesis, which results in either
excessive or insufficient blood vessel formation. The modulation of angiogenesis as a therapeutic strategy
is a rapidly expanding field for the drug discovery and research scientist. Several in vitro assays have been
developed to identify potential therapeutic molecules and to understand the mechanisms of angiogenesis.
However, because most of these assays are cumbersome, laborious, poorly quantitative, and lack
standardization, rapid progress in screening for effective therapeutic agents has been hampered. BD
Biosciences Discovery Labware has developed a portfolio of products designed to provide the scientist
easy, quick, robust, reproducible, standardized and readily available assay platforms for quantifying the
effects of angiogenesis modulating compounds. These assay products recapitulate one or more of the
neoangiogenic processes such as endothelial cell migration, invasion, and differentiation into vessels. The
use of these products for the in vitro quantitation of angiogenesis will be presented.
3:00
Introduction (S. Ilsley)
Panelists:
Stephen Ilsley, BD Biosciences Discovery Labware
Min Wu, BD Biosciences Discovery Labware
James Maliakal, BD Biosciences Discovery Labware
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