TUESDAY, JUNE 9
NATIONAL AND
INTERNATIONAL REGULATORY ASPECTS OF GEOS
Conveners:
John M. Cordts,
USDA/APHIS, and Thomas J. Flynn, US FDA
8:00
am – 10:00 am Plenary
Symposium Ballroom
A
Numerous genetically engineered organisms, both plant
and animal, have been developed over the last 30+ years for use in widely
variable applications. While many countries and regions around the world have
embraced the use of genetically engineered organisms, others continue to
wrestle with issues of food safety, environmental risk, ownership and patenting
of seed resources, and a variety of socio-economic changes. Speakers in this
session will explore on-going trends in regulatory agencies and potential
implications for international research and trade.
8:00 Introduction
(J. M. Cordts and T. J. Flynn)
8:05 PS-10 Interaction
of Global Regulatory Approaches and Testing Methodology with Trade
Raymond
D. Shillito, Bayer CropScience LP
8:40 PS-11 Safety
Assessment of Plant-incorporated Protectants (PIPs)
by the United States Environmental Protection Agency
John
L. Kough, US-EPA
9:15 PS-12 Animal
Clones and their Progeny, FDA’s GE Animal Guidance, and Other Recent Actions
Jeffrey Jones, FDA
9:50 Discussion
10:00
am – 10:30 am Coffee
Break Ballroom
B&C
IMPACT OF CELL
CONTAMINATION ON RESEARCH
Conveners:
John W. Harbell,
Mary Kay, Inc., and Tohru Masui, National Institute
of Biomedical Innovation, Japan
10:30
am – 12:30 pm Animal
Symposium Meeting
Room 1
In 2007, a group of leading cell culture scientists
petitioned the United States Secretary of Health and Human Services to develop
an active program for cell line authentication. They cited the importance of
cell cultures as research and teaching tools in diverse fields such as
medicine, genetics, drug discovery, reconstructive medicine, vaccine
development and basic research. Despite the importance of these tools, there
exists a gap in the fundamental principles of cell line quality control across
too much of the user community. In particular, they pointed to the documented
problems with cell line authentication. The frequency that research has been
compromised by the use of contaminated or misidentified cell lines has become a
clear concern to scientists, granting agencies and scientific journals. This is
not a new issue for in vitro research or the SIVB. Over three decades ago, Dr.
Nelson-Rees documented the contamination of many cell lines with the rapidly
growing HeLa line. Since then, other individuals and
organization have worked to identify problem cell lines and develop methods for
authentication. This symposium was organized to bring speakers from three of
the major cell repositories in Asia, Europe, and North America. Their
presentations will address the extent of the problem in each area, their
technical approaches for authentication, and overall programs to reduce the use
of misidentified cell line. While this symposium will focus largely on
animal-derived cells lines, the principles would be applicable to all cell
types.
10:30 Introduction (J. W. Harbell and T. Masui)
10:35 A-15 Online
Verification of Cell Line Authenticity Using an International Reference
Database
Wilhelm G. Dirks, DSMZ
11:10 A-16 Misidentification
of Animal Cell Lines: Impact on Research
Yvonne A. Reid, ATCC
11:45 A-17 Simple
Sequence Length Polymorphism (SSLP) Analysis to Determine the Strain from Which
Mouse Cell Lines are Derived
Yukio Nakamura, RIKEN BioResource Center
12:20 Discussion
INTELLIGENT MEDIA DESIGN
Conveners: Jean L. Roberts, Dow Agrosciences,
LLC, and Soverin Karmiol, SAFC BioSciences
10:30
am – 12:30 pm Joint
Symposium Ballroom
C1&C2
Culture
media and the physical environment cannot be overlooked as an influence in
tissue culture experiments. The utilization of nutrients in media by the cells
over the culture cycle causes physicochemical changes (pH, osmolarity)
in the medium; nutrient depletion can induce different metabolic pathways in
the cells during different culture phases, and the accumulation of toxins and
free radicals can induce cell death. We
will describe these changes and demonstrate how media optimization addresses
these potential sources of stress in cultured cells. The session includes presentations from the
plant and animal tissue culture communities.
10:30 Introduction
(J. L. Roberts and S. Karmiol)
Paul
J. Price, D-Finitive
Cell Technologies
11:00 J-5 A
Systematic Approach to Media Development with an Emphasis on Optimization at
the Basal Component Level
Soverin Karmiol, SAFC Biosciences
11:25 J-6 Medium
Changes During the Culture Cycle Influence Tobacco Suspension Cell Physiology
Jean L. Roberts, Dow AgroSciences
11:50 J-7 Investigation
of the Metabolism of Nicotiana tabacum Cells Using the Respiratory Monitoring System
(RAMOS)
David
A. Ullisch,
RWTH Aachen University
12:15 Discussion
PRODUCTION OF VALUE ADDED PRODUCTS IN TRANGENIC PLANTS
Convener: Maria N. Somleva,
Metabolix, Inc.
10:30
am – 12:30 pm Plant
Symposium Ballroom
A
As renewable sources of energy and raw materials,
agricultural feedstocks are the ultimate replacement
of declining oil reserves. Plants use solar energy and carbon dioxide to make
thousands of complex and functional biochemicals
beyond the capability of the modern synthetic chemistry. Plant-based products
include fine and bulk chemicals, pharmaceuticals, polymers, resins, food
additives, bio-colorants, adhesives, solvents, and lubricants. Plant inputs are
already cost-competitive for some of these products and have great social and
environmental benefits. Genetic engineering of crop plants as production
platforms for modified and novel materials provides a sustainable solution for
high value eco-friendly industrial applications. This approach requires new science
and technology to be directed at better understanding and modifying plant
development and metabolism, designing new harvesting and extraction
technologies, and developing improved manufacturing systems. The speakers in
this session will highlight recent advances in engineering bioenergy
and industrial crops for production of biodegradable plastics, rubber, and
modified plant oils.
10:30 Introduction
(M. Somleva)
Jan
Jaworski,
Donald Danforth Plant Science Center
11:00 P-24 Manipulation
of Reserve Content in Cottonseeds to Influence Oil, Protein, and Fiber Content
Kent
D. Chapman,
University of North Texas
11:25 P-25 Engineering
Rubber Production in Plants
Maureen
C. Whalen,
ARS/USDA
11:50 P-26 Biomass
and Industrial Oilseed Crops as Platforms for Production of Biodegradable
Plastics
Maria
N. Somleva,
Metabolix, Inc.
12:15 Discussion
CELLULAR PATHOLOGY AND CELL LINE
ESTABLISHMENT & CHARACTERIZATION
Moderator: Raziel S.
Hakim, Howard University
1:30
pm – 2:30 pm Animal
Interactive Poster Session Ballroom
B&C
A-2014 In
Vitro Determination of Protective Antigen Stability in a Novel, Transdermal Anthrax Vaccine
Kevin
E. Knockenhauer, State University of New York - Stony Brook,
Katarzyna M. Sawicka,
Elizabeth J. Roemer, and Sanford R. Simon
A-2015 Temperature-induced
Morphological Changes and Microsporidia Growth in the
Ep-1 Cell Line
S.
Richelle Monaghan, University of Waterloo, N. C. Bols, and L. E. J. Lee
A-2016 Establishment
of a Monarch Butterfly (Danaus plexippus, Lepidoptera: Danaidae)
Cell Line and Its Susceptibility to Insect Viruses
James
J. Grasela, USDA/ARS, and Arthur H. McIntosh
A-2017 Immunocytochemistry and Image Analysis of Beta-catenin Redistribution in Normal Human Colon Cell Cultures
Treated with Disinfection By-products
Ernest Winkfield, US EPA, Mary Moyer,
and Anthony DeAngelo
A-2018 OLGA:
A Case of Mistaken ID
Lucy E. J. Lee, Wilfrid
Laurier University, William J. Martin, Andre W. Christie, Marc Frischer, Thomas Soin, Guy Smagghe, Heather Braid, and Robert Hanner
A-2019 Development
of an Aiptasia pallida
Cell-culture System to Study Cnidarian-Dinoflagellate
Symbiosis
Jan Denofrio, Stanford
University, and John Pringle
A-2020 Development
of a Liver Cell Line from Fathead Minnow, Pimephales
promelas, and Their Molecular and Biochemical
Characterization
Lucy E. J. Lee, Wilfrid
Laurier University, N. Vo, J. Werner, R. Weil, N. D. Denslow,
and R. D Law
CELLULAR MODELS – EXTRACELLULAR MATRIX, 3D
MODELS, AND CRYOPRESERVATION
Moderator: Lia H.
Campbell, Cell & Tissue Systems, Inc.
1:30
pm – 2:30 pm Animal
Interactive Poster Session Ballroom
B&C
A-2021 Comparative
Gene Expression Analysis of 1- and 2-Methylanthracene in Rat Liver Stem Cells
Joon-Suk Park, Michigan State University, P. Babica, J. E. Trosko, and B. L. Upham
A-2022 Collagen
Production by Normal Human Dermal Fibroblast Cells in Serum-free Media
Nicole
Napolitano, State University of New York – Stony Brook,
M. Monaghan, E. Roemer, and S. Simon
A-2023 Analysis
of Extracellular Matrix Synthesized by Human Bronchial Smooth Muscle Cells In Vitro
Sumaria Zamurrad, State University of New York - Stony Brook,
L. J. Crawford, S. R. Simon, and E. J. Roemer
A-2024 The
Use of Collagen Matrices for Control of MMP and Serine Proteinase
Activity in Chronic Wounds
Katelyn D. Fenwick, State
University of New York - Stony Brook, S. R. Simon, F. Daccueil, and E. J. Roemer
A-2025 Validating
MTS as an Alternative Viability Assay to MTT on the Human 3-D Tissue Models, EpiAirwayTM and EpiDermTM
Catherine R. Kavanagh, State University of
New York - Stony Brook, L. J. Crawford, S. R. Simon, and E. J. Roemer
A-2026 Limited
Success in the Long-term Culture and Maintenance of Function of Non-plateable Lots of Cryopreserved
Primary Human Hepatocytes on a 2-dimensional Surface
Thomas J. Flynn, US FDA, and M. C.
Garcia
A-2027 Evaluation
of Apoptosis During Static Storage of Pancreatic Islet
Cells
Lia H. Campbell, Cell & Tissue
Systems, Inc., A. Vazquez, Z. Chen, and K. G. M. Brockbank
GENETIC
TRANSFORMATION FOR CROP IMPROVEMENT
Moderator: Michael Spencer, Monsanto Company
1:30
pm – 2:30 pm Plant
Interactive Poster Session Ballroom
B&C
P-2010 Hypoallergenicity
of Transgenic Peanut (Arachis hypogea L.) Is Transmitted to the Progeny
Anthony
Ananga, Alabama A&M University, Koffi Konan, and Hortense Dodo
P-2011 Reducing
Lignin Content in Bahaigrass (Paspalum
notatum Flugge) by RNAi Suppression of 4-Coumarate-CoA Ligase
Walid M. Fouad, University of Florida-IFAS, L. Martin, W. Vermerris, and F. Altpeter
P-2012 Expression
Analysis and Functional Characterization of Rice Genes Encoding microRNA156s
and Their Application in Plant Genetic Improvement
Dayong Li, Clemson University,
Xue Liu, Qian Hu, Lihuang Zhu, and Hong Luo
P-2013 Biotechnology
for Castor Oil Production
Grace Chen, USDA/ARS
P-2014 Heterologous Expression of Arabidopsis H+-PPase Enhances Salt and Drought Tolerance in Transgenic
Creeping Bentgrass (Agrostis
stolonifera L.)
Zhigang Li,
Clemson University, Christian M. Baldwin, Qian Hu, Haibo Liu, and Hong Luo
Tuesday, June 9
Odd Poster Authors will be present
2:30 pm – 3:30 pm
EVALUATION OF HUMAN HEALTH ENHANCING
PRODUCTS THROUGH ANIMAL ASSAYS
Conveners: Nirmal Joshee, Fort Valley State University and Elizabeth J.
Roemer, State University of New York - Stony Brook
3:30
pm – 5:00 pm Joint
Symposium Ballroom
A
Botanical materials have long been a fertile source of
bioactive components for human health care.
There are at least 250,000 species of plants out of which more than one
thousand have been found to possess significant anticancer properties. Botanical extracts are frequently used in the
cosmeceutical industry, and are finding expanding
applications as anti-inflammatory and wound healing pharmaceuticals. This session will review recent advances in
understanding the mechanisms of action and structure-function relationships of
botanically derived agents at the molecular, cellular and physiological levels.
3:30 Introduction
(N. Joshee and E. J. Roemer)
3:35 J-8 Use
of Multiple In Vitro Assays to Study Pleiotropic Anti-Inflammatory and Anti-metastatic Actions
of Low Molecular Weight Natural Products of Botanical Origin
Sanford R. Simon, State University of
New York - Stony Brook
4:00 J-9 Anti-tumor Mechanisms of Various Scutellaria Extracts and Constituent Flavonoids
Prahlad Parajuli, Wayne State
University and Karmanos Cancer Institute
4:25 J-10 Plant
Extracts for Cosmeceutical Applications: Genetic
Profiling of Cell Responses
Melissa
Monaghan,
State University of New York - Stony Brook
4:50 Discussion
INSECT PATHOGEN
FIELD EXPLORATION AND IN VITRO PROPAGATION
Conveners: Guido F. Caputo, National Resources
Canada, and Pauline O. Lawrence, University of Florida – Gainesville
3:30
pm – 5:00 pm Animal
Roundtable Meeting
Room 3
Insects,
the most diverse, damaging, and adaptable of all species can be found in the
most critical of environments ranging from ocean bottoms to swamps, desserts
and forests. Viruses, fungi, bacteria, and protozoa, can kill insects and are
often incorporated into an integrated pest management control program.
These pathogenic microorganisms are naturally occurring in nature and can
be reproduced to working volumes in laboratories. This session will focus on
the latest molecular identification techniques and genetic engineering
protocols and procedures for locating, collecting and processing these
organisms. Discussion will include susceptible hosts for their in vitro
propagation and the regulatory agencies and their requirements before they can
be re-introduced back to the field as biological control agents.
3:30 Introduction
(G. F. Caputo and P. O. Lawrence)
3:35 A-18 Current
Trends on the Identification and Propagation of Entomopathogenic
Nematodes
S. Patricia Stock, University of
Arizona
3:40 A-19 In Vitro Culture of an Entomopoxvirus
from a Parasitic Wasp and its Potential to Control Tephritid
Fruit Flies
Pauline O. Lawrence, University of
Florida
3:45 A-20 Foreign Exploration of Potential Entomopathogen Agents for the Control of the Diamondback
Moth, Plutella xylostella: a Case Study
James Grasela, USDA/ARS/BCIRL
3:50 A-21 Baculoviruses as Effective Bio-control Agents of Forest
Pests
Guido
F. Caputo, Natural
Resources Canada
3:55 Discussion
PROTOCOLS FOR
CELL CHARACTERIZATION, IDENTIFICATION, AND CONTAMINATION TESTING
Convener: Amy M. Wright, CIBA Vision Corporation
3:30
pm – 5:00 pm Animal
Symposium Meeting
Room 1
Data
collected from in vitro tissue culture studies are used in the determination
and evaluation of novel products and cell responses in the drug, medical device
and biomedical industries. To insure that the data collected are
accurate, the scientist needs tools for cell culture identification, possible
background contamination, and cross culture contamination. Increased
scrutiny within the publishing establishment has begun to require that the
studies performed with cell culture systems are representative as such. This
symposium will cover areas of concern; Endotoxin or
bacterial contamination, cell culture identification, protein levels of cell
cultures or within assays for biomarker determination.
3:30 Introduction
(A. M. Wright)
3:35 A-22 Using the PTS (Portable Test System):
Hand-held Technology for Rapid Assessment of Microbial Contamination
Norman R. Wainwright, Charles River Laboratories
4:00 A-23 Cell
Line ID and Contamination Detection by Allele-specific Real-time TaqMan PCR Assays
Caifu Chen, Life Technologies
4:25 A-24 Mycoplasmal Contamination: Risk Reduction Strategies
and Diagnostic Methods
Jill Mariano, Bionique Testing
Laboratories
4:50 Discussion
RISK ASSESSMENT AND TRANSGENE CONTAINMENT IN
TRANSGENIC CROPS
Convener: C.
Neal Stewart, Jr., University of Tennessee
3:30
pm – 5:00 pm Plant
Symposium Ballroom
C1&C2
To
the surprise of many scientists in plant biotechnology, transgene
flow from crops to non-transgenic crops and wild relatives is still of deep
regulatory, environmental and consumer concern.
This symposium will critically examine the risks that might result from transgene flow in the forms of hybridization and
introgression. Case studies will be
presented. Among the more prominent
cases are those involving creeping bentgrass and
alfalfa, in which gene flow has been an important determinant in preventing
commercialization, as well as post-commercialization of introgression of transgenes from herbicide-tolerant canola. Therefore, it is clear that biocontainment technologies would be valuable assets toward
reducing transgene flow risks and inadvertent
admixture of transgenes in erstwhile nontransgenic crops. Fortunately, there are recent
important developments of containment biotechnologies for removing transgenes, inducing male sterility, and precision tools
for altering gene transmission in plants. The symposium will look back at past
situations, examine current questions, and look forward to future containment
of transgenes.
The latter will be especially important as we consider future
applications of biotechnology in the development of perennial plants for the
new bioeconomy.
3:30 Introduction
(C. N. Stewart)
3:35 P-27 Gene
Flow in a Model GM Crop: Canola
Suzanne I. Warwick, Agriculture and Agri-food Canada
4:00 P-28 Strategies for Gene Confinement in
Genetically Modified Perennial Plants Used for Biofuels
Albert
P. Kausch, University of Rhode Island
4:25 P-29 Seed
Targeted Gene Confinement Strategies
Melvin
J. Oliver,
University of Missouri
4:50 Discussion
5:00 pm – 5:30 pm SIVB
Business Meeting Meeting
Room 3
(All Members Are
Urged to Attend)
Student Award
Presentations
5:45 pm – 10:00 pm An
Evening at the Magnolia Plantation Magnolia Plantation
Admittance
by Advance Ticket Holders Only
