SUNDAY, JUNE 7
GENE
TARGETING
Conveners: Richard Heller, Old Dominion University,
and Tzvi Tzfira, University of Michigan
8:00
am 10:00 am Plenary
Symposium Ballroom
A
Gene targeting is one of the most sought-after
technologies for animal and plant improvement. Gene targeting can potentially
be achieved by homologous recombination or by non-homologous end joining,
leading to a variety of outcomes, ranging from gene replacement and gene repair
to site-specific integration of foreign DNA molecules and site-specific
mutagenesis. Speakers in this session will introduce the audience to the
biological barriers and to the biotechnological solutions for achieving genome
editing and gene transfer in higher eukaryotic cells.
8:00 Introduction (R. Heller and T.
Tzfira)
8:05 PS-1 Cytoplasmic
and Nuclear Trafficking of Plasmids During Gene Delivery
David Dean, University of
Rochester
8:30 PS-2 Gene
Delivery
Richard Heller, Old Dominion University
8:55 PS-3 Motivating
Gene Targeting: How an Understanding of the Basic Mechanisms and Regulation of
Recombination May Yet Lead to Efficient Gene Replacement in Higher Plants
Anne Britt, University of
Califorina-Davis
9:20 PS-4 Zinc
Finger Nuclease-mediated Gene Targeting
Joseph F.
Petolino, Dow AgroSciences
9:45 Discussion
10:00
am 10:30 am Coffee Break Ballroom
B&C
BIO/TISSUE
ENGINEERING
Conveners: Elizabeth J. Roemer, State University of
New York - Stony Brook, and Paul J. Price, D-Finitive Cell Technologies
10:30
am 12:00 pm Animal
Symposium Meeting
Room 1
Tissue
Engineering combines cells, matrices, support structures or scaffolds and a
suitable environment to produce proto-tissues for the understanding of
Biological Development, Toxicity Testing and Regenerative Medicine. The
use of support systems and stem or tissue cells offer unique opportunities for
the repair, enhancement or replacement of damaged or diseased tissues and
organs. This Symposium will look at the formation of proto-tissues
through the interdisciplinary efforts of engineering, cellular maturation and
the understanding of biological systems.
10:30 A-3 Introduction to Regenerative Medicine
Paul J. Price, D-Finitive Cell Technologies
10:45 A-1 Tissue Engineering and Ex Vivo Tissue
Test Systems
Karen
J. L. Burg, Clemson University
11:20 A-2 Injectable Hydrogels for Brain Tissue
Regeneration After Traumatic Brain Injury (TBI)
Ning
Zhang, Clemson University
and MUSCc
11:50 Discussion
STUDENT ORAL PRESENTATION COMPETITION OF
PLANT BIOTECHNOLOGY RESEARCH
Moderator:
Fredy Altpeter, University
of Florida-IFAS
Panel of Plant Biotechnology
Experts Evaluating the Contestants:
Kan Wang, Iowa State University; Vincent Wingate, Biolex Therapeutics;
and John Finer, Ohio State University
10:30
am 12:30 pm Plant
Contributed Paper Competition Ballroom
A
To support the Society's vision to
encourage education and scientific informational exchange and recognize
outstanding students, the Plant Biotechnology section of SIVB offers an Oral
Presentation Competition of Plant Biotechnology Research. Finalists were selected based on the quality
of the abstracts. A panel of judges will
evaluate the presentations. Criteria are experimental design, data analysis,
proper interpretation of the results, originality of the study, technical
difficulty, appearance of the presentation and ability of the student to
respond to questions. Winners will be presented with a certificate and a cash
award at the Plant Biotechnology Section's Business Meeting.
10:30 P-1000 Expressing the Gibberllin Catabolizing Enzyme AT-GA-ox1 in a Low-input Turfgrass (Paspalum notatum Flugge) Improves Turf Quality and Field Performance
Paula Lomba,
University of Florida-IFAS, K. Kenworthy, and F. Altpeter
10:54 P-1001 Genetic
Engineering of Turfgrass and Rice with Two Novel Antimicrobial Peptides for
Enhanced Disease Resistance
Man Zhou, Clemson University, Allision Cason, and Hong Luo
11:18 P-1003 Highly
Efficient Suppressor-dependent Protein Expression in Plants with a Foxtail
Mosaic Virus Vector
Zun Liu, Baylor University,
Christopher M. Kearney
11:42 P-1004 Regeneration
of Arachis paraguariensis Through Different Morphogenic Pathways
Olubunmi
O. Aina, University of Florida, K. H. Quesensberry, and M.
Gallo
12:06 P-1005 Exogenous
Tocopherol and Ascorbic Acid Improve In Vitro Recovery of Cryopreserved Rubus
Shoot Tips
Esther E. Uchendu, Oregon State University, and Barbara M. Reed
POST DOCTORAL ORAL PRESENTATION COMPETITION
OF
PLANT BIOTECHNOLOGY RESEARCH
Moderator:
Michael Bosela, Indiana University
Purdue University at Fort Wayne
Panel of Plant Biotechnology
Experts Evaluating the Contestants:
Paula
Pijut, USDA Forest Service; Vibha Srivastava,
University of Arkansas; and Randall P. Niedz, USDA-ARS
10:30
am 12:30 pm Plant
Contributed Paper Competition Ballroom
C1&C2
To support the Society's vision to
encourage education and scientific informational exchange and recognize
outstanding post docs, the Plant Biotechnology section of SIVB offers an Oral
Presentation Competition of Plant Biotechnology Research. Finalists were selected based on the quality
of the abstracts. A panel of judges will
evaluate the presentations. Criteria are experimental design, data analysis,
proper interpretation of the results, originality of the study, technical
difficulty, appearance of the presentation and ability of the student to
respond to questions. Winners will be presented with a certificate and a cash
award at the Plant Biotechnology Section's Business Meeting.
10:30 P-1006 Host-delivered RNAi: An Effective Strategy
to Silence Nematode Genes in Transgenic Hairy Roots of Soybean
Jiarui,
Li, Kansas State University, Timothy C. Todd, William T. Schapaugh,
and Harold N. Trick
10:50 P-1007 Higher
Accumulation of F1-V Fusion Recombinant Protein in Plants After Induction of
Protein Body Formation
Maria Lucrecia Alvarez, Arizona
State University, Emel Topal, Federico Martin, and Guy A. Cardineau
11:10 P-1008 Transposon
Mutagenesis of Soybean (Glycine max) Using the Rice MITE mPing
Charles
N. Hancock, University of Georgia, F.
Zhang, D. M. Tucker, S. R. Wessler, and W. A. Parrott
11:30 P-1009 TILLING for Peanut Improvement
Joseph
E. Knoll, University of Georgia, M. L. Ramos, and P. Ozias-Akins
11:50 P-1010 Integration
and Expression of E. coli L-aspartate-alpha-decarboxylase in Tobacco
Chloroplasts Enhances Photosynthesis and Biomass Accumulation Following High
Temperature Stress
Walid
M. Fouad, University of Florida-IFAS,
and Fredy Altpeter
12:10 P-1011 Using
Endogenous Vitis Genes to Produce Disease Resistant Transgenic
Grapevines
Sadanand
A. Dhekney, University of
Florida-IFAS, Z. T. Li, T. W. Zimmerman, and D. J. Gray
CELL CULTURE OF
MARINE INVERTEBRATES
Conveners: James J. Grasela, USDA ARS BCIRL; Lucy E.
J. Lee, Wilfrid Laurier University; and Guy Smagghe, Ghent University
1:30
pm 3:00 pm Animal
Symposium Meeting
Room 1
For
a number of years marine invertebrate cell culture research has lagged behind
other areas of invertebrate cell culture, particularly insects, in the
development of established cell culture lines. Indeed,
tremendous effort has been made to establish long-term (>1 month) continuous
invertebrate cell lines, although to date there
are no published reports of a successfully established marine invertebrate cell
culture. Nevertheless, many primary
marine invertebrate cell cultures have been developed for the purpose of
providing novel biologically active chemical compounds with pharmaceutical
properties. Furthermore, the study of
diseases from many valuable commercial species such as crayfish, lobsters,
shrimps, mussels, oysters, and clams has also been an incentive to develop
viable marine cell cultures. This
session will focus on the continued development
of marine invertebrate cell culture, the potential obstacles encountered in an
attempt to establish long-term marine cell cultures, and some of the new
technologies available that may help to overcome these problems.
1:30 Introduction
(L. E. J. Lee)
1:35 A-4 Transfection
of Marine Sponge Cells to Produce a Cell Line
Shirley A. Pomponi, Harbor Branch Oceanographic Institute
2:00 A-5 Stem
Cells from Marine Invertebrates Perspectives and Prospective
Baruch Rinkevich, National
Institute of Oceanography
2:25 A-6 Crustacean and Their Pathogens: Developing Susceptible Host
Cell Lines and Alternative Approaches
Karen G. Burnett, Grice Marine
Laboratory
2:50 Discussion
CRYOPRESERVATION
Conveners: David D. Ellis, USDA/ARS, and Lia H.
Campbell, Cell and Tissue Systems, Inc.
1:30
pm 3:00 pm Joint
Symposium Ballroom
C1&C2
The cryopreservation of living cells is becoming
routine with many cell, organ or tissue types in both plants and animals. Despite this, many cellular aspects of
cellular, biochemical, structural and molecular biology with respect to the
ability to survive cryopreservation are unknown even though many of these
aspects are likely to be common between animal and plant cells. We know that intracellular ice formation and
growth is lethal in most systems and that dehydration or removal of the water
from the cells and intercelluar spaces is often required. Yet cells require this aqueous environment to
survive. This symposium will explore
current state of the art in plant and animal cryobiology with an emphasis on
sustaining a viable, living cell capable of further growth and manipulation
when removed from liquid nitrogen.
1:30 Introduction (D. D. Ellis and
L. H. Campbell)
1:35 J-1 Storage and Distribution Issues for
Cryopreserved Cells and Tissues
Kelvin
G. M. Brockbank, Cell & Tissue Systems, Inc.
2:00 J-2 Preservation of Biomaterials in the Dry
State: Lessons from Nature
John H. Crowe, University of California-Davis
2:25 J-3 Beyond
the Cell Wall: Comparisons Between Plant and Animal Cell Cryopreservation
Hugh
Pritchard, UK Seed Conservation
2:50 Discussion
TRANSGENIC APPROACHES IN OIL MODIFICATION
Convener: T. E. Clemente, University of Nebraska
1:30
pm 3:00 pm Plant
Symposium Ballroom
A
A
significant amount of progress has been made in understanding fatty acid
biosynthesis in plant cells, in addition to a wealth of DNA sequence
information that is available from a plethora of organisms. These biochemical
and genomics resources will serve as valuable tools that will enable the
translation of this information to applied technologies. The plant kingdom possesses a large
repository of fatty acids with different structures and physical properties
that hold great potential as sources for food, biofuel and biomaterial
applications. However, many of these
novel fatty acids accumulate in plants that lack agronomic potential. Hence, the ability to manipulate oil
metabolism in scalable feedstocks through genetic transformation of established
lipid feedstocks offers a realistic avenue for the synthesis of novel fats at
production levels that can meet the demands of the market. The speakers in this session will describe
strategies for the modification of seed oils for various market
applications. A round-table discussion
will be held at the end of the session allowing for an open dialogue on the
promise and hurdles facing the development and marketing of modified feedstocks
for the production of novel oils for food, feed, and biomaterials.
1:30 Introduction
(T. E. Clemente)
1:35 P-1 Modifying
Soybean Oil for Feed and Fuel
T. E. Clemente, University of
Nebraska-Lincoln
2:00 P-2 Enhancing
Soybean Seed Lipid Biosynthesis for Nutritional, Energy, and Industrial
Applications
Anthony J. Kinney, DuPont Experimental Station
2:25 P-3 Camelina
sativa, a Potential Oilseed Platform for the Production of High-value
Industrial Oils
Edgar B. Cahoon, University of Nebraska-Lincoln
2:50 Discussion
2009 IN VITRO BIOLOGY
OPENING CEREMONY
Program
Chair: David D. Songstad,
Monsanto Company
3:00
pm 5:30 pm Opening
Ceremony Ballroom
A
Conveners: Todd
J. Jones, BASF
David
D. Songstad, Monsanto Company
3:00 Welcome and Opening Remarks:
Todd J. Jones,
President, Society for In Vitro Biology
3:05 ROBERT H. LAWRENCE, JR.
KEYNOTE SYMPOSIUM
Introduction
(T. J. Jones and D. D. Songstad)
KS-1 Introduction to the DOE BioEnergy Science
Center
Russ Miller, Manager of Technology
Transfer and Partnerships
4:00 Questions
and Answers
4:15 2009
SOCIETY FOR IN VITRO BIOLOGY AWARDS CEREMONY
4:20 2009
Young Scientist Award Recipient (Award to be presented at Section Meeting)
Tzvi
Tzfira, University of Michigan
2009
Fellow Award Recipients (Awards to be presented at Section Meetings)
Daniel C. W. Brown, Agriculture & Agri-Food Canada
Lia H. Campbell, Cell & Tissue Systems, Inc.
Michael E. Kane, University of Florida
Pamela J. Weathers, Worcester
Polytechnic Institute
4:25 2009 Distinguished Service
Award Presentations
(Awards to be presented
by Todd J. Jones):
John W. Harbell, Mary Kay Inc.
Peggy J. Ozias-Akins, University of
Georgia
Wayne A. Parrott, University of
Georgia
T. Michael Spencer, Monsanto Company
Kan Wang, Iowa State
University
2009 Lifetime
Achievement Award Presentations:
4:30 (Introduction by Lucy E. J.
Lee; Acknowledgement by Dr. Bayne to follow)
Christopher J. Bayne, Oregon State
University
4:45 (Introduction
by Gregory C. Phillips; Acknowledgement by Dr. Collins to follow)
Glenn B. Collins, University of Kentucky
5:00 (Introduction
by Chieri Kubota; Acknowledgement by Prof. Kozai to follow)
Toyoki Kozai, Chiba University
5:15 (Introduction
by Sandra L. Schneider and Tetsuji Okamoto; Acknowledgement by Prof. Namba to
follow)
Masayoshi Namba, Okayama
University Medical School
5:30 pm 6:30 pm 2009 IN VITRO BIOLOGY Ballroom B&C
OPENING CEREMONY RECEPTION
5:30
pm 7:00 pm IVACS Student Poster Competition Ballroom B&C
5:30
pm 7:30 pm Exhibits and Posters Ballroom B&C
Sunday, June 7
Odd Poster Authors will be present
6:30 pm 7:30 pm
STUDENT NETWORKING
SYMPOSIUM: BUILD YOUR WRITTEN/ORAL SKILLS, ADVERTISE YOURSELF, AND FIND THAT
PERFECT JOB
Convener: Phillip J. Kauth, University of Florida
7:30
pm 9:30 pm Education
Symposium Meeting
Room 3
Professional networking is easily the most effective and
powerful strategy for conducting a job search in any economy. In 2009, with the state of the current
economy and the dismal employment outlook, it may be the only way to identify
and locate available positions and help get a foot in the door. The primary
responsibilities of the job seeker in this process are to be educated about
their prospective audience, the current trends in their targeted field, and to
make sure he/she is equipped with the best possible tools and have them readily
available. After conducting some basic
research on where the job pools may exist for graduates with a background in
biology and research with this particular focus, there appears to be reason for
optimism. The areas of health care research, pharmaceuticals, biotechnology and
genetically driven research are not occupational areas included in the current
negative employment projections. There is every reason to believe that a well
prepared candidate, with an effective job search plan, will be successful in
securing a position in their chosen field.
7:30 Introduction
(P. J. Kauth)
7:35 E-1 Build
Your Written/Oral Skills, Advertise Yourself and Find That Perfect Job
Karen Eippert, College of
Charleston
8:00 Workshop
