MONDAY, JUNE 8
Conveners:
Sixue
Chen, University of Florida, and Jacqueline A. Jordan, Clayton State University
8:00 am – 10:00 am Plenary
Symposium Ballroom
A
Genomics,
Proteomics, and Metabolomics are the new pillars of
modern biological sciences. Systems
Biology integrates these three “omics” to study and
understand the entire living organism.
Conceptual and technical advancements in systems biology have created
numerous opportunities in research and this contributes to the understanding of
the complexities within biological systems.
As these technologies advance to common use, it is essential for
biologists to develop strategies to handle, analyze and integrate large
quantities of data. Speakers in this
session will introduce recent technological developments in different “omics” areas. Real
applications in data integration and elucidation of biological processes and
networks will be introduced. In this
session, future directions will be highlighted and key issues facing the area
of systems biology discussed.
8:00 Introduction
(S. Chen and J. A. Jordan)
8:05 PS-5 Introduction to Research in Systems Biology
Jacqueline A. Jordan, Clayton
State University
8:25 PS-6 Beyond
Gene Clustering of Microarray Data
W. Jim Zheng, Medical University
of South Carolina
8:45 PS-7 Integrating High-throughput and Genomics
Technologies in Least Expected Places
Maja Kodani, Centers for Disease Control and Prevention
9:05 PS-8 Metabolomics and
Integrated Functional Genomics Reveal Novel Information Related to Medicago Secondary Metabolism
Lloyd Sumner, Noble Foundation
9:25 PS-9 Towards a Systems
Analysis of Plant Molecular Networks
Sixue Chen, University of Florida
9:45 Discussion
10:00 am – 10:30 am Coffee
Break Ballroom
B&C
PLANT IN VITRO CULTURE LAB EXERCISES
Convener:
Michael E. Kane, University of
Florida
10:30 am – 12:00 pm Plant
Workshop Ballroom
C1&C2
College science
teachers often need new and exciting approaches to engage students during
laboratory sessions. In vitro biology exercises are easy to incorporate into
science courses. Many students have limited exposure to in vitro biology, yet
become very interested once exposed to various in vitro techniques. Including
in vitro techniques into laboratory curriculum is an exciting approach to teach
students critical thinking and basic laboratory skills. However, the
availability of reliable, effective, and efficient laboratory exercises is
often limited. During this symposium an overview of four unique in vitro plant
tissue culture laboratory exercises will be presented.
10:30 Introduction (M. E. Kane)
10:40 P-4 Using
Tobacco to Teach Plant Tissue Culture and Transformation
Margaret Young, Elizabeth City State University
11:00 P-5 A Classroom Exercise in Asymbiotic
Orchid Seed Germination: The Whole Story
Timothy R. Johnson and Philip Kauth,
University of Florida
11:20 P-6 Using
Plant Tissue Cultures to Demonstrate Mineral Nutrient Deficiencies: Comparison
with Conventional Hydroponic Techniques
Michael Bosela,
Indiana University- Purdue University at Fort Wayne
11:40 P-7 Synthetic
Seed Technology Demonstrated Through a Novel Teaching Exercise
Michael E. Kane, University of Florida
JUNE BRADLAW MEMORIAL SYMPOSIUM: TOXICOLOGY IN THE 21ST CENTURY
Conveners: Eugene Elmore, University of California –
Irvine, and Brad L. Upham, Michigan State University
10:30 am – 12:30 pm Animal
Symposium Meeting
Room 1
The current animal model systems for
predicting both toxicology and drug efficacy for human responses have been very
ineffective. This was evident from the
studies with cancer therapeutics where adverse events relating to critical
tissues were not adequately predicted by animal studies. The National Research Council published a
report in 2007 (http://dels.nas.edu/dels/rpt_briefs/Toxicity_Testing_final.pdf)
that established the vision and strategy to implement a paradigm shift from
classic animal models for predicting human toxicity and risk to a focus on in
vitro model systems that use human cell systems derived from different tissues
to identify toxicity pathways that would be more predictive of human
responses. The vision suggests that by
using the best current science, we would ultimately be able to identify toxic
agent mechanisms that would minimize or eliminate testing in animal
models. Full implementation of the
vision and strategy will require substantial funding, coordinated efforts, and
application of our best scientific approaches to understand the critical
pathways to toxicity. With the advances
in the various “omics” technologies progressing at a
rapid pace, the quest for better predictive models of human toxicology and
efficacy will speed up the practical application of these technologies in determining
human tissue specific responses to drugs and environmental agents. The completion of the human genome sequence
and the evolution of the tools for analysis of gene expression, proteomics, and
metabolomics have resulted in the generation of massive
data sets on relevant pathways and molecular biology. This “explosion” of information in
identifying new genes and gene interactions has created a major challenge in
the biologically meaningful interpretation of this data. Bioinformatics tools are also rapidly
evolving to assist in making biologically relevant interpretations of these
complex data. Understanding agent induced pathway perturbations will also
result in a better understanding of efficacy pathways. The outcome of the coordinated efforts will
result in improved approaches to personalized medicine and reduced
environmental risk. This session will
focus on current and future approaches to implement the “vision and strategy”
with a focus on human cell model systems, “omics”,
and bioinformatics.
10:30 Introduction (E. Elmore and B.
L. Upham)
10:40 A-7 Toxicity Pathways, In Vitro Assays, and
Computational Cell Biology: Using the Best Science for Toxicity Testing
and Risk Assessment
Melvin E.
Andersen, The Hamner Institutes for Health
Sciences
11:05 A-8 Mechanistic Insight into Drug-induced
Organ Injury with Comparisons of Animal and Human Tissue
Alison E. M. Vickers, Allergan
11:30 A-9 Knowledge Profile Aporoach:
Insights Into Drug Action and Toxicity Mechanisms
Nikolai
Daraselia, Ariadne Genomics
11:55 A-10 Using Gene Expression and Pathway Analysis
for Efficacy and Toxicity Assessment
Eugene Elmore,
University of California - Irvine
12:20 Discussion
TREE
BIOTECHNOLOGY
Conveners: Shujun Chang, ArborGen, LLC, and Scott Merkle,
University of Georgia
10:30 am – 12:30 pm Plant
Symposium Ballroom
A
Forest trees are
important resources for the world. As forested land area continues to decline
at the rate of about 7.7 million ha/year due mainly to conversion to
agriculture or urbanization, purpose-grown, highly-productive forest
plantations have become an increasingly important source of wood and other
forest products. Building upon the gains made via conventional breeding and silvicultural
practices, biotechnology offers an exciting opportunity to optimize the
potential of trees and to bring quality and productivity to an unprecedented
level. In this session, we intend to
highlight recent, exciting developments in tree biotechnology. Speakers will present their research
demonstrating recent developments in tree genomics, gene discovery, and gene
function analysis and product development through mass clonal
propagation and genetic transformation.
10:30 Introduction
(S. Chang and S. Merkle)
Maud Hinchee, ArborGen LLC
10:55 P-9 Populus Genomics, Candidate Gene Identification and
Accelerated Domestication
Gerald A. Tuskan,
Oak Ridge National Lab
11:15 P-10 Cisgenic and Intragenic Approaches to Genetic Modification of Growth and
Form in Poplar
Steven Strauss, Oregon State University
11:35 P-11 Phenylpropanoid
Networking in Populus
Chung-Jui Tsai, University of Georgia
11:55 P-12 Hardwood Tree Biotech Advances in
Southeast
Scott Merkle, University of
Georgia
12:15 Discussion
STUDENT
NETWORKING LUNCHEON
Conveners:
Phillip J. Kauth,
University of Florida, and S. Richelle Monaghan,
University of Waterloo
12:30 pm – 2:00 pm Education
Symposium Meeting
Rooms 8&9
Young
scientists and their advisors are invited to attend this luncheon to discuss
what they learned from Saturday and Sunday’s workshops. This will also be
an excellent opportunity for attendees to further network with the speakers and
mock interviewers from Sunday night. A final goal is to determine what
other areas of career development are necessary to the young SIVB scientist and
could be focused on for the 2009 Student Committee hosted session.
12:30 Introduction (P. J. Kauth and S. R. Monaghan)
12:40 E-2 A Government
Scientist: How It Works with the Agricultural Research Service
David Ellis, USDA/ARS
CANCER BIOLOGY AND CELL-CELL INTERACTIONS
Moderator: Brad
L. Upham, Michigan State University
1:30 pm – 2:30 pm Animal Interactive
Poster Session Ballroom
B&C
A-2000 The
Role of Various Phospholipase A2 Enzymes
in Key Cell Signaling Events Affiliated with Tumor Promotion
Brad
L. Upham, Michigan State University, J. S.
Park, P. Babica, O. Adamovsky,
and J. E. Trosko
A-2001 The
Established Insect Cell Line, BCIRL-HzAM1, Expresses a Cellular and a Secretory Phospholipase A2
Cynthia
L. Goodman, USDA/ARS/BCIRL, and D. Stanley
A-2002 Tumor
Promotion-relevant Cell Signaling: Key Roles of Annexins
and Phospholipases
Pavel Babica, Michigan State University, J. S. Park, I. Sovadinova, L. Blaha, D. A.
Whitten, C. G. Wilkerson, J. E. Trosko, and B. L. Upham
A-2003 Differential
Expression of Full-length and Truncated Cullin-5 is Associated with
Granulocytic Differentiation of HL-60 Leukemia Cells
Michael
J. Fay, Midwestern University, G. K. Tan, S. S. Baxter, M. L. Hall,
A. M. S. Mayer, and L. A. Carlson
A-2004 Effects
of 5-Fluorouracil Treatment on MicroRNA Expression in
MCF-7 Breast Cancer Cells
Maitri Shah, East Carolina
University, Mary A. Farwell, and Baohong Zhang
A-2005 The
Effect of Green Tea Polyphenon 60 Treatment on miRNA Expression in MCF7 Breast Cancer Cells
Lindsey
N. Fix, East Carolina University,Mary A. Farwell, and Baohong
Zhang
A-2006 Development
of Organ Culture Conditions for Maintenance of Normal and Malignant Human Colon
Tissue
Narasimharao V. Bhagavathula, University of Michigan, M. K.
Dame, M. N. Aslam, and J. Varani
CELLULAR MODELS – POLICY, IMMUNITY, AND TOXICOLOGY
Moderator: Elizabeth
J. Roemer, State University of New York - Stony Brook
1:30 pm – 2:30 pm Animal Interactive Poster
Session Ballroom
B&C
A-2007 The
Canadian Council on Animal Care: Replacement, Reduction, Refinement
Alternatives Online
Gilly Griffin,
Canadian Council on Animal Care, and Nicole Fenwick
A-2008 Efficacy Testing of a Novel
Retinoid in Gottingen Mini-pig Skin Organ Culture Yields Comparable Results to
That in Intact Animal
Michael
K. Dame, The University of
Michigan Medical School, T. Paruchuri, M. DaSilva, N. Bhagavathula, W. Ridder, and J. Varani
A-2009 Healing of Dermal Wounds in the EpiDerm-FT In Vitro Human Skin
Model
Patrick
Hayden, MatTek
Corporation, G. Stolper, C. Cooney, and M. Klausner
A-2010 Use of Cultured Human Skin Cells
and a Skin Tissue Construct as Models to Study the Genotoxic
Marker γ-H2AX Following
Sulfur Mustard Exposure
A.
L. Miller, United States Army
Medical Research Institute of Chemical Defense, C. L. Gross, E. W. Nealley, O. E. Clark, N. K. Waraich,
K. L. Rodgers, and W. J. Smith
A-2011 Human
Bronchial Epithelial Cells as a Model for Human Lung Airway Injury and Therapeutic
Intervention Following Sulfur Mustard Exposure In
Vitro
O.
E. Clark, United States Army
Medical Research Institute of Chemical Defense, E. W. Nealley,
A. L. Miller, Y.-S. Jung, and W. J. Smith
A-2012 Lack of Genotoxicity
in Cultured Human Small Airway and Microvascular
Cells Following Exposure to the Nerve Agent, VX
C. L. Gross,
United States Army Medical Research Institute of Chemical Defense, E. W. Nealley, A. L. Miller, M. T. Nipwoda,
O. E. Clark, and W. J. Smith
A-2013 Comparison
of Fish Cell Line Responses to Chemicals and Process-affected Waters from the
Oil Sands of Alberta, Canada
Bryan
Sansom,
University of Waterloo, R. Kavanagh, M. MacKinnon, D.
G. Dixon, and L. E. J. Lee
IN
VITRO BIOLOGY
Moderator: Michael
Spencer, Monsanto Company
1:30 pm – 2:30 pm Plant Interactive
Poster Session Ballroom
B&C
P-2000 Low-cost Methods for Production of
Commercial-size Fruit from Sugar-loaf Pineapple (Anasas
comosus) Plantlets in Jamaica
Sylvia A. Mitchell, University of the West Indies, M. H. Ahmad
P-2001 Macronutrient
Optimization for In Vitro Growth of Turmeric (Curcurma
longa L.)
Sean Michael
Halloran, Clemson University,
and Jeffrey Adelberg
P-2002 Secondary Metabolism Inducing
Treatments During In Vitro Development of Turmeric (Curcuma
longa L.) Rhizomes
Matthew M. Cousins, Clemson University, Jeffrey Adelberg,
Feng Chen and James Reick
P-2003 The Use of Silver Compounds to
Direct Plant Tissue Culture Development
Benjamin Steinitz,
The Volcani Center, Y. Tabib, N. Bar, and N. Bernstein
P-2004 Obtaining Sethoxydim
Resistance in Seashore Paspalum (Paspalum
vaginatum) Via In Vitro
Selection
Douglas L. Heckart, University of Georgia, W. Parrot, and P. Raymer
TRANSFORMATION
TECHNOLOGY
Moderator: Lisa
Lee, The Scotts Miracle-Gro
Company
1:30 pm – 2:30 pm Plant Interactive
Poster Session Ballroom
B&C
P-2005 Rice Promoters That Confer Organ-specific
Transgene Expression
Roger Thilmony,
USDA/ARS Western Regional Research Center, Mara E. Guttman,
Meridith Cook, and James G. Thomson
P-2006 Identification of Parameters
Influencing Agrobacterium-mediated
Transformation of Peanut
N'NAn A. S. Diby, Alabama A&M University, K. Konan, and H. Dodo
P-2007 Comparison Between
Mannose and Kanamycin Selection Systems for Genetic
Transformation of Citrus
Manjul Dutt, University of Florida-IFAS, and J. W. Grosser
P-2008 Site-specific Excision of Targeted
DNA Is Facilitated by Bxb1 in the Arabidopsis Genome
James Thomson, USDA Western Regional Center, Y. Y. Yau,
R. Blanvillain, and D. Ow
P-2009 Induction of nptII
Exogene Removal from Transgenic Grape (Vitis vinifera ‘Brachetto’
Lucia Martinelli,
IASMA Research Center, L. Dalla Costa, I. Vaccari, V. Poletti, and M. Mandolini
Monday, June
8
Even Poster
Authors will be present
2:30 pm –
3:30 pm
CHALLENGES
TO LARGE SCALE LIQUID PLANT MICROPROPAGATION
Conveners: Jeffrey
W. Adelberg, Clemson University and Ebrahim Firoozabady, Del Monte
Fresh Produce Company
3:30 pm – 5:00 pm Plant
Symposium Meeting
Rooms 8&9
Micropropagation is often limited to high value ornamental
plants and found too costly for large-scale industrial crops. Almost all micropropagation is done on semi-solid medium despite
greater efficiencies of liquid media propagation: lower materials cost and less
preparation time, reduced labor during transfer - clean room and greenhouse,
greater plant growth due to better uptake of nutrients, and greater
opportunities for automation in scale-up. Considerable barriers, however,
may prevent implementation of liquid culture in large scale operations.
These include the possibility of hyperhydricity,
a lack of equipment and process, the increased risk of contamination in large
vessels, and costly investment in new vessels with mechanized aeration.
In this symposium, speakers will share their experiences developing
commercially scaled liquid micropropagation systems
with value added germplasm.
3:30 Introduction
(J. W. Adelberg)
3:35 P-13 Introduction
to Large Scale Liquid Micropropagation Systems
Jeffrey W. Adelberg, Clemson
University
3:55 P-14 Transgenic
Loblolly Pine and the Importance of Somatic Embryogenesis Scale-up Technologies
for Future Commercialization
Shujun
Chang, ArborGen, LLC
4:15 P-15 Challenges
to Large Scale Liquid Plant Micropropagation – the Arundo Story
Laszlo
Marton, University
of South Carolina
4:35 P-16 The
Utilization of Temporary Immersion System (RITA) to Enhance Initial Steps in
Commercial Micropropagation of Cordyline spp.
Cuauhtemoc Navarro, Agromod
4:55 Discussion
COMMERCIALLY
AVAILABLE IN VITRO 3-D TISSUE MODELS FOR BIOMEDICAL RESEARCH AND PRODUCT
DEVELOPMENT
Convener: Elizabeth
J. Roemer, State University of New York - Stony Brook
3:30 pm – 5:00 pm Animal
Symposium Meeting
Room 1
Cells inhabit
three-dimensional (3-D) space when tissues are growing in vivo. When cells are grown in vitro as 3-D
constructs they better mimic in vivo tissue.
3-D models can be manipulated through the use of multiple cell types,
matrices, and culture conditions to yield constructs with histological and
physiological profiles closely matched to those found in the body. Through recreation of complex mechanical and
biochemical microenvironments, these systems are proving increasingly valuable
for multiple applications including studies of pathological and normal
physiological processes, pre-clinical drug development and in vitro
toxicology. 3-D models are also key
alternatives to animal testing. While it
is possible to grow 3-D models in the research lab, the option of purchasing
ready-to-use constructs is often attractive.
This workshop will explore a range of commercially available models.
3:30 Introduction
(E. J. Roemer)
3:35 A-11 3-D
Tissue Models in Contract Research: Points to Consider for Efficacy, Product
Development, or Regulatory Testing Programs.
Hans Raabe,
Institute for In
Vitro Sciences, Inc.
4:00 A-12 Customized
3D Human Tissues for Discovery
Christophe Egles, Tufts
University School of Dental Medicine
4:25 A-13 Commercial
Production of In Vitro Human Epithelial Models at MatTek
Corporation: A Survey of Available Models and Applications
Patrick
Hayden, MatTek Corporation
4:50 Discussion
PLANT TRANSGENE EXPRESSION SYSTEMS
Convener:
Allan R. Wenck,
BASF Plant Systems, LLC
3:30 pm – 5:00 pm Plant
Symposium Ballroom
A
Technology is
rapidly evolving to deliver improved and alternative methods for currently
utilized plant transgene expression systems. One gene may no longer be enough. In addition, methods to fine-tune expression
of transgenes and even endogenous genes are
required. Further, multi-gene solutions
and even whole metabolic pathways are increasingly being seen as required for
transgenic solutions. In this session
several different possibilities will be explored. New modular vectors for Agrobacterium
mediated transformation will be discussed.
Technology to better control transgenes and
even endogenous genes will be presented.
Finally, the potential for engineering complete plant artificial
chromosomes will be explored.
3:30 P-17 Modular
Assembly of Multi Gene Plant Transformation Vectors
Tzvi
Tzfira,
University of Michigan
4:00 P-18 Editing the Genome of Crop Plants with Engineered Zinc
Finger Proteins
Philip
D. Gregory, Sangamo BioSciences
4:30 P-19 Engineered
Minichromosomes in Maize
James A. Birchler, University of Missouri
BIOASSAY PROTOCOLS
Convener:
Dennis A. Laska,
Eli Lilly & Company
5:00 pm – 6:00 pm Animal
Workshop Meeting
Room 1
Development and
validation of reliable bioassays is critical in the consumer product and
pharmaceutical industries, as well as for environmental monitoring and
bio-defense. This workshop will evaluate
the basic requirements and expectations of bioassay development in a round
table discussion following a featured presentation by Dr. Pamela Morris and Ms.
Maria Vizcaino from the Hollings Marine Laboratory (Charleston, SC).
5:00 Introduction (D. A. Laska)
5:05 A-14 Determining
the Antimicrobial Activity of Bacteria Associated with a Caribbean Coral
Pamela J. Morris
and Maria I. Vizcaino, Hollings Marine Laboratory
5:45 Panel
Discussion
Panelists:
John W. Harbell, Mary Kay Corporation
William J. Smith, US Army Medical
Research Inst of Chem Defense
Amy A. Wang,
GlaxoSmithKline
GRAPHICAL AND STATISTICAL SOFTWARE AND ANIMATED
VISUALIZATIONS
Convener:
Jeffrey W. Adelberg,
Clemson University
5:00 pm – 6:00 pm Plant
Workshop Ballroom
A
Graphical visualization of data from
multivariate analyses allows us to explore the statistical,
and perhaps cryptic relationships, among treatments. The workshop will 1) show strategies and
methods for designing in vitro experimentation where multiple factors (e.g.,
media inputs and environmental conditions) and responses (e.g., growth and
development) are varied; 2) analyze with the objective of optimizing one or
multiple responses; and 3) present the data analysis in highly graphical
formats (static and dynamic) that capture the most important factor/response
relationships. The overall approach is
commonly used in process engineering, but has particular relevance to in vitro
biology experimentation.
5:00 Introduction (J. W. Adelberg)
5:05 P-20 Design
of Multivariate Experiments
William C.
Bridges, Clemson University
5:20 P-21 Optimizing
Macro-nutrients for Stage II and Stage III of Micropropagation
Using Response Surface Methodology
Jeffrey W. Adelberg, Clemson University
5:35 P-22 Visualization,
Interpretation, and Mining of Data from Multivariate Designs
Randall
P. Niedz, USDA/ARS
5:50 Discussion
MARINE INVERTEBRATE
AND FISH CELL LINE ESTABLISHMENT
Convener: James J. Grasela,
USDA/ARS/BCIRL
5:00
pm – 6:00 pm Animal
Roundtable Meeting
Room 3
This
roundtable discussion aims to provide information on recent developments in
marine invertebrate and fish cell culture and their potential uses and
applications. This session will focus on
in vitro methodologies for developing aquatic animal cell cultures in general,
and specific requirements for marine invertebrate and fish tissue culture.
Other topics to be discussed will include the mechanistic approaches for
immortalizing aquatic invertebrate cells, and the
potential uses of marine invertebrate and fish cell lines in aquatic animal
health, biotechnology, and environmental monitoring.
Panelists:
David
Barnes, Mount Desert Island Biological
Laboratory
Lucy
E. J. Lee,
Wilfrid Laurier University
Shirley A. Pomponi,
Harbor Branch Oceanographic Institution
Guy
Smagghe, Ghent University
PLANT BIOTECHNOLOGY
CONTRIBUTED PAPER SESSION
Moderator: John J. Finer, OARDC/The Ohio State University
5:00
pm – 6:00 pm Plant
Contributed Paper Session Meeting
Rooms 8&9
5:00 P-1012 Green
Genetic Engineering Technology: Rearrangement of Endogenous Functional Genetic
Elements to Create Improved Grapvines
Dennis
J. Gray, University of Florida-IFAS, Z. T. Li, S. A. Dhekney, D. L. Hopkins, and T. W. Zimmerman
5:15 P-1013 Facilitation
of GFP Visualization in Green Tissues Using Bleaching Herbicides
John J. Finer, OARDC/The Ohio State University
5:30 P-1014 Over
Expression of AtNHX1 Gene in Transgenic Salt Tolerant Cultivated Tomato
Nanna Rama Swamy, Kakatiya University, Praveen Mamidala,
and Hongxia Zhang
5:45 P-1015 Qualitative
Analysis of Aloe Vera: Commercially Important Medicinal Plant Through HPLC and Clonal
Propagation of ChemoProfiled Material Through In
Vitro Techniques
Shailendra K. Tiwari, State Forest Research Institute, P. K. Shukla, Amit Pandey,
S. Mishra, M. P. Goswami,
and P. Bhargava
