TUESDAY, JUNE 17
LARGE
SCALE, CELL AND TISSUE-BASED PRODUCTION OF BIOPHARMACEUTICALS
AND SECONDARY METABOLITES
Conveners: Haruhiko Tsumura, Kirin Pharma Co.,
and Pamela J. Weathers,
Arkansas Bioscience Institute
8:00 am – 10:00 am Plenary
Symposium Presidio
I and II
This
session will cover relevant aspects of production of biopharmaceutical and
secondary metabolites by animal cells and plant cells and tissues in large scale.
The development of production cell lines, culture medium, metabolic
engineering, the use of genomic tools, bioreactor design and engineering, and
some case studies of actual commercial production will be
addressed. Where appropriate, speakers will address issues of economics.
Attendees will benefit by learning about progress made in novel approaches to
making bioproducts less expensive through innovation
and how the correct decisions in large scale culture
can improve downstream processing as illustrated by some success stories, along
with how to avoid some of the pitfalls.
8:00 Introduction
(H. Tsumura and P. J. Weathers)
8:05 PS-7 Animal and Plant Cultures: Production of
Biopharmaceuticals and Secondary Metabolites
Wayne Curtis, Pennsylvania State University
8:35 PS-8 Process Development for mAb
Therapeutic Production in 10,000 L-reactors with CHO
Cells
Chikashi Hirashima,
Chugai Pharmaceutical
9:00 PS-9 Air Lift Balloon Type Bioreactor: Platform
for Commercial Production of Plant Based Small Molecules and Tissues
Ganapathy Sivakumar, Arkansas State University
9:20 PS-10 Novel Plant Reactors for Pharmaceuticals
Production
Chunzhao Liu, Chinese Academy of Sciences
10:00 am – 10:30 am Coffee
Break Turquoise
Ballroom
HIGHLIGHT
OF PLANT GENOMICS RESEARCH AT THE UNIVERSITY OF ARIZONA
Convener: Wayne Parrott, University of Georgia
10:30 am – 12:30 pm Plant
Symposium Presidio
III and IV
The
term genomics was adopted in the late 1980’s to
encompass all aspects of mapping, sequencing and analysis of information of an
organism’s entire genome. Today, the field also includes the characterization
of genes, mRNAs and protein products (functional genomics), the study of DNA
and histone modification patterns (epigenomics) and the elucidation of evolutionary
relationships between genomes of different species (comparative genomics).
Scientists at the University of Arizona had greatly contributed to
the advancement of all aspects of plant genomics research. This session will
highlight pioneer work unraveling the molecular basis of paramutation,
the use of RNAi as a functional genomics tool and the
study of evolution in plants using comparative genomics.
10:30 Introduction
(L. B. Jacobsen and E. J. Roemer)
10:35 P-23 Interchromosomal
Transfer of Epigenetic Information
Vicki L. Chandler, University of Arizona
11:10 P-24 The iPlant Collaborative: A Cyberinfrastructure-Centered
Community for a New Plant Biology
Richard A. Jorgensen, University of Arizona.
11:45 P-25 The Oryza Map Alignment Project: Genomes in Flux
Rod A. Wing, Arizona Genomics Institute
12:20 Discussion
STEM
CELLS, PLASTICITY, AND REGENERATIVE MEDICINE
Conveners: Paul J. Price, D-Finitive Cell
Technologies, and Raziel S. Hakim, Howard University
10:30 am – 12:30 pm Animal
Symposium Coronado
I
Stem cells have long been recognized
as the source of mature differentiated cells in embryonic as well as mature
organ systems. While the terms totipotent, pleuripotent and multipotent have been used to describe stem cells by the
number of different mature cell types they can become, evidence is accumulating
that the culture environment in which cells are held can not only affect the
range of cell types that stem cells can become, but can even cause
reprogramming of mature cells. The term
transdifferentiation refers to mature cells which have been reprogrammed by environmental factors
to new fates. Current research indicates that stem and even mature cells of
post-natal origin can play a key role in cell-based therapies. The 3 speakers will
discuss their research results with different sources of post-natal stem cells.
10:30 Introduction
(P. J. Price and R. S. Hakim)
10:35 A-12 Mesenchymal
Stem Cells and the Development
of Therapeutics
Michelle Greene, Millipore Corporation
11:10 A-13 Mesenchymal Progenitors Able to Differentiate into Osteogenic, Chondrogenic, and/or Adipogenic Cells In Vitro are Present in Most Primary
Fibroblast-like Cell Populations
Kazuhiro Sudo, Riken BioResource Center
11:45 A-14 Induction of Hepatocyte-like Cells from Mesenchymal
Stem Cells and the Transplantation into Liver-injured Rats
Kiyohito Yagi, Osaka University
12:20 Discussion
Tuesday, June
17
Odd Poster
Authors will be present
1:30
pm – 2:30 pm
ANIMAL
CELL BIOTECHNOLOGY
Moderator: Kim O’Connor, Tulane University
1:30 pm – 3:30 pm Animal
Contributed Paper Session Coronado
I
1:30 A-1006 Multiendpoint
Mechanistic Profiling of Hepatotoxicants in HepG2/C3A Human Hepatoma Cells
and Comparison of Statistical Methods for Development of a Prediction Model for Acute Hepatotoxicity
Thomas
J. Flynn, US FDA, and Martine S. Ferguson
1:50 A-1007 In Vitro Cellular Response to Nanoparticle
Exposure
Julie
Elaine Morgan, Clayton State University, and J. A. Jordan
2:10 A-1008 Computational Tissue Engineering:
Monte-Carlo Simulation of Restructuring Dynamics During
Tissue Self-assembly of Prostate Cancer Spheroids
Kim
C. O’Connor, Tulane University, and H. Song
2:30 A-1009 Tools
for Genetic Characterization and Identification of Cell Lines
Manohar Rajeev Furtado,
Applied Biosystems, R. Fang, J. G. Shewale, and F. Hyland
2:50 A-1010 Mapping Signaling Pathways That
Control Gap Junction Function Using Modern Proteomic Approaches
Brad
Luther Upham, Michigan State University,
D. A. Whitten, C. G. Wiklerson, J. S. Park, I. Sovadinova, P. Babica, J. E. Trosko, and L. Blaha
3:10 A-1011 Online
Monitoring of Physiological Parameters of Cell Cultures
Ralf Ehret, Bionas
GmbH, Elke Thedinga, Sabine
Drechsler, Axel Kob, Marcus
Wego, Sebastian Rost,
Steffen Fürst, and Werner Baumann
STEM
CELL BIOLOGY AND CANCER DRUG DEVELOPMENT
Moderator:
Lia H.
Campbell, Cell and Tissue Systems, Inc.
2:30 pm – 3:30 pm Animal
Interactive Poster Session Turquoise
Ballroom
A-2008 Retaining
Cell Integrity During Organotypic
Model Viability Assays: Alternatives to MTT
Catherine
R. Kavanagh, State
University of New York-Stony Brook, L. J. Crawford, K. M. Sawicka, S. R. Simon, and E. J. Roemer
A-2009 In
Vitro Investigation of Antioxidant and Antiproteolytic
Properties of the Clove Extracts: Tellimagrandin
I and Casuarictin
Sumaira Zamurrad, State University of New York-Stony Brook, S. Parrino,
F. Daccueil, E. J. Roemer, and S. R. Simon
A-2010 Optimization of a New Method
of Characterizing
Live Basal Keratinocytes Using Chariot Transfection Reagent
Yusuke
Fukuda, State University of New York-Stony Brook,
E. J. Roemer, S. R. Simon, and M. Matic
A-2011 Comparison
of Growth Characteristics, Neurochemical
Parameters and Response to Toxicants for Neural Tissue Derived
Cell Lines from Goldfish and from Crayfish
Lucila E. J. Lee, Wilfrid Laurier University, M. R. Bufalino,
and M. P. Wilkie
A-2012 Assessing
the In Vitro Respiratory Toxicity of Fine Particles of Al2O3
and SiO2: a Precursor Study for Lunar
Dust Toxicity
Jacqueline A. Jordan, Clayton State University, A. M. Verhoff,
and D. G. Fischer
A-2013 D-Glucose
Protection Against MPP+
Induced Cell Death in Human Lung Carcinoma A549 Cell Line
David Fouad Elmashat, Florida A&M University, Ramesh
B. Badisa, and Karam F. Soliman
PLANT
TRANSFORMATION, RNAi, AND PHARMACEUTICAL/VALUE ADDED TRAITS
Moderator: Sandra L. Kelly, Agriculture and Agri-Food
Canada
2:30 pm – 3:30 pm Plant
Interactive Poster Session Turquoise
Ballroom
P-2006 Phloem
Specific Transgene Expression Driven by AtSUC2 Gene Promoter in Transgenic Citrus Plants to
Develop Citrus Greening Resistance
Ahmad
Al-Sayed Omar, University of Florida, Manjul Dutt, Gary Barthe, Vladimir Orbovic, and
Jude Grosser
P-2007 Genetic
Manipulation for Enhancing Calcium Uptake in Lettuce
Sung
Hun Park, Kansas State University, M. P Elless, J. Park, W. Lim, and K. D. Hirschi
P-2008 Optimization
of Transformation Efficiency in Flax
Sandra
L. Kelly,
Agriculture & Agri-Food Canada, and Mark C. Jordan
P-2009 Base-by-Base
Analysis of siRNA Production by a Plant Transgene
Victor Gaba,
ARO Volcani Center, Y. M. Shiboleth, S. Singer, E. Kukurt,
D. Liebmann, L. Maslenin,
A. Rosner, and A. Gal-On
P-2010 Wheat Virus Resistance Via
Interference RNA
Luisa F. Cruz,
Kansas
State
University,
John P. Fellers, and Harold N. Trick
P-2011 Partial Characterization and Purification of
Plant Derived Butyrylcholinesterase to Treat
Organophosphate Poisoning
Latha Kannan, Arizona State University B. C. Geyer, P.-E.
Garnaud, R. R. Woods, M. Muralidharan,
I Cherni, and T. S. Mor
IN
VITRO TOOLS, TECHNIQUES, AND OPTIMIZATION
Moderator: Michael E. Kane, University of Florida
3:30 pm – 5:00 pm Plant
Contributed Paper Session Presidio
I and II
3:30 P-1006 Seed Physiology of Bletia purpurea
(Pine Pink; Orchidaceae)—Fluctuating Low Temperature
and Dark Slow Development and Inhibit Germination
Timothy R. Johnson, University of Florida, M. E. Kane, and H. E. Perez
3:45 P-1007 Asymbiotic Seed Germination and In Vitro Seedling Development of Cyrtopodium punctatum: A
Propagation Protocol for an Endangered Florida Native Orchid
Daniela
Dutra,
University of Florida, M. E. Kane, and L. Richardson
4:00 P-1008 Synergistic Effect of Auxin and Cytokinin on In Vitro Androgenesis
in Azadirachta indica
A. Juss
Rakhi Chaturvedi,
Indian Institute of Technology-Guwahati, and Priyanka Srivastava
4:15 P-1009 Standardization of Protocol for
Efficient In
Vitro Clonal Propagation of Rare
Medicinal Plants e.g., Elaeocarpus & Capparis
L.
N. Shukla, B. R. Ambedkar
Bihar University, C. P. Shukla, B. K. Mishra, Manoj Kumar, Sushma Kumari, and T. Upadhyay
4:30 P-1010 Germplasm
Evaluation of Andographis paniculata (Kalmegh) Through Chemoprofiling for In Vitro Mass Multiplication of
Quality Germplasm from Satpura
Plateau Region of Madhya Pradesh
Shailendra Kumar Tiwari,
State Forest Research Institute, Vijay Bahadur, Amit Pandey, Shweta
Mishra, M. P. Goswani, and Pankaj Bhargava
4:15 P-1011 Micropropagation
of Boerhaavia diffusa
- A Valuable Medicinal Plant
Regha P. Periyannan, Muthyammal
College of Arts and Sciences, N. Vinod Kumar, M. S. Kavitha, M. Rajasekara Pandian, and E. G. Wesely
NEW
STRATEGIES FOR PRODUCTION OF SPECIALIZED METABOLITES
Convener: Fabricio
Medina Bolivar, ABI/Arkansas State University
3:30 pm – 5:00 pm Plant
Symposium Presidio
III and IV
Plants have evolved specialized networks for the biosynthesis
of a rich repertoire of natural products. These complex molecules serve as
chemical languages in ecosystems, and often confer protective characteristics
to plants allowing them to survive, and prosper in a multitude of challenging
ecological niches. Specialized metabolism is an economically important source
of fine chemicals, such as medicines, insecticides, dyes, flavors, and
fragrances. Scientists have made significant progress at developing strategies
to study and alter specialized metabolism. In particular, genomics and metabolomics approaches are leading to an advanced
understanding of how these metabolic networks function in a coordinated fashion
leading to desired traits or phenotypes. This symposium will provide an update
on novel strategies used by multidisciplinary teams to harness and alter
biosynthetic pathways for the production of natural products with diverse
bioactivities.
3:30 Introduction
(F. Medina-Bolivar)
3:35 P-26 Unraveling the Catalytic Specificity of Terpene Biosynthetic Enzymes and Engineering the
Biosynthesis of Novel Terpenes in Yeast and Plants
Joe Chappell, University of Kentucky
3:50 P-27 The Role of Ethnomedical Knowledge
in Defining Methods for Large-scale In Vitro Cultivation: Study Cases of Two
Mexican Medicinal Plants
María
Luisa Villarreal, Universidad Autónoma del Estado de
Morelos
4:25 P-28 Sub-lethal Levels of Electric Current
Elicit the Biosynthesis of Plant Secondary Metabolites
Evans Kaimoyo, University of Arizona
4:25 P-29 Engineering Ascorbate
for Enhanced Growth, Nutritional Content, and Stress Tolerance in Crops
Argelia Lorence, Arkansas State University
STEM
CELLS IN CANCER RESEARCH AND THERAPY
Conveners: Eugene Elmore, University of California – Irvine and Monika Schmelz,
University of Arizona Health Sciences Center
3:30 pm – 5:00 pm Animal
Symposium Coronado
I
One
hypothesis of cancer suggests that it arises from “cancer stem cells” that have
the ability, similar to all stem cells, to self-renew and to differentiate into
multiple cell types. Cancer stem cells
could persist in cancers and would be the likely cause of the tumor resistance
and relapse. The cancer stem cell theory
is one of many explanations have been offered for the resistance of various
cancers to therapy. Cancer stem cells
that survive therapy are potentially able to give rise to new cancers. Cells from cancer tissues are
generally characterized by their rapid growth rates compared to cells
from normal tissues, while stem cells tend to have much slower growth rates in vivo and would be more resistant to
therapies that depend upon cell division for efficacy. Cancer stem cells would therefore survive
therapy at doses that would kill most of the cells in a tumor, which would
explain the resistance and potential for relapse. Progress in stem cell biology has permitted
the isolation of stem cells from various embryonic and adult tissues, including
cancer tissues. If cancer stem cell-specific
biomarkers can be identified that are distinct from normal stem cell
biomarkers, research could then target the differences in normal and cancer
stem cell populations to identify specific therapies to target the cancer stem
cells. This would allow the development
of specific combination therapies to kill both the cancer and the cancer stem
cells. This could ultimately result in
better cancer survival rates and better quality of life for cancer patients.
The speakers in this session will address the cancer stem cell issue and provide
a critical update on the progress towards understanding the biology and future
applications.
3:30 Introduction
(E. Elmore and M. Schmelz)
3:35 A-15 Stem
Cells: Ancestors in a Somatic Cell Tree
Darryl K. Shibata, University of Southern California Keck School of Medicine
4:00 A-16 Mechanism of
Self-renewal of Brain Tumor Stem Cells
Ichiro Nakano, UCLA School of Medicine
4:25 A-17 Prostate
Tissue Homeostasis
Monika
Schmelz, University of Arizona Health Sciences Center
4:50 Discussion
5:00 pm –
5:30 pm SIVB
Business Meeting Presidio V
(All Members Are Urged to Attend)
Student Award Presentations
5:45 pm
– 10:00 pm An
Evening at Old Tucson Studios Old
Tucson Studios
Admittance
by Advance Ticket Holders Only