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2008 In Vitro Biology Meeting, Sunday, June 15

Sunday, June 15

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For your viewing convenience, the 2008 In Vitro Biology Meeting
Final Program has been broken down by day.

Abstracts & Posters

Keynote Symposium

Plenary Symposia

Animal Symposia
Plant Symposia

Invertebrate Conference Symposia

Education Symposia

Animal Contributed Papers
Plant Contributed Papers

Animal Posters
Plant Posters

Education Posters

2008 Abstract Index





7:00 am – 5:30 pm                                          Registration                    Presidio Registration Area





Conveners:        Amy A. Wang, GlaxoSmithKline, and David D. Songstad, Monsanto Company


8:00 am – 10:00 am                                   Plenary Symposium                             Presidio I and II


RNA-based technologies, namely, antisense oligonucleotides (ASOs), RNA interference (RNAi), and microRNA (miRNA), are a relatively new addition to the field of nucleic acid-based therapeutics.  ASOs have been tested in the clinic for ten years, and have one product on the market.  RNAi is a new preferred technique for specifically blocking gene expression so cellular phenotype and gene function can be studied.  The pioneers of RNAi research have been awarded Nobel Prize in 2006.  The revolutionary breakthrough of RNAi is having a major impact on drug discovery and development.  The newly emerging technology of miRNAs may have therapeutically useful links to cancer and other diseases.  The speakers in the plenary symposia will describe the new RNA-based technologies and their applications in both animal and plant research fields. They will provide up-to-date information on RNAi and miRNA products, and review recent progress in RNA-based drug discovery and development.


8:00                  Introduction (A. A. Wang and D. D. Songstad)

8:05      PS-1     Moving from In Vitro to In Vivo RNAi
Chris Cunning, Invitrogen Corporation

8:40      PS-2     New Development of microRNA Research and Role of miR-34s in p53 Tumor Suppressor Network
Caifu Chen, Applied Biosystems

9:15      PS-3      Control of Coleopteran Insect Pests Through RNA Interference

Jim Roberts, Monsanto Company

9:50                              Discussion




10:00 am  10:30 am                                     Coffee Break                             Turquoise Ballroom




Conveners:        Melissa E. Hinga, RiceTech, Inc., and David D. Songstad, Monsanto Company


10:30 am – 12:00 pm                                   Plant Symposium                            Presidio III and IV


The UN predicts that the world’s population will be approaching 8.5 billion by 2030 while the number of people living in rural areas will decrease to 40% of the world’s population (source: UN Dept Eco. and Soc. Affairs).  The increasing number of dwellers will put more pressure on fresh water supplies.  Higher crop yields will be required to feed the largely urban population.  Crops that are capable of using water more efficiently while maintaining or increasing grain or biomass yield must be developed.  Biotechnology provides one of the tools to assist in developing these water use efficient plants.  Identification of pathways responsible for increased stress tolerance will ultimately result in the transfer of novel genes for improved water use efficiency leading to improved crop performance.  In conjunction with the discovery of new traits via biotechnology, conventional breeding and molecular breeding provide additional tools to deliver these new traits in elite germplasm to provide a new generation of stress tolerant crops for farmers.  Today’s progress toward drought tolerant plants will lay the foundation for continuing to feed the world.


10:30                Introduction (M. E. Hinga and D. D. Songstad)

10:35    P-1       Improved Yield Stability of Maize Under Drought Stress

                        Michael H. Luethy, Monsanto Company

11:00    P-2       Genetic and Chemical Approaches to Delivering Abiotic Stress Tolerance in Crops

                        T. Lynne Reuber, Mendel Biotechnology

11:25    P-3       Transgenic Approaches Towards Improved Drought Tolerance in Turf and Forage Grass (Paspalum notatum Flugge)

                        Fredy Altpeter, University of Florida

11:50                Discussion




Conveners:        John W. Harbell, Mary Kay, Inc., and Guy Smagghe, University of Ghent


10:30 am – 12:30 pm                                 Animal Symposium                                    Coronado I


Toxicology has traditionally relied on the use of surrogate species to predict the impact of chemicals on the species of interest (e.g. man). Generally, the surrogate species (i.e., rodents and lagomorphs) were chosen for convenient manipulation in the laboratory rather than a demonstrated physiological match to the human. Most regulatory toxicology follows this pattern and it is the response in the surrogate species rather than the expected response in humans that drives regulatory decisions. Over the past few decades, there has been a strong interest in replacing the surrogate animal tests with physiologically relevant in vitro assays. This process has begun slowly but is now gaining momentum. There is now a general consensus on how in vitro methods should be validated and formal review processes are in place. A number of new methods have approved for regulatory use and some are incorporated into international guidelines. This symposium will focus on three areas: 1) validation, review and acceptance procedures for new in vitro methods; 2) post approval application and improvements of these methods; and 3) the future paradigm proposed by the National Academy of Sciences to shift from surrogate species to identification of toxic pathways using human cells.



10:30                Introduction (J. W. Harbell and G. Smagghe)

10:35    A-1       Japanese Collaboration on Alternative to Animal Toxicology Testing

                        Hajime Kojima, Japanese Center for the Validation of Alternative Methods

11:10    A-2       Critical Review of the Test Paradigm to Predict Human Hepatotoxicity

                        Phillippe Vanparys, Altoxicon

11:45    A-3       In Vitro Alternatives in Toxicology: A Current and Future Science Perspective

Eugene Elmore, University of CaliforniaIrvine




Conveners:        Michael E. Kane, University of Florida, and Scott L. Stewart, PhytoTechnology Laboratories, LLC


1:30 pm – 3:00 pm                                      Plant Symposium                            Presidio III and IV


In vitro ecology is a new concept in the study and use of in vitro techniques, which focuses on the use of in vitro technologies in the propagation, evaluation, improvement, and identification of unique plants and plant characteristics.  These characteristics can include ecotypic and genotypic differentiation, mass propagation and transgenic improvement of plants for habitat restoration, and the in vitro use of plants as tools for bioassays and ecological studies.  The study of in vitro ecology can lead to a better understanding the roles plant micropropagation, plant selection, and plant improvement play in our understanding of habitat restoration, plant mass propagation, habitat and plant ecology, and other in vitro technologies.  This session will define in vitro ecology by presenting topics current to the application of this research area, as well as help to define the potential future for in vitro ecological studies.  A short open discussion period will follow speaker presentations.


1:30                  Introduction (M. E. Kane and S. L. Stewart)

1:40      P-4       Stable Transformation of Freshwater Wetland Monocots and Its Ecological Implications

                        Suzanne M. D. Rogers, Fairmont State University

2:05      P-5       Using In Vitro Techniques as Tools to Differentiate Ecotypes of Calopogon tuberosus, a North American Native Orchid

                        Philip Kauth, University of Florida

2:25      P-6       Applications of Underwater Grass Cultures for Bioassays and Chemical Ecology Studies

                        Stephen Ailstock, Anne Arundel Community College

2:45                 Discussion




Conveners:        David Gidoni, The Volcani Center and Avraham A. Levy, The Weissman Institute of Sciences


1:30 pm – 3:00 pm                                      Plant Symposium                                Presido I and II


The precise integration of transgenes into plant genomes is critical for the controlled and stable expression of genes of interest.  It is necessary to avoid undesirable effects, such as gene silencing, mutation of host genes and position effects which may affect the specificity and stability of gene expression.  The ability to integrate DNA into a known site can also facilitate issues of GMOs regulation.  The integration of delivered DNA into a chromosomal sites can occur via three main pathways: 1- illegitimate, or non-homologous end joining, the most prominent pathway in plants;  2-  via gene targeting, the homologous recombination between the delivered DNA and a host chromosomal sequence; and 3- via recombinase-mediated site-specific recombination between a specific recognition site, present or introduced into the genome, and a delivered vector containing the compatible recognition sites.  This session will cover recent advances in all these fields.  For example, the engineering of nucleases (Zinc Finger Nucleases, or meganucleases) that can induce a DNA double strand break at any desired site, is an active field of research that will be addressed.  The induced break can then become a hotspot for DNA integration via homologous or non-homologous recombination with the delivered vector.  In addition, this session will emphasize novel sophisticated schemes of positive and negative selections that have been devised to select the targeted insertion of the delivered vector, via homologous or site-specific recombination. 


1:30                 Introduction (D. Gidoni and A. A. Levy)

1:35      P-7       Targeted Integration After Agrobacterium-mediated DNA Delivery

                        Paul J. Hooykaas, Leiden University

2:00      P-8       Towards Zinc Finger Nucleases-mediated Gene Targeting in Plants
Tzvi Tzfira, University of

2:20      P-9       Zinc Finger Nuclease-mediated Gene Targeting in Plants

                        Joseph F. Petolino, Dow AgroSciences

2:40      P-10     Plant Genome Manipulation Using Cre/lox Technology

                        Vibha Srivastava, University of Arkansas




Conveners:        Cynthia L. Goodman, USDA/ARS, and Guido F. Caputo, Natural Resources Canada


1:30 pm – 3:00 pm                                     Animal Symposium                                    Coronado I


The interactions between viruses and cells are quite complex and can be studied from many different angles.  How are viruses able to overcome the cells’ defenses and/or utilize host cell resources to proliferate?  What are some of the ways that cells attempt to combat viral invasion?  Insights from these questions can lead to improved treatments for human/livestock diseases or to novel means of controlling pest insects employing biocontrol agents.  Additionally, our understanding of virus-cell interactions can be used to develop innovative tools in areas such as gene transfer technology that are important for studying physiological aspects of biomedical and agricultural research.  Our session will focus on how viruses specifically interact with host systems and how these viral platforms can be used in the gene transfer arena.


1:30                  Introduction (C. L. Goodman and G. F. Caputo)

1:35      A-4       Baculovirus Genes Affecting Host Functions

                        Suzanne M. Thiem, Michigan State University

2:00      A-5       Coronavirus Assembly at Intracellular Membranes

                        Brenda G. Hogue, Arizona State University

2:25      A-6       Viral Gene Transfer Vectors in Studies of Human Smooth Muscle Function

                        William T. Gerthoffer, University of South Alabama

2:50                  Discussion







Society for In Vitro Biology (SIVB)

Japanese Association for Animal Cell Technology (JAACT)

The Japanese Tissue Culture Association (JTCA)


Program Chair:              David D. Ellis, USDA/ARS


3:00 pm – 5:30 pm                                     Opening Ceremony                              Presidio I and II


Conveners:        Todd J. Jones, BASF

                        David D. Ellis, USDA/ARS


3:00                  Welcome and Opening Remarks:

                        Todd J. Jones, President, Society for In Vitro Biology


3:05                  2008 Fellow Award Recipients (Awards to be presented at Section Meetings):

                        Michael E. Horn, Michael Horn & Associates LLC

                        Arthur H. McIntosh, USDA/AR/ BCIRL


3:15                  2008 Distinguished Service Award Presentations

                        (Awards to be presented by Paul J. Price):

David D. Ellis, USDA/ARS

David W. Altman, IPR Consulting, Inc.

Nancy A. Reichert, Mississippi State University

Richard Heller, University of South Florida

John W. Harbell, Mary Kay Inc.

Michael E. Horn, Michael Horn & Associates, LLC

Dennis A. Laska, Eli Lilly& Company


3:40                  2008 Lifetime Achievement Award Recipients:

                        (Introduction by Michael E. Horn; Acknowledgement by Dr. Widholm to follow)

                        Jack M. Widholm, University of Illinois


4:00                  Robert H. Lawrence, Jr. Keynote Symposium


                                    Introduction (T. J. Jones and D. D. Ellis)                                                              

KS-1     Global Agriculture at the Crossroads: Pathway to an Era of Biohappiness  

M. S. Swaminathan, Chairman of the M. S. Swaminathan Research Foundation, President of the Pugwash Conferences on Science and World Affairs; UNESCO Chair in Ecotechnology at the M. S. Swaminathan Research Foundation in Chennai, India; and Chairman of the National Commission on Agriculture, Food and Nutrition Security of India



5:30 pm – 6:30 pm             2008 WORLD CONGRESS ON IN VITRO BIOLOGY      Turquoise Ballroom

                                                    OPENING CEREMONY RECEPTION


5:30 pm – 7:00 pm                               Student Poster Competition                        Turquoise Ballroom



5:30 pm – 7:30 pm                                    Exhibits and Posters                             Turquoise Ballroom



Sunday, June 15

Odd Poster Authors will be present

6:30 pm – 7:30 pm





Conveners:        Nancy Jean Engelmann, University of Illinois-Urbana/Champaign, and Phillip J. Kauth, University of Florida



7:30 pm – 9:30 pm                                   Education Symposium                           Canyon Rooms



Bring your resume or CV and questions.  Our evening will consist of a brief formal program followed by interactive activities. We will begin with two presentations:

  1. Having the Right Stuff: when to prepare a CV vs Resume, what makes an outstanding CV or Resume, what supporting documents you should have with it.

  2. Interviewing Basics and the Differences between Academia vs. Industry: what you should be prepared to talk about and what skills may be expected of you.


We will then breakout for multiple interactions focused at several stations around the room. These will include:


  1. Opportunities for resume and CV review:  have the experts look at your CV or resume for critiquing and tips for improvement.  

2.       Mock interviewing: Practice answering the types of questions you may be asked at an interview within academia, industry or a government setting.  We will limit these to 10-15 min per person in order to allow time for multiple mock interviews per participant. Academic, industry and government representatives will be available so that you can explore the differences between the different environments.  Multiple mock interview sessions will also expose you to more than one interviewer’s style.  


7:30                  Introduction (N. J. Engelmann and P. J. Kauth)

7:35      E-5       Having the Right Stuff: Outstanding Resumes and CV’s for Outstanding Career Opportunities

                        Alaina G. Levine, University of Arizona

8:00      E-6       Interviewing Basics and the Differences between Academia vs. Industry

Gregory C. Phillips, Arkansas State University, and Jerry Ranch, Pioneer Hi-Bred International

8:25                  Workshop