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2008 In Vitro Biology Meeting, Monday, June 16

Monday, June 16

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For your viewing convenience, the 2008 In Vitro Biology Meeting
Final Program has been broken down by day.

Abstracts & Posters

Keynote Symposium

Plenary Symposia

Animal Symposia
Plant Symposia

Invertebrate Conference Symposia

Education Symposia

Animal Contributed Papers
Plant Contributed Papers

Animal Posters
Plant Posters


Education Posters

2008 Abstract Index

LATE SUBMISSION ABSTRACTS

MONDAY, JUNE 16

 

 

7:00 am – 6:00 pm                                          Registration                    Presidio Registration Area

 

 

BIOINFORMATICS, GENOMICS, PROTEOMICS AND CELLOMICS

Conveners:        Colette J. Rudd, XenoPort, Inc., and Mark C. Jordan, Agriculture and Agri-Food Canada

 

8:00 am – 10:00 am                                   Plenary Symposium                             Presidio I and II

                                                                             

New technologies with the potential to rapidly increase our understanding of the complexities within biological systems are becoming available. These include next generation sequencing technologies and advances in tools for rapid analysis of a broad profile of cellular constituents. As these technologies come into widespread use it will be essential for cell biologists to develop strategies to handle, analyze and integrate large quantities of data.

 

8:00                 Introduction (C. J. Rudd and M. C. Jordan)

8:05      PS-4    Next Generation Sequencing Technologies, Their Implications, and Prospects for Next-Next Gen Technologies

                        Jeffrey Schloss, National Human Genome Research Institute –National Institutes of Health

8:40      PS-5    Peptidomic Profiling of Endocrine Cell Culture Media for Bioactive Peptide Discovery

                        Steve Taylor, Amylin Pharmaceuticals

9:15      PS-6    Data Management and Extraction of Biological Information from Large Data Sets

                        David Mount, University of Arizona

9:50                  Discussion

 

 

10:00 am  10:30 am                                     Coffee Break                             Turquoise Ballroom

 

 

 

BIODIVERSITY FOR IMPROVING HUMAN HEALTH

Convener:         Argelia Lorence, ABI/Arkansas State University

 

10:30 am – 12:30 pm                                   Plant Symposium                               Presidio I and II

 

Nature is regarded as a main source of remedies throughout history.  Nowadays many cultures still rely on the large diversity, particularly of plants, as a source of medicines.  Importantly, more than 50% of small-molecule drugs introduced in the last years have been inspired or derived from a natural source. The advances in cell and tissue culture techniques applied to this rich biodiversity offer possibilities for the production and discovery of novel bioactive natural products with importance on human health.  In this session we will combine presentations that explore nature’s biodiversity, plant cell/tissue culture and novel in vivo biological assays for the production of natural products with applications as medicines and nutraceuticals.

 

10:30                Introduction (A. Lorence)

10:35    P-11     Searching for New Antiviral Agents from Brazilian Biodiversity

Claudia Maria Oliveira Simões, CCS Universidade Federal de Santa Catarina

11:00    P-12     Improving Cat’s Claw Alkaloid Production by Stimulating In Vitro Plant Cultures

Ana Ramos-Valdivia, CINVESTAV

11:25    P-13     Production and Neuroprotective Properties of Natural Resveratrol Analogs from Hairy Root Cultures of Peanut

Fabricio Medina-Bolivar, Arkansas State University

11:50    P-14     The Use of the Worm Caenorhabditis elegans as a Model to Investigate Functional Ingredients

Daniel Ramón Vidal, BIOpolis

12:15                Discussion

 

 

 

GLYCO-ENGINEERING OF PHARMA PROTEINS FROM PLANT CELLS

Conveners:        Michael E. Horn, Targeted Growth, Inc., and Vincent P. Wingate, Biolex

 

10:30 am – 12:30 pm                                   Plant Symposium                            Presidio III and IV

 

Most human therapeutic proteins are glycoproteins and this includes all of the monoclonal antibodies. Proper N-glycosylation is important for their proper folding of the protein, in vivo stability and biological activity in the patient. There are major similarities and important differences between the N-glycosylation pattern of plant and mammalian N-glycosylated proteins. The differences exist mainly in the late Golgi functions. For example, plants attach an a1,3 linked fucose residue to the middle of the chain while mammals attach an a1,6 fucose. Another example is that plants also attach a b1,2 xylose residue that is foreign in mammalian systems. These two differences have been implicated in immunogenicity studies. Some therapeutic proteins require a terminal sialic acid for complete functionality and plant cells do not generally synthesize this residue. The presentations in this session will cover a broad range of methods being successfully employed to humanize the N-glycosylation pattern of plant-made pharmaceutical proteins. These methods include the elimination or down-regulation of certain key endogenous enzymes as well as the addition of certain mammalian N-glycosylation enzymes.

 

10:30                Introduction (M. E. Horn and V. P. Wingate)

10:35    P-15     Humanization of N-glycosylation of Nicotiana benthamiana for Production of Biotherapeutics using MagnICON

Koen Weterings, Bayer BioScience NV

11:10    P-16     The Power of One: Glyco-optimized Therapeutic Antibodies in Lemna

                        John R. Gasdaska, Biolex

11:45    P-17     Sustainable Glyco-engineering and Production of Optimized Biopharmaceuticals in Bryophytes

                        Gilbert Gorr, Greenovation Biotech GmbH

12:20                Discussion

           

 

GOOD CELL CULTURE LABORATORY PRACTICES

Conveners:        John W. Harbell, Mary Kay, Inc., and Tetsuji Okamoto, Hiroshima University

 

10:30 am – 12:30 pm                                 Animal Symposium                                    Coronado I

 

Cell and tissue-based bioassays have been a mainstay for drug development, cancer research and basic cell physiology research for several decades. With the growth of molecular biology, genomics, and predictive in vitro toxicology, in vitro systems are being used by researchers of diverse backgrounds. Some researchers may see the cell cultures as simply another reagent. The study of cells in isolation provides many advantages and also considerable challenge. Often cited advantages include precise control over exposure conditions (concentration and duration), greater freedom in selection of the test species, use of well characterized cell types, and the ability to handle a large number of samples at a reasonable cost. The potential advantages can be realized only with a full understanding of the challenges. The initial characterization of the test system and maintenance of its homeostasis and consistency must be designed into the assay. The endpoints of the assay are necessarily indirect and often focus on the early cellular changes that precede the macroscopic changes in the whole organ or organism. This symposium will focus on assuring the validity of the test system as free from occult contamination and consisting of the cell type expected based on experience from several perspectives. In addition, it will address experimental design and the training required for proper execution of the studies.

 

10:30                Introduction (M. E. Horn and V. P. Wingate)

10:35    A-7       Mycoplasma Contamination and Cross Contamination in Tissue Culture: A Survey of Major Institutions in Japan

                        Arihiro Kohara, National Institute of Biomedical Innovation

11:10    A-8       Assuring Cell-based Assay Quality by Design and Execution: A Contract Research Laboratory Perspective

                        Hans A. Raabe, Institute for In Vitro Sciences

11:45    A-9       Homogenous Cell Cultures: Understanding Cross Contamination and Maintaining Culture Integrity

                        Amy Wright, Ciba Vision Corporation

12:20                Discussion

 

 

 

STUDENT NETWORKING LUNCHEON

Conveners:        Nancy Jean Engelmann, University of Illinois-Urbana/Champaign, and Phillip J. Kauth, University of Florida

 

12:30 pm – 2:00 pm                                 Education Symposium                                   Presidio V

 

Young scientists and their advisors are invited to attend this luncheon to discuss what they learned from Saturday and Sunday’s workshops.  This will also be an excellent opportunity for attendees to further network with the speakers and mock interviewers from Sunday night.  A final goal is to determine what other areas of career development are necessary to the young SIVB scientist and could be focused on for the 2009 Student Committee hosted session.

 

12:30                Introduction (N. E. Engelmann and P. J. Kauth)

12:40    E-7       Student Networking

                        David D. Songstad, Monsanto Company

 

 

 

Monday, June 16

Even Poster Authors will be present

1:30 pm – 2:30 pm

 

 

 

STEM CELL BIOLOGY AND CANCER DRUG DEVELOPMENT

Moderator:         Kim O’Connor, Tulane University

 

1:30 pm – 3:30 pm                          Animal Contributed Paper Session                          Coronado I

 

1:30         A-1000           Identification of a Homogeneous Adult Stem Cell Population, miR Signatures, and miR-Dependent Differentiation

                                    Frederick O. Cope, Ohio State University, and M. S. Blue

1:50         A-1001           Expansion of Pancreatic Stem Cells from Human Islet of Langerhans Preparations

                                    Lia H. Campbell, Cell and Tissue Systems, Inc., Nancy L. Parenteau, and Kelvin G. M. Brockbank

2:10         A-1002           Growth Factors and Extracellular Matrix Components Induce Formation of Membranes in Mouse Embryonic Stem Cells

                                    A. R. Calabro, St. John’s University, Frank A. Barile

2:30         A-1003          Melain Synthersis Inhibiting Activity of MC20 Isolated from a Marine Red Alga, Calliiarthron yessoense in B16 Melanoma Cells

                                   Yuto Kamei, Saga University, and Y. Ohtsuka

2:50         A-1004           Responses of Pineapple Stem Bromelain in a Human Cell Screening Assay for Melanoma Prevention

                                    Eugene Elmore, University of California-Irvine, Aarti Jain, Vernon E. Steele, and J. Leslie Redpath

3:10         A-1005           Anti-cancer Effect of Enzyme-digested Fucoidan Extract from Seaweed Mozuku

                                    Sanetaka Shirahata, Kyushu University, Kiichiro Teruya, Sakiko Matsuda, Ayumi Nakano, Takuya Nishimoto, Masashi Ueno, Kenji Shirouzu, Makiko Yamashita, Hiroshi Eto, and Yoshinori Katakura

 

 

IN VITRO ANIMAL CELL SCIENCES INTERACTIVE POSTERS

Moderator:         Lia H. Campbell, Cell and Tissue Systems, Inc.   

 

2:30 pm – 3:30 pm                           Animal Interactive Poster Session              Turquoise Ballroom

 

A-2000             Prostaglandin A2 Significantly Alters Gene Expression in an Established Insect Cell Line (BCIRL-HzAM1)

                        Cynthia L. Goodman, USDA/ARS/BCIRL, D. Stanley, Q. Song, S. An, and A. McIntosh

A-2001             Granulocytic Differentiation of HL-60 Promyelocytic Leukemia Cells is Associated with Increased mRNA Expression for Components of the Cullin-5 Containing E3 Ubiquitin Ligase

                        Shaneen S. Baxter, Midwestern University, Lauren A. Carlson, Alejandro M. S. Mayer, Mary L. Hall, and Michael J. Fay

A-2002             Organ and Monolayer Cell Culture of Gottingen Minipig Skin: A Model for Whole Skin Study and Drug Safety Screening

                        Michael K. Dame, University of Michigan Medical School, and Diana Spahlinger, Marissa DaSilva, Patricia Perone, Robert Dunstan, and James Varani

A-2003             Effects of Rheum ribes Ethylacetate Extracts on Cytochromes P450 1B1 Gene Expression and Glutathione-S-transferase Activity in HL-60 cells

                        Pembegul Uyar, Middle East Technical University

A-2004             In Vitro Investigation of Individual and Combined Cytotoxicity of Ochratoxin A and Arsenic in Chinese Hamster Lung Fibroblasts and Human Urothelial Cells

                        Manoj Aggarwal, Indian Veterinary Research Institute, C. Behm, W. Foellmann, J. K. Malik, and G. H. Degen

A-2005             In Vitro Evaluation of Nano-encapsulated Protective Antigen Functionality Through the Use of a Monomac 6 Assay  

Kevin E. Knockenhauer, State University of New York-Stony Brook, Katarzyna M. Sawicka, Elizabeth J. Roemer, and Sanford R. Simon

A-2006             Update on the COLIPA Eye Irritation Programme for Development of In Vitro Methods

                        Penny A. Jones, Unilever, Sandrine Bessou-Touya, Lieve Declercq, Ann De Smedt, Bart De Wever, Claudine Faller, John Harbell, Béatrice Le Varlet, Pauline McNamee, Monique Marrec-Fairley, Wolfgang Pape, Uwe Pfannenbecker, Klaus Schroeder, Magalie Tailhardat, Christine Van den Berghe, and Freddy Van Goethem

 

 

PLANT TISSUE CULTURE, MICROPROPAGATION AND SECONDARY METABOLITES 

Moderator:         Carol Potenza, USDA/ARS

 

2:30 pm – 3:30 pm                            Plant Interactive Poster Session               Turquoise Ballroom

 

P-2000              Secondary Metabolism of Hypericum perforatum Induced by Agrobacterium rhizogenes

Eliane R. Santarem, Pontificia Universidade Catolica do Rio Grande do Sul, D. C. Zamban, L. M. Felix, and L. V. Astarita

P-2001              Establishment and Rooting of In Vitro Micro-cuttings from Winter Buds of Quercus kelloggii

D. Kitterman, California State University, J. Johnson, and J. Bushoven

P-2002              Aseptic Coculture of Native Plant Derived Calli with Native and Nonnative Seedlings Affects Growth in a Dose Dependent and Contact Independent Manner

Carol Potenza, USDA/ARS, K. Yeater, D. James, and J. Barrow

P-2003             Iridoids Accumulation in Root Cultures of the Cancer Herb Castilleja tenuiflora        

Gabriela Trejo-Tapia, Centro de Desarrollo de Productos Bióticos-IPN, G. Rosas, A. Zamilpa, K. Bermudez, and M. Rodríguez

P-2004             Growth Patterns, Secreted Protein Profiles, and EST Transcripts from Sugar Beet (Beta vulgaris L.) Hairy-Root Cultures

Brett J. Savary, Arkansas State University, Prasanna Vasu, Daya Anandan, Ann C. Smigocki, and Alberto Nuñez

P-2005              In Vitro Studies of Tropical Woody Species

Somika Bhatnagar, Temasek LifeSciences Laboratory, S. Chandrasekharan, D.Y. Xie, and Y. Hong

 

 

ISSUES IN FIELD RELEASE OF TRANSGENIC PLANTS BY ACADEMIC INSTITUTIONS

Convener:          Tom Currier, Bayer CropScience

 

3:30 pm – 5:00 pm                                      Plant Symposium                            Presidio III and IV           

 

According to the “Recommendations for Management Practices for Field Trials with Bioengineered Plants” prepared by the National Agricultural Biotechnology Council*,  “application, institutional responsibility, approvals, training, fieldsite selection, record-keeping, communications, storage and disposal of biological materials, appropriate treatment of equipment including cleaning, monitoring, testing, and reporting are processes common to all research on transgenic plants”.  In order to test new transgenic plants in real conditions such as small-scale field studies, investigators at Universities and other institutions have to comply with federal and local regulatory requirements.  These regulations apply to all events not yet approved by the government agencies for unlimited commercial field release.  They require submission of information about the material to be planted, and extensive follow-up documentation of the field trials themselves.   A number of guidance documents have been developed by institutions to help investigators meet these needs.  Speakers at this session will describe their experiences in dealing with these requirements in the USA, and discuss how institutions can best develop their management practices to meet the requirements.  A round-table discussion of their experiences and those of other symposium participants will be held at the end of the session.

 

*http://nabc.cals.cornell.edu/pubs/Recomm_final.pdf

 

3:30                  Introduction (L. H. Campbell and L. B. Jacobsen)

3:35      P-18     Regulating Transgenic Plants for Academic Research

                        C. Neal Stewart, Jr., University of Tennessee

4:00      P-19     Field Evaluation of Regulated Transgenic Plants in an Academic                                                    Environment

                        Thomas E. Clemente, University of Nebraska-Lincoln

4:25      P-20     Controlled Field Release of Pharmaceutical Corn in Iowa: Lessons and Strategies

                        Kan Wang, Iowa State University

4:50                  Discussion

 

 

LEONARD J. SCHIFF MEMORIAL SYMPOSIUM:

CURRENT STATUS OF TISSUE-ENGINEERED PRODUCT REGULATION:

A GLOBAL VIEW ON THE RELATIONSHIP OF SCIENCE AND PRACTICALITY

Conveners:        Sandra L. Schneider, Research & Clinical Laboratory Systems, and Tohru Masui, JCRB Cell Bank

 

3:30 pm – 5:00 pm                                     Animal Symposium                                                Coronado I

 

Development of tissue-engineered cells, cellular products and therapeutic biologics requires an understanding of current regulatory issues.  Numerous mandate and guideline changes have occurred in United States (U.S.), European and Japanese law governing clinical studies.  As in vitro scientists, it is important to understand the key regulatory issues when conducting global pre-clinical and biologic research that have public interest and response.  The symposium objective is to build on previous Congress presentations related to regulatory pathways in discovery and development of biopharmaceuticals and cellular therapies.  This includes research design and other practical considerations associated with bench to bedside translational biologics.  The symposium outcome is to gain a global understanding of the essential US, European and Japanese regulatory process related to cellular products and transplantation of cultured cells.

 

3:30                  Introduction (S. L. Schneider and T. Masui)

3:35      A-10      The Regulation of Cultured Cells and Cellular Products for Transplantation: Current View of the Japanese Regulatory Process

                        Tohru Masui, JCRB Cell Bank

4:00      A-11      Navigating Research Strategy, Clinical Integrity and Current Global Regulatory Compliance

                        Sandra L. Schneider, Research & Clinical Laboratory Systems

 

4:10                  Roundtable/Panel Discussion

John W. Harbell, Mary Kay Inc.

Tohru Masui, JCRB Cell Bank

Quan Nguyen, Nguyen & Tarbet

Colette Rudd, Xenoport, Inc.

 

 

 

PLANT VACCINES, PHARMACEUTICALS, AND ALLERGEN REDUCTION

Moderator:         Mohammed Kamal Chowdhury, Claflin University

 

3:45 pm – 5:00 pm                           Plant Contributed Paper Session                                Presidio I and II

 

3:45      P-1001              Transformation of Tomato with Anti-malarial Genes with an Aim to Produce Edible Vaccines

                                    Mohammed Kamal Chowdhury, Claflin University, and Mihail Kantor

4:00      P-1002              In Planta Expression and Molecular Characterization of the Candidate HIV-1 Mucosal Vaccine CTB-MPR649-684

                                    Nobuyuki Matoba, Arizona State University, H. Kajiura, I. Cherni, J. D. Doran, M. Bomsel, K. Fujiyama, and T. S. Mor

4:15      P-1003              Production of Cervical Cancer-related HPV 16E7 as a Pharmaceutical Protein in Rice Seeds

                                    Amit Mehra, University of Arkansas, and Brad J. Murphy

4:30      P-1004              Plant-derived Intimin Vaccine to Prevent Colonization of Enterohaemorragic Escherichia coli

                                    Emel Topal, Biodesign Instittue at Arizona State University, Maria Lucrecia Alvarez, and Hugh S. Mason

4:45      P-1005              Elimination of the Three Major Allergens in Transgenic Peanut (Arachis hypogea L)

                                    Anthony Okello Ananga, Alabama A&M University, Hortense Dodo, and Koffi Konan

 

 

EFFECT OF MEDIUM CONSTITUENTS ON CELLS

Convener:           Paul J. Price, D-Finitive Cell Technologies

 

5:00 pm – 6:00 pm                                      Animal Workshop                                                  Coronado I

 

Eukaryotic cell culture media contain a diverse mixture of nutrient constituents, particularly formulations designed for serum-free culture.  Optimization of nutrient additives to cultivate specific cell types or to produce high yields of biological product requires consideration beyond intermediary metabolism of the independent nutrients.  This workshop explores interdependencies of nutrient constituents and external factors that impact stability or efficacy.

 

5:00      Introduction (P. J. Price)

5:05      Hydrolysates                                                                                 

            Matt Caple, SAFC Biosciences

5:10      Nutrient Feeding                                                                            

            Tom Fletcher, Irvine Scientific

5:15      Factorial Design Made Easy                                                           

            Steve Peppers, Invitrogen

5:20      Effect of Temperature and Light on Medium Constituents                   

            Paul Price, D-Finitive Cell Technologies

5:25      Panel discussion

5:55      Concluding Remarks

            Paul Price, D-Finitive Cell Technologies

 

 

EXPERIMENTAL DESIGN FOR IN VITRO BIOLOGISTS

Convener:           Randall P. Niedz, USDA /ARS

 

5:00 pm – 6:00 pm                                       Plant Workshop                                         Presidio III and IV

 

Workshop participants will discuss and learn some of the conceptual and practical aspects of how to plan and design multivariate experiments – as opposed to the mechanistic details of how to make certain types of statistical calculations.  Because experimental design is inherently geometric, the workshop will focus on how to think about the ‘geometry’ of experiments as defined by the independent factors, their ranges and the responses of interest to be measured.  The relative merits of multivariate versus one-factor-at-a-time (OFAT) approaches will be explored.  Primary discussion topics will include 1) how to conceptually design an experiment from a geometric perspective; 2) how to evaluate the “quality” of the resulting design; 3) how to evaluate the “quality” of measured data; and 4) how to select and utilize software applications to facilitate the conversion of the researcher’s subject matter expertise into statistically robust experimental designs.  Additionally, time will be allocated for discussion of specific research questions from participants

 

5:00      Introduction (R. P. Niedz)

5:05      Discussion

            Terrance J. Evens, USDA/ARS

            Randall P. Niedz, USDA/ARS

 

 

MICROSCOPY TOOLS FOR THE PLANT BIOTECHNOLOGIST

Convener:          Mary Welter, Dow AgroSciences

 

5:00 pm – 6:00 pm                                      Plant Symposium                                                  Presidio V

 

The most fundamental processes associated with plant cell biology and gene expression are sub-cellular in nature.  As such, microscopy plays a central role in enabling critical observations to be made.  Live cell imaging, FISH, FRET and laser capture microdissection are but a few of the microscopy-based techniques currently used to describe sub-cellular phenomenon in plants.  Although many of these techniques have become highly standardized and relatively simple to use, the risk of misinterpretation can be great if the methods used are not well understood and systematically implemented.  This workshop will explore some of the potential applications and inherent limitations of several of these methods.

 

 

5:00                  Introduction (M. Welter)

5:05      P-21     Imaging Live Plant Cells and Plant Cells

                        Sidney L. Shaw, Indiana University

5:25      P-22      Plant Microscopy: Perils and Premises

                        Elison B. Blancaflor, The Samuel Roberts Noble Foundation Inc.

5:45                  Discussion