MONDAY, JUNE 16
Conveners:
Colette J. Rudd, XenoPort,
Inc., and Mark C. Jordan, Agriculture
and Agri-Food Canada
8:00
am – 10:00 am Plenary
Symposium Presidio
I and II
New technologies
with the potential to rapidly increase our
understanding of the complexities within biological systems are becoming
available. These include next generation sequencing technologies and
advances in tools for rapid analysis of a broad profile
of cellular constituents. As these technologies come into widespread use
it will be essential for cell biologists to develop strategies to
handle, analyze and integrate large quantities of data.
8:00 Introduction (C. J. Rudd and M.
C. Jordan)
8:05 PS-4 Next
Generation Sequencing Technologies, Their Implications, and Prospects for
Next-Next Gen Technologies
Jeffrey Schloss,
National Human Genome Research
Institute –National
Institutes of Health
8:40 PS-5 Peptidomic Profiling of Endocrine Cell Culture Media for
Bioactive Peptide Discovery
Steve Taylor, Amylin Pharmaceuticals
9:15 PS-6 Data
Management and Extraction of Biological Information from Large Data Sets
David Mount, University of Arizona
9:50 Discussion
10:00 am – 10:30 am Coffee
Break Turquoise
Ballroom
BIODIVERSITY FOR IMPROVING
HUMAN HEALTH
Convener: Argelia Lorence, ABI/Arkansas State University
10:30 am – 12:30 pm Plant
Symposium Presidio
I and II
Nature is regarded
as a main source of remedies throughout history. Nowadays many cultures
still rely on the large diversity, particularly of plants, as a source of
medicines. Importantly, more than 50% of small-molecule drugs introduced
in the last years have been inspired or derived from a natural source. The
advances in cell and tissue culture techniques applied to this rich
biodiversity offer possibilities for the production and discovery of novel
bioactive natural products with importance on human health. In this session we will combine presentations that explore nature’s
biodiversity, plant cell/tissue culture and novel in vivo biological
assays for the production of natural products with applications as medicines
and nutraceuticals.
10:30 Introduction (A. Lorence)
10:35 P-11 Searching
for New Antiviral Agents from Brazilian Biodiversity
Claudia Maria Oliveira Simões, CCS Universidade Federal de Santa Catarina
11:00 P-12 Improving
Cat’s Claw Alkaloid Production by Stimulating In Vitro
Plant Cultures
Ana Ramos-Valdivia,
CINVESTAV
11:25 P-13 Production
and Neuroprotective Properties of Natural Resveratrol Analogs from Hairy Root Cultures of Peanut
Fabricio Medina-Bolivar, Arkansas State University
11:50 P-14 The Use of the
Worm Caenorhabditis elegans
as a Model to Investigate Functional Ingredients
Daniel Ramón Vidal, BIOpolis
12:15 Discussion
GLYCO-ENGINEERING OF PHARMA PROTEINS FROM PLANT CELLS
Conveners:
Michael E. Horn, Targeted Growth,
Inc., and Vincent P. Wingate, Biolex
10:30 am – 12:30 pm Plant Symposium Presidio III and IV
Most
human therapeutic proteins are glycoproteins and this
includes all of the monoclonal antibodies. Proper N-glycosylation
is important for their proper folding of the protein, in vivo stability
and biological activity in the patient. There are major similarities and
important differences between the N-glycosylation
pattern of plant and mammalian N-glycosylated
proteins. The differences exist mainly in the late Golgi
functions. For example, plants attach an a1,3 linked fucose residue to the
middle of the chain while mammals attach an a1,6 fucose.
Another example is that plants also attach a b1,2
xylose residue that is foreign in mammalian systems.
These two differences have been implicated in immunogenicity studies. Some therapeutic proteins require a
terminal sialic acid for complete functionality and
plant cells do not generally synthesize this residue. The presentations in this
session will cover a broad range of methods being
successfully employed to humanize the N-glycosylation
pattern of plant-made pharmaceutical proteins. These methods include the
elimination or down-regulation of certain key endogenous enzymes as well as the
addition of certain mammalian N-glycosylation
enzymes.
10:30 Introduction (M. E. Horn and V.
P. Wingate)
10:35 P-15 Humanization of N-glycosylation of Nicotiana benthamiana for Production of Biotherapeutics
using MagnICON
Koen Weterings, Bayer BioScience NV
11:10 P-16 The Power of One: Glyco-optimized
Therapeutic Antibodies in Lemna
John R. Gasdaska, Biolex
11:45 P-17 Sustainable
Glyco-engineering and Production of Optimized
Biopharmaceuticals in Bryophytes
Gilbert
Gorr, Greenovation Biotech GmbH
12:20 Discussion
GOOD CELL CULTURE LABORATORY
PRACTICES
Conveners: John W. Harbell,
Mary Kay, Inc., and Tetsuji Okamoto, Hiroshima University
10:30 am – 12:30 pm Animal
Symposium Coronado
I
Cell and
tissue-based bioassays have been a mainstay for drug
development, cancer research and basic cell physiology research for several
decades. With the growth of molecular biology, genomics, and
predictive in vitro toxicology, in vitro systems are being used by researchers
of diverse backgrounds. Some researchers may see the cell cultures as
simply another reagent. The study of cells in isolation provides many
advantages and also considerable challenge. Often
cited advantages include precise control over exposure conditions
(concentration and duration), greater freedom in selection of the test species,
use of well characterized cell types, and the ability
to handle a large number of samples at a reasonable cost. The potential
advantages can be realized only with a full
understanding of the challenges. The initial characterization of the test
system and maintenance of its homeostasis and consistency must
be designed into the assay. The endpoints of the assay are necessarily
indirect and often focus on the early cellular changes that precede the
macroscopic changes in the whole organ or organism. This symposium will focus
on assuring the validity of the test system as free from occult contamination
and consisting of the cell type expected based on experience from several
perspectives. In addition, it will address experimental design and the training
required for proper execution of the studies.
10:30 Introduction (M. E. Horn and V.
P. Wingate)
10:35 A-7 Mycoplasma Contamination and Cross Contamination in Tissue Culture: A
Survey of Major Institutions in Japan
Arihiro Kohara, National Institute
of Biomedical Innovation
11:10 A-8 Assuring
Cell-based Assay Quality by Design and Execution: A Contract Research
Laboratory Perspective
Hans A. Raabe, Institute for In Vitro Sciences
11:45 A-9 Homogenous
Cell Cultures: Understanding Cross Contamination and Maintaining Culture
Integrity
Amy Wright, Ciba Vision Corporation
12:20 Discussion
STUDENT NETWORKING
LUNCHEON
Conveners: Nancy
Jean Engelmann, University of Illinois-Urbana/Champaign,
and Phillip J. Kauth, University of Florida
12:30 pm – 2:00 pm Education
Symposium Presidio
V
Young scientists and their
advisors are invited to attend this luncheon to
discuss what they learned from Saturday and Sunday’s workshops. This will
also be an excellent opportunity for attendees to further network with the
speakers and mock interviewers from Sunday night. A final goal is to
determine what other areas of career development are necessary to the young
SIVB scientist and could be focused on for the 2009
Student Committee hosted session.
12:30 Introduction (N. E. Engelmann
and P. J. Kauth)
12:40 E-7 Student Networking
David D. Songstad, Monsanto Company
Monday, June 16
Even Poster Authors will be present
1:30 pm – 2:30 pm
STEM CELL BIOLOGY AND
CANCER DRUG DEVELOPMENT
Moderator: Kim
O’Connor, Tulane University
1:30 pm – 3:30 pm Animal Contributed
Paper Session Coronado
I
1:30 A-1000 Identification
of a Homogeneous Adult Stem Cell Population, miR
Signatures, and miR-Dependent Differentiation
Frederick
O. Cope, Ohio State University, and M. S. Blue
1:50 A-1001 Expansion of Pancreatic Stem Cells from Human Islet of
Langerhans Preparations
Lia H. Campbell, Cell and
Tissue Systems, Inc., Nancy L. Parenteau, and Kelvin G. M. Brockbank
2:10 A-1002 Growth Factors and Extracellular
Matrix Components Induce Formation of Membranes in Mouse Embryonic Stem Cells
A. R. Calabro, St. John’s University, Frank A. Barile
2:30 A-1003 Melain Synthersis Inhibiting Activity of MC20
Isolated from a Marine Red Alga, Calliiarthron
yessoense in B16 Melanoma Cells
Yuto Kamei, Saga University, and Y. Ohtsuka
2:50 A-1004 Responses of Pineapple Stem Bromelain
in a Human Cell Screening Assay for Melanoma Prevention
Eugene
Elmore, University of California-Irvine, Aarti
Jain, Vernon E. Steele, and J. Leslie Redpath
3:10 A-1005 Anti-cancer Effect of Enzyme-digested
Fucoidan Extract from Seaweed Mozuku
Sanetaka Shirahata, Kyushu University, Kiichiro Teruya,
Sakiko Matsuda, Ayumi
Nakano, Takuya Nishimoto, Masashi Ueno, Kenji
Shirouzu, Makiko Yamashita, Hiroshi Eto, and
Yoshinori Katakura
IN VITRO ANIMAL CELL
SCIENCES INTERACTIVE POSTERS
Moderator: Lia H. Campbell, Cell and Tissue
Systems, Inc.
2:30 pm – 3:30 pm Animal Interactive
Poster Session Turquoise
Ballroom
A-2000 Prostaglandin A2
Significantly Alters Gene Expression in an Established Insect Cell Line (BCIRL-HzAM1)
Cynthia L. Goodman, USDA/ARS/BCIRL, D. Stanley, Q. Song, S. An, and A. McIntosh
A-2001 Granulocytic Differentiation of
HL-60 Promyelocytic Leukemia Cells is Associated with
Increased mRNA Expression for Components of the Cullin-5
Containing E3 Ubiquitin Ligase
Shaneen S. Baxter, Midwestern University, Lauren A. Carlson, Alejandro M. S. Mayer, Mary L. Hall, and Michael J.
Fay
A-2002 Organ and Monolayer Cell Culture of
Gottingen
Minipig Skin: A Model for Whole Skin Study and
Drug Safety Screening
Michael K. Dame,
University of Michigan Medical School, and Diana Spahlinger,
Marissa DaSilva, Patricia Perone,
Robert
Dunstan, and James Varani
A-2003 Effects of Rheum ribes Ethylacetate Extracts
on Cytochromes P450 1B1 Gene Expression and
Glutathione-S-transferase Activity in HL-60 cells
Pembegul Uyar,
Middle East Technical University
A-2004 In Vitro Investigation of
Individual and Combined Cytotoxicity of Ochratoxin A and Arsenic in Chinese Hamster Lung
Fibroblasts and Human Urothelial Cells
Manoj Aggarwal, Indian Veterinary Research Institute, C. Behm, W. Foellmann, J. K. Malik, and G. H. Degen
A-2005 In Vitro Evaluation of Nano-encapsulated Protective Antigen Functionality Through the Use of a Monomac 6
Assay
Kevin
E. Knockenhauer, State University of
New York-Stony Brook,
Katarzyna M. Sawicka,
Elizabeth J. Roemer, and Sanford R. Simon
A-2006 Update on the COLIPA
Eye Irritation Programme for Development
of In Vitro Methods
Penny
A. Jones, Unilever,
Sandrine Bessou-Touya, Lieve Declercq, Ann De Smedt, Bart De Wever, Claudine Faller, John Harbell, Béatrice Le Varlet,
Pauline McNamee, Monique Marrec-Fairley, Wolfgang Pape, Uwe Pfannenbecker,
Klaus Schroeder, Magalie Tailhardat,
Christine Van den Berghe, and Freddy Van Goethem
PLANT TISSUE CULTURE,
MICROPROPAGATION AND SECONDARY METABOLITES
Moderator: Carol
Potenza, USDA/ARS
2:30 pm – 3:30 pm Plant Interactive
Poster Session Turquoise
Ballroom
P-2000 Secondary Metabolism of Hypericum perforatum Induced by Agrobacterium rhizogenes
Eliane R. Santarem, Pontificia Universidade
Catolica do Rio Grande do Sul,
D. C. Zamban, L. M. Felix, and L. V. Astarita
P-2001 Establishment and Rooting of In Vitro Micro-cuttings from Winter Buds of Quercus kelloggii
D. Kitterman, California State University, J. Johnson, and J. Bushoven
P-2002 Aseptic Coculture of Native
Plant Derived Calli with Native and Nonnative
Seedlings Affects Growth in a Dose Dependent and Contact Independent Manner
Carol Potenza, USDA/ARS, K. Yeater,
D. James, and J. Barrow
P-2003 Iridoids Accumulation in
Root Cultures of the Cancer Herb Castilleja tenuiflora
Gabriela Trejo-Tapia, Centro de Desarrollo de Productos
Bióticos-IPN, G. Rosas, A. Zamilpa,
K. Bermudez, and M. Rodríguez
P-2004 Growth Patterns, Secreted
Protein Profiles, and EST Transcripts from Sugar Beet (Beta vulgaris L.)
Hairy-Root Cultures
Brett J. Savary,
Arkansas State University, Prasanna Vasu, Daya Anandan,
Ann C. Smigocki, and Alberto Nuñez
P-2005 In Vitro Studies of Tropical Woody Species
Somika Bhatnagar,
Temasek LifeSciences
Laboratory, S. Chandrasekharan,
D.Y. Xie, and Y. Hong
ISSUES IN FIELD RELEASE
OF TRANSGENIC PLANTS BY ACADEMIC INSTITUTIONS
Convener: Tom
Currier, Bayer CropScience
3:30 pm – 5:00 pm Plant
Symposium Presidio
III and IV
According to the “Recommendations for Management
Practices for Field Trials with Bioengineered Plants” prepared by the
National Agricultural Biotechnology Council*, “application, institutional
responsibility, approvals, training, fieldsite
selection, record-keeping, communications, storage and disposal of biological
materials, appropriate treatment of equipment including cleaning, monitoring,
testing, and reporting are processes common to all research on transgenic
plants”. In order to test new transgenic
plants in real conditions such as small-scale field studies, investigators at
Universities and other institutions have to comply with federal and local
regulatory requirements. These regulations apply to all events not yet
approved by the government agencies for unlimited commercial field
release. They require submission of information about the material to be planted, and extensive follow-up documentation of the
field trials themselves. A number of guidance documents have been developed by institutions to help investigators
meet these needs. Speakers at this
session will describe their experiences in dealing with these requirements in
the USA,
and discuss how institutions can best develop their management practices to
meet the requirements. A round-table discussion of their experiences and
those of other symposium participants will be held at
the end of the session.
*http://nabc.cals.cornell.edu/pubs/Recomm_final.pdf
3:30 Introduction
(L. H. Campbell and L. B. Jacobsen)
3:35 P-18 Regulating
Transgenic Plants for Academic Research
C.
Neal Stewart, Jr., University of Tennessee
4:00 P-19 Field Evaluation of
Regulated Transgenic Plants in an Academic Environment
Thomas
E. Clemente, University of Nebraska-Lincoln
4:25 P-20 Controlled Field
Release of Pharmaceutical Corn in Iowa: Lessons and
Strategies
Kan Wang, Iowa State University
4:50 Discussion
LEONARD J. SCHIFF MEMORIAL
SYMPOSIUM:
CURRENT STATUS OF TISSUE-ENGINEERED PRODUCT REGULATION:
A GLOBAL VIEW ON THE RELATIONSHIP OF
SCIENCE AND PRACTICALITY
Conveners:
Sandra L. Schneider, Research &
Clinical Laboratory Systems, and Tohru Masui, JCRB Cell Bank
3:30 pm – 5:00 pm Animal
Symposium Coronado
I
Development of
tissue-engineered cells, cellular products and therapeutic biologics requires
an understanding of current regulatory issues.
Numerous mandate and guideline changes have occurred in United States
(U.S.), European and Japanese law governing clinical studies. As in vitro scientists,
it is important to understand the key regulatory
issues when conducting global pre-clinical and biologic research that have
public interest and response. The
symposium objective is to build on previous Congress presentations related to
regulatory pathways in discovery and development of biopharmaceuticals and
cellular therapies. This includes
research design and other practical considerations associated with bench to
bedside translational biologics. The
symposium outcome is to gain a global understanding of the essential US,
European and Japanese regulatory process related to cellular products and
transplantation of cultured cells.
3:30 Introduction
(S. L. Schneider and T. Masui)
3:35 A-10 The
Regulation of Cultured Cells and Cellular Products for Transplantation: Current
View of the Japanese Regulatory Process
Tohru
Masui, JCRB Cell
Bank
4:00 A-11 Navigating Research Strategy, Clinical
Integrity and Current Global Regulatory Compliance
Sandra
L. Schneider,
Research & Clinical Laboratory Systems
4:10 Roundtable/Panel
Discussion
John W. Harbell, Mary Kay Inc.
Tohru Masui, JCRB Cell
Bank
Quan Nguyen, Nguyen & Tarbet
Colette Rudd, Xenoport,
Inc.
PLANT
VACCINES, PHARMACEUTICALS, AND ALLERGEN REDUCTION
Moderator: Mohammed Kamal
Chowdhury, Claflin
University
3:45 pm – 5:00 pm Plant Contributed
Paper Session Presidio
I and II
3:45 P-1001 Transformation of Tomato with Anti-malarial Genes with an
Aim to Produce Edible Vaccines
Mohammed
Kamal Chowdhury,
Claflin University, and Mihail Kantor
4:00 P-1002 In Planta Expression
and Molecular Characterization of the Candidate HIV-1 Mucosal Vaccine CTB-MPR649-684
Nobuyuki
Matoba, Arizona State University, H. Kajiura, I. Cherni, J. D. Doran,
M. Bomsel, K. Fujiyama, and
T. S. Mor
4:15 P-1003 Production
of Cervical Cancer-related HPV 16E7 as a Pharmaceutical Protein in
Rice Seeds
Amit Mehra,
University of Arkansas, and Brad J. Murphy
4:30 P-1004 Plant-derived Intimin
Vaccine to Prevent Colonization of Enterohaemorragic
Escherichia coli
Emel Topal,
Biodesign Instittue at Arizona State University, Maria Lucrecia Alvarez, and Hugh S. Mason
4:45 P-1005 Elimination
of the Three Major Allergens in Transgenic Peanut (Arachis hypogea L)
Anthony Okello Ananga, Alabama A&M University, Hortense Dodo, and Koffi Konan
EFFECT OF MEDIUM CONSTITUENTS
ON CELLS
Convener:
Paul J. Price, D-Finitive Cell Technologies
5:00 pm – 6:00 pm Animal
Workshop Coronado
I
Eukaryotic cell culture
media contain a diverse mixture of nutrient constituents, particularly
formulations designed for serum-free culture.
Optimization of nutrient additives to cultivate specific cell types or
to produce high yields of biological product requires consideration beyond
intermediary metabolism of the independent nutrients. This workshop explores interdependencies of
nutrient constituents and external factors that impact
stability or efficacy.
5:00 Introduction (P. J. Price)
5:05 Hydrolysates
Matt Caple,
SAFC Biosciences
5:10 Nutrient Feeding
Tom Fletcher, Irvine Scientific
5:15 Factorial Design Made Easy
Steve Peppers, Invitrogen
5:20 Effect of Temperature and Light on Medium
Constituents
Paul Price, D-Finitive Cell Technologies
5:25 Panel discussion
5:55 Concluding Remarks
Paul Price, D-Finitive Cell Technologies
EXPERIMENTAL DESIGN
FOR IN VITRO BIOLOGISTS
Convener:
Randall P. Niedz,
USDA /ARS
5:00 pm – 6:00 pm Plant
Workshop Presidio
III and IV
Workshop
participants will discuss and learn some of the conceptual and practical
aspects of how to plan and design multivariate experiments – as opposed to the
mechanistic details of how to make certain types of statistical
calculations. Because experimental design is inherently geometric, the
workshop will focus on how to think about the ‘geometry’ of experiments as defined
by the independent factors, their ranges and the responses of interest to be measured. The relative merits of multivariate
versus one-factor-at-a-time (OFAT) approaches will be explored. Primary discussion topics will
include 1) how to conceptually design an experiment
from a geometric perspective; 2) how to evaluate the “quality” of the resulting
design; 3) how to evaluate the “quality” of measured data; and 4) how to select
and utilize software applications to facilitate the conversion of the researcher’s
subject matter expertise into statistically robust experimental designs.
Additionally, time will be allocated for discussion of
specific research questions from participants
5:00 Introduction (R. P. Niedz)
5:05 Discussion
Terrance J. Evens,
USDA/ARS
Randall
P. Niedz, USDA/ARS
MICROSCOPY TOOLS FOR THE PLANT BIOTECHNOLOGIST
Convener: Mary Welter, Dow AgroSciences
5:00 pm – 6:00 pm Plant
Symposium Presidio
V
The
most fundamental processes associated with plant cell biology and gene
expression are sub-cellular in nature.
As such, microscopy plays a central role in enabling critical
observations to be made. Live cell imaging, FISH, FRET and laser
capture microdissection are but a few of the microscopy-based techniques currently
used to describe sub-cellular phenomenon in plants. Although many of these techniques have become
highly standardized and relatively simple to use, the risk of misinterpretation
can be great if the methods used are not well understood and
systematically implemented. This
workshop will explore some of the potential applications and inherent
limitations of several of these methods.
5:00 Introduction (M. Welter)
5:05 P-21 Imaging Live Plant Cells
and Plant Cells
Sidney L. Shaw, Indiana University
5:25 P-22 Plant Microscopy: Perils and Premises
Elison
B. Blancaflor, The Samuel Roberts Noble Foundation Inc.
5:45 Discussion