Tuesday,
June 12
7:00 am 5:00 pm
Registration................... Grand Foyer
STRATEGIES TO CIRCUMVENT
LIMITATIONS FOR COMMERCIALIZATION IN ANIMAL AND PLANT SYSTEMS
Conveners: Wayne
Parrott, University of Georgia, and Todd J. Jones,
BASF Plant Sciences
8:00 am 10:00 am Plenary
Symposium................... Grand
3
Breakthroughs in technology whether in cloning
or in automated screening methods, have the ability to produce new traits and
products more rapidly, cheaply and economically in than ever before. This
session will highlight two cases studies from two companies one has made
several biological breakthroughs, and the other several technological
innovations, in essence developing a new paradigm that will play a greater role
in bringing the products of biotechnology to the market place.
8:00 Introduction (W. Parrot and T. J. Jones)
8:05 PS-7 From Cell Lines to Clones to Cows: Development & Production of
Recombinant Human Growth Hormone in Transgenic and Cloned Cattle
Carlos
Melo, Bio Sidus
9:00 PS-8 TraitMillTM: A High Throughput Functional
Genomics Platform for the Phenotypic Analysis of Cereals
Christophe Reuzeau, CropDesign NV
10:00 am 10:30 am Coffee
Break................... Grand 4
& 5
APPLICATION OF STEM CELL
TECHNOLOGY TO REGENERATIVE MEDICINE
Conveners: Linda
B. Jacobsen, Roche Applied Science and Elizabeth J. Roemer, State University of New York, Stony Brook
10:30 am 12:00 pm Animal
Symposium............ Senate 1
& 2
Stem cells are being studied in many fields of
biology, especially those focused on human health. Tumor stem cells have been
implicated in malignant cancers resistant to treatment. Stem cells also
offer great promise for the treatment of human disease. The focus of the
session will be on this potential for therapies that stem cells may provide for
diseases for which there is currently no satisfactory treatment. Our
first speaker will open with an overview of this developing therapeutic
field. The potential of the hematopoietic stem/progenitor cell population, isolated
from cord blood MNC, expanded in vitro for
therapeutic use will be described by our second speaker. The basic
biology of culturing stem cells and specific applications of stem cells in
cardiovascular disease will be discussed by our last speaker.
10:30 Introduction (L. B. Jacobsen and E. J. Roemer)
10:35 A-15 Stem Cells in
Regenerative Medicine
Debra
Grega, Case Western Reserve University
10:55 A-16 Commercializing
Hematopoietic Reconstitution Using Adult Cord Blood
Stem/Progenitor Cells Expanded in Culture: Challenges and Opportunities for
Industry
Paul
A. Hyslop, EndGenitor
Technologies, Inc.
11:15 A-17 Stem Cells in
Cardiovascular Disease
Keith
L. March, Indiana University
11:55 Discussion
FORAGE AND TURF BIOTECHNOLOGY
Conveners: Zeng-Yu Wang, The Samuel Noble Roberts Foundation, and Fredy Altpeter, University of Florida
10:30 am 12:30 pm Plant
Symposium..................... Grand
2
Forage grasses, forage legumes and turf grasses
are the backbone of sustainable agriculture and contribute extensively to the
world economy. They provide feed for
livestock or provide recreational spaces for sport and leisure while protecting
soil and water resources. Genetic improvement is one of the most effective ways
to increase productivity of forage and turf.
Due to the great complexity of forage and turf and the associated
difficulties encountered by traditional breeding methods, the potential of
molecular breeding for these species is evident. Advanced cellular and molecular technologies
are complementing conventional breeding in the development of improved germplasm. Speakers
in this session will highlight recent advances in forage and turf biotechnology
including transformation methods, biotic and abiotic
stress tolerance, forage and turf quality, use of
grass as biofuel crop, commercialization, risk
assessment and management.
10:30 Introduction (Z.-Y. Wang and F. Altpeter)
10:35 P-16 Genetic Engineering
of Stress Tolerance and Turf Quality in a Low-input Turfgrass
(Paspalum notatum
Flugge)
Fredy Altpeter, University of Florida
11:00 P-17 Transgenic
Approaches to Improve Quality and Abiotic Stress
Tolerance in Forage Crops
Zeng-Yu
Wang, The Samuel Roberts Noble Foundation
11:25 P-18 Field Evaluation of
Alfalfa Lines Down Regulated for Key Enzymes in the Lignin Biosynthetic Pathway
Stephen
J. Temple, Forage Genetics International
11:50 P-19 Transgenic Tall
Fescue for Fungal Disease Resistance and Transgenic Perennial Ryegrass for
Induced Self-elimination
Rongda Qu, North Carolina State University
12:20 Discussion
REGULATORY PERSPECTIVES:
COMPARING TRADITIONAL BREEDING VS. ENGINEERING - FROM DNA TO
THE MARKET
Convener: Dannette Ward, Monsanto Company/Regulatory, Wayne Parrott,
University of Georgia, and Raymond D. Shillito, Bayer CropScience
10:30 am 12:30 pm Plant Symposium..................... Grand
1
A decade has passed since the successful
commercialization of transgenic crops (often called GMO.s).
While conventionally modified crops via traditional breeding techniques
are essentially devoid of scrutiny, transgenic crops face a very complex
worldwide network of laws, regulations and treaties, which affect the ability
for farmers to plant and sell their crops, and shippers to move them
efficiently in the global market. We continue to see the
development of new and more stringent regulatory frameworks for transgenic
crops. Unfortunately, the cost of registering a crop in multiple
countries has resulted in the technology and thus the benefits only being
applied to major row crops. The hurdle of regulatory costs is
particularly difficult for academic institutions and small companies, who may
otherwise be able to provide great benefits from engineering a niche
crop. It has also undoubtedly delayed the introduction of beneficial
crops such as golden rice, and other crops, which may be able to reduce the
burden of hunger and malnutrition in the world. This session will
compare and contrast the process by which a conventional crop and a transgenic
crop is evaluated to achieve regulatory approval. Crops that may be marketed in one country
will be compared to those which must be registered in global markets to be
successful. This session will also examine the impact that
conventional crop breeding and domestication has on plant genomes, in an effort
to assess the extent to which the existing regulatory framework is
scientifically appropriate.
10:30 Introduction (D. Ward, W. Parrott, R. D. Shillito)
10:35 P-13 Regulatory
Perspectives: Comparing Traditional Breeding vs. Genetic Improvement
Susan
MacIntosh, MacIntosh
& Associates, Inc.
11:10 P-14 Comparative Case
Studies Differential Hurdles to the Same Trait Produced Conventionally vs. Transgenically
Jorge
Mayer, Campus Technologies Freiburg
11:45 P-15 Impact of
Conventional Modification the Plant Genome: How Regulations Ignore the Largest
Changes
Wayne
Parrott, University of Georgia
12:20 Discussion
Tuesday, June 12
Odd Poster Authors will be present
1:30 pm 2:30 pm
MICROPROPAGATION / ORGANOGENESIS / SECONDARY METABOLISM
Moderator: Patricia
Lynn Polowick, National Research Council Canada
2:30 pm 3:30 pm Plant Interactive Poster Session Grand
4 & 5
P-2007 Micropropagation of Juglans
nigra L. in Liquid Culture
Christian Roschke, Purdue University, and Paula M. Pijut
P-2008 Asymbiotic and Symbiotic Seed Germination of Eulophia alta
(Orchidaceae) Preliminary Evidence for Symbiotic
Culture Efficiency
Scott L. Stewart, University of Florida, T. R. Johnson, D. Dutra, M. E. Kane, and L. Richardson
P-2009 In
Vitro Production of Adaptogenic Phytoecdysteroids
from Ajuga turkestanica
Hairy Root Cultures
Diana
Meimei Cheng, University of Illinois, R. B. Rogers, M. A. Lila,
G. Yousef, and M. Grace
P-2010 High-tech
Production of Natural Anticancer Molecules from Plant Adventitious Roots Through Bioreactor Culture
Ganapathy Sivakumar, Arkansas Biosciences
Institute, and L. Bacchetta
P-2011 In Vitro Chemoprotective
Effect of Isolated culture of Nerium
oleander L.
Nelli Hovhannisyan, Yerevan State University
P-2012 Development and Analysis of Peas
with reduced Raffinose Oligosaccharide Content
Patricia Lynn Polowick, National Research
Council Canada, D. S. Baliski, C. A. Bock, and F. Georges
IN VITRO TOOLS AND EUKARYOTIC
CELL MODELS CELLULAR MODELS/IN VITRO TOOLS
Moderator: Guy
Smagghe, Ghent University
2:30 pm 3:30 pm Animal Interactive Poster Session Grand
4 & 5
A-2006 Achieve
New Levels of Expression of Transfected Tumor Cells
Linda
B. Jacobsen, Roche Applied Science, and Susan Calvin
A-2007 Rapid
Identification and Authentication of Animal Cell Culture Based on PCR Size Differences
Merry
Y. Liu, Center for Disease Control and Prevention, Hsi
Liu, Seh-Ching Lin, Angel Hughes, and Abbas Vafai
A-2008 Inflammation
of A549 Epithelial Cells An In Vitro Comparison of Various Mineral Oxides
Relative Cytotoxicities and their Impact Upon ROS Release
Philip
G. Claire, State University of New York-Stony Brook, A. K. Bushkanets, Y. Fukuda, S. R. Simon, and E. J. Roemer
A-2009 In
Vitro Evaluation of Reactive Oxygen Species Generation in Two Human Cell Lines
Exposed to Hurricane Katrina Flood Sediment Samples Containing Varying Pyrite
Content
Yusuke
Fukuda, State University of New York Stony Brook, P. G.
Claire, S. R. Simon, and E. J. Roemer
A-2010 Developing an In Vitro Assay to
Evaluate Human Cellular Responses to Reactive Mineral Oxides
Anna
Bushkanets, State University of New York Stony Brook, P. G.
Claire, Y. Fukuda, S. R. Simon, and E. J. Roemer
A-2011 The
Respiratory Syncytial Virus-F Protein Does Not
Activate the Neuroimmune System in Mice
Elise Marie Bendik, University of Illinois, D. R. Johnson, G. G.
Freund, and S. S. Korban
A-2012 Applications
of Neutral Red Uptake Assay for Basal Cytotoxicity
Assessment in Response to Viruses, Toxins, and Chemicals
Cheng
J. Cao, US Army Center for the Health Promotion and
Preventative Medicine, A. E. Chamber, M. M. Wade, and M. A. Major
A-2013 In Vitro Evaluation of
Antihypertensive Mechanism of the Dipeptide Val-Tyr in Rat Aorta Organ Bath Experiments
Guy Smagghe, Ghent University, L. Vercruysse, N. Morel, J. Szust,
and J. Van Camp
SECONDARY METABOLITES
Convener: Sylvia
A. Mitchell, University of the West Indies
3:30 pm 5:00 pm Plant Symposium..................... Grand
2
All plant cells produce primary
metabolites. Typically, primary metabolites
are found across all species within broad phylogenetic
groupings, and are produced using the same pathway (or nearly the same pathway)
in all these species. Secondary
metabolites, by contrast, are often species-specific (or found in only a narrow
phylogenetic group), and without these compounds the
organism suffers from only a minor impairment or no change in phenotype at
all. The function or importance of these
compounds to the organism is usually of an ecological nature as they are used
as defenses against predators, parasites and diseases, for interspecies
competition, and to facilitate the reproductive processes (coloring agents,
attractive smells, etc). Biomining is the process of seeking organisms for the
purpose of exploiting their natural products for drug or other technological
development. Obtaining secondary
metabolites in vitro, or biofarming, have been of
scientific and commercial interest for decades.
In this session, we will follow the history of secondary metabolite
production in vitro, point out the challenges and successes and point out some
interesting recent research that can help guide future endeavors. Secondary metabolites from tropical plants
will be highlighted while a case study of in vitro obtained secondary
metabolites of Catharanthus roseus
will also be presented.
3:30 Introduction
(S. A. Mitchell)
3:35 P-20 Overview of In
Vitro Secondary Metabolite Research - Challenges and Opportunities, Case Study
of Catharanthus roseus
Sylvia A. Mitchell,
University of the West Indies
3:55 P-21 Potential for In Vitro Manipulation and
Production of Tropical Secondary Metabolites
Sylvia
A. Mitchell, University of the West Indies
4:15 P-22 Controlled Environment Production: Key to
Consistency and Efficacy of Plant Medicinal Metabolites
Praveen K. Saxena,
University of Guelph
4:50 Discussion
THREE-DIMENSIONAL CONSTRUCTS
AND BIOMATERIALS FOR TISSUE ENGINEERING
Convener: Paul
J. Price, D-Finitive Technologies
3:30 pm 5:00 pm Animal Symposium.................... Grand
1
Our ability to develop phenotypically
normal 3-D human tissue constructs is moving rapidly. These proto-tissues will soon be playing key
roles in the areas of Regenerative Medicine, Cancer Biology and In Vitro
Toxicology testing. Key to the success
of this area of true Tissue Culture is using the right scaffold for the desired
results. Points to consider in the
choice of the scaffold include pore size, ease of modification, cell
interactions or microenvironment, mechanical strength, biodegradability and
absence of a foreign-body inflammatory reaction. This symposium will explore the strengths and
weaknesses of some of the various scaffold materials and successes in 3-D
culture.
3:30 A-18 Introduction to
Scaffolds for 3-D Cell Culture
Paul J. Price, D-Finitive Cell Technologies
3:50 A-19 3D Spheroid Cell
Culture Using Alginate Sponge Scaffolding
Richard M. Fike, Invitrogen
Corporation
4:25 A-20 Tissue
Engineering and 3-D Cell Culture Using Hyaluronan
Based Hydrogels
Anna Scott, Glycosan
BioSystems Inc.
5:00 pm 5:45 pm SIVB
Business Meeting............... Grand 3
(All Members are Urged to Attend)
6:00 pm 10:00 pm
An
Evening at the Indiana State Museum Event................ IN State Museum
Admittance
by Advance Ticket Holders Only