7:00 am – 5:30 pm Registration............................... Grand
Foyer
NEW DIRECTIONS IN VACCINE
DEVELOPMENT
Conveners: Delia
R. Bethell, Ventria Bioscience, and Michael E. Horn, Phyton Biotech
8:00 am – 10:00 am Plenary
Symposium................................ Grand 3
Vaccines have been hailed
as one of the most important public health achievements of the 20th century.
In the next five to 15 years, new vaccines and new vaccine delivery
technology will fundamentally change how clinicians prevent and treat disease,
with a substantial impact on public health. This symposium will explore some of
the new technologies currently being explored in vaccine development. Included
in the new technology is the development of DNA vaccines. This technology
targets the induction of a protective immune response by injecting DNA
sequences for the antigen of an infectious organism, which become incorporated
into the target cells, which then synthesize the protein antigen and result in
the host immune response. Another exciting area is the development of
vaccines by genetically engineering plants capable of expressing vaccine
antigens. Plants can be regenerated rapidly and provide the opportunity of cost
effective production of large scale vaccine antigens for purification or the
use of the plant itself in the immunization process through consumption of a
food for edible vaccines. Feeding the transgenic food material results in high
mucosal and serum antibody titers. Good lactogenic immunity has also been
shown in animals, which thus hold the promise of mother-to-child protection to
diseases such as HIV.
8:00 Introduction
(D. R. Bethell and M. E. Horn)
8:05 PS-1 Mechanisms
of Virus Neutralization by Antibodies as Determined Using Structural Approaches
Richard
J. Kuhn, Purdue University
8:40 PS-2 Plant-based
Vaccines
Stephen
Streatfield, Fraunhofer USA Inc
9:15 PS-3 Plant
Cell Culture Production of Recombinant Subunit Vaccines: Biochemical
Characterization and Regulatory Approval Process
Steven
Webb, Dow AgroSciences, LLC
9:50 Discussion
10:00 am – 10:30 am Coffee
Break............................... Grand 4 & 5
IN VITRO CELLULAR MODELS
IN DIABETES RESEARCH AND DEVELOPMENT
Convener:
Dennis A. Laska, Eli Lilly and Company
10:30 am – 12:00 pm Animal
Symposium................................. Grand 1
In vitro cellular models
are critical at all phases of pre-clinical evaluation of new drug candidates.
Pharmaceutical and biotechnology companies apply primary cell cultures and cell
lines in their discovery research efforts to uncover new targets, elucidate
disease mechanisms, and for selection of lead drug candidates for the treatment
of all diseases including type I and type II diabetes. This session will
highlight the application and innovation around in vitro models, as well as the
many challenges and hurdles faced in bringing a new drug to market for diabetes
treatment.
10:30 Introduction
(D. Laska)
10:35 A-1 Global
Diabetes Perspective and Diabetes R & D at Eli Lilly and Company
Anne
Reifel Miller, Eli Lilly and Company
10:52 A-2 Application
of Primary Islet Cultures in Diabetes Research
Martin
B. Brenner, Eli Lilly and Company - Germany
11:55 A-3 In
Vitro Models and Their Automation for Use in Screening Compounds and Selecting
Lead Therapeutic Candidates for Diabetes Treatment
Steven
Kahl, Eli Lilly and Company
11:38 A-4 The
Application of In Vitro Cell-based Models for Quality Assurance in the
Manufacture of Diabetes Therapeutics
Bhavin
S. Parekh, Eli Lilly and Company
BIO-BASED FEEDSTOCKS-BIOFUELS
Conveners: Nancy A. Reichert, Mississippi State University, and David D. Ellis, National Center for Genetic Resources
Preservation, USDA-ARS
10:30 am – 12:30 pm Plant
Symposium.................................. Grand 2
Plants have traditionally
been used to fulfill our food and feed needs, and increasingly our energy needs
as well. With fuel resources in limited and unequal supply globally, a much
greater emphasis is being placed on using plant-based biofeedstocks as
renewable energy resources. This symposium will discuss the state-of-the-art
in biofuels research. This will include the types of biofuels being generated
along with attributes and drawbacks, plus the identification and improvement of
plant species to fill the biofuels niche.
10:30 Introduction
(N. A. Reichert and D. D. Ellis)
10:35 P-1 The
Vision of Biofuels and Biorefining in the US: Opportunities for Biomass
Al
Darzins, National Bioenergy Center
11:10 P-2 Breaking
Barriers to Cellulose Ethanol: Role of Aqueous Pretreatments in Enzyme
Modifications of Plant Cell Walls
Michael
Ladisch, Purdue University
11:45 P-3 Camelina:
An Emerging Crop for Bioenergy
Duane
Johnson, Montana State University
12:20 Discussion
PLANT TRANSFORMATION: IMPROVED
VECTOR COMPONENTS
Moderators: Charles
Neal Stewart, Jr., University of Tennessee, and Liu Y. Shen, Dow Agrosciences
10:30 am – 12:30 pm Plant Contributed Paper Session...................... Grand
3
10:30 P-1000 Pigmented
Maize Seed Via Tissue-specific Expression of anthocyanin Pathway Gene
Transcription Factors
Liu
Y. Shen, Dow Agrosciences, and Joseph F. Petolino
10:45 P-1001 Evaluation
of Viral Suppressors of Silencing to Stabilize Transgene Expression
Taniya
Dhillon, OARDC/The Ohio State University, Joseph M. Chiera, and
John J. Finer
11:00 P-1002 The
Celery Mannose-6-phosphate Reductase Gene (M6PR): a Mannose-dependent, Bifunctional
Selectable Marker for Plant Transformation
Guo-qing
Song, Michigan State University, Wayne H. Loescher, and
Kenneth C. Sink
11:15 P-1003 Protoplast/GFP Transformation System:
Comparison Between Endoplasmic Reticulum Targeting and Non-targeting GFP in
Transgenic Citrus
Ahmad Al-Sayed Omar, University
of Florida, and J. W. Grosser
11:30 P-1004 Isolation
and Characterization of a Soybean (Glycine max) Polyubiquitin (Gmubi)
Promoter
John
J. Finer, OARDC/The Ohio State University, R. A. Bouchard, and J.
M. Chiera
11:45 P-1005 Excision
of a Selectable Marker Gene Mediated by Transactivated FLP Recombinase in
Tobacco Cells
David
Gidoni, The Volcani Center, B.-H. Ben Daniel, A. Mett, J. Feiler, I. Sobolev, N. Carmi, and
U. Nir
12:00 P-1006 A New
Red Fluorescent Protein from Coral is Effective for Transgenic Plant Studies
Charles Neal Stewart, Jr., University of Tennessee, Laura L. Good, Mary Rudis,
and Mikhail V. Matz
12:15 P-1007 Improvement
of Drought Tolerance in Canola Plants Expressing the Phosphotidylinositol
Specific Phospholipase C2 (PLC-2) Gene
Reda
Elwany Moghaieb, Cairo University, Sawsan S. Youssef, and Ahmed M. El-Sharkawy
ADVANCES IN PLANT BIOTECHNOLOGY
Moderators: Peggy
J. Ozias-Akins, University of Georgia, and Sawsan Samy Youssef, Cairo University
1:30 pm – 3:00 pm Plant Contributed Paper Session...................... Grand
3
1:30 P-1008 Development of a New Regeneration
and Transformation System for Impatiens
Yinghui
Dan, IALR/Virginia Tech, Aaron Baxter, and Richard E. Veilleux
1:45 P-1009 Application
of In Vitro Techniques for Micropropagation and Protection of Endemic and
Endangered Flowerbulbs of Turkey
Sebahattin
Özcan, Ankara University, İ. Parmaksız, S. Mirici, S. Çöçü, C. Sancak,
S. Uranbey, B. Gürbüz, A. İpek, D. Doğan-Kalyoncu, C. Karaoglu, C. S. Sevimay,
H.Ekiz, and N. Arslan
2:00 P-1010 Spontaneous
and Induced Variation in Peanut Seed Protein
Peggy
J. Ozias-Akins, University of Georgia, L. Ramos, P. Faustinelli, Y. Chu,
S. Maleki, J. Huntley, and J. Thelen
2:15 P-1011 Transformation
of Faba Bean (Vicia faba L.): a Non-tissue Culture Based Approach for
Generating Transgenic Plants
Sawsan
Samy Youssef, Cairo University, Reda E. A. Moghaieb, Mahmoud M. Saker,
Mohamed El Awady, and Ahmed El Sharkawy
2:30 P-1012 In
Vitro Multiplication and Cryopreservation of Hedychium Coronarium a Rare
Medicinal Plant of Central India
Shailendra
Kumar Tiwari, State Forest Research Institute, P. K. Shukla, Amit Pandey,
Ram Prakesh, and Pratibha Gour
2:45 P-1013 Zinc
Finger Nucleases, a New and Novel Method for Gene Targeting in Plants
Andriy
Tovkach, University of Michigan, Vardit Zeevi, and Tzvi Tzfira
CHARACTERIZATION OF EUKARYOTIC
CULTURES AND IDENTIFICATION &
CONTROL OF CONTAMINANTS
Convener: Yvonne
A. Reid, ATCC
1:30 pm – 3:00 pm Animal Symposium................................. Grand
2
New advances in transcriptomics,
proteomics and metabolomics research activities have lead to an increased use
of cell lines as research tools and models. However, as scientists
increasingly embrace a “systems biology” approach to research, problems
associated with cell line cross-contamination, misidentification and false
characterization will exponentially increase. Although acknowledged in
literature, the problem has persisted. Cellular cross-contamination and
variation in cell culturing conditions have contributed to erroneous,
misleading and inconsistent results. This session will focus on the impact of
cellular contamination and the lack of characterization of eukaryotic cultures
on research.
1:30 A-5 Why
It is Important to Authenticate and Characterize Your Cell Lines
Yvonne
A. Reid, ATCC
2:00 A-6 Development of an Optimized STR DNA
Marker System for Cell Identification and Contamination Detection
John Watson, Promega Corporation
2:30 A-7 Cell
Line Authentication by Isoenzyme Analysis: Lessons Learned in the Biotechnology
Industry
Raymond
Nims, Amgen BioReliance
MICROPROPAGATION &
THE ROLE OF GELLING AGENT
Conveners: Barbara
M. Reed, USDA, Valerie C. Pence, Cincinnati Zoo and Botanical Gardens, and
Michael J. Bosela, Indiana University-Purdue University at Fort Wayne
1:30 pm – 3:00 pm Plant Symposium.................................. Grand
1
The role of gelling agents
in the response of plants and cells in culture is often overlooked.
This symposium will explore the effects of the various types
of gelling agents on micropropagation and regeneration. Aside
from obvious effects on water availability and media hardness,
gelling agents can affect nutrient availability and in the case
of agar the non-gelling fractions of the product (agaropectin,
alginate, etc.) can influence plant morphogenesis. This session
will also provide basic information on gelling agent chemistry,
including an evaluation of their pH and salt requirements, and
will discuss issues of product variability both between manufacturers
and batches.
1:30
Introduction (B. M. Reed, V. C. Pence, and M. J. Bosela)
1:35 P-4 The
ABCs of Polysaccharide Gels
Rengaswami
Chandrasekaran, Purdue University - Whistler Center for Carbohydrate
Research
2:00 P-5 Gelling
Agent Modification for Large-scale Axillary Shoot Multiplication and Somatic
Embryogenesis of Pinus radiata for Commercial Forestry
Dale
Smith, MetaGenetics
2:25 P-6 A
Review of Gelling Agents Used for Plant Tissue Culture: Their Sources and
Characteristics
Ken
Torres, PhytoTechnology Laboratories
2:50 Discussion
2007 IN VITRO BIOLOGY OPENING
CEREMONY
Program Chair: John W. Harbell,
Mary Kay Inc.
3:00 pm – 5:30 pm Opening Ceremony.................... Grand
2
Conveners: Paul
J. Price, D-Finitive Cell Technologies
John
W. Harbell, Mary Kay Inc.
3:00 Welcome and
Opening Remarks:
Paul
J. Price, President, Society for In Vitro Biology
3:05 2007 Fellow Award
Recipients (Awards to be presented at Section Meetings):
Barbara
B. Doonan, UST Inc.
Todd
J. Jones, BASF Plant Sciences
J.
Denry Sato, Mount Desert Island Biological Laboratory
Guy
Smagghe, Ghent University
3:10 2007 Distinguished
Service Award Presentations:
David
Jayme, Brigham Young University – Hawaii
Wayne
Parrot, University of Georgia
3:20 2007 Lifetime Achievement
Award Recipients:
(Introduction
presented by Trevor A. Thorpe)
Indra
K. Vasil, University of Florida
Vilma
Vasil, University of Florida
3:50 SIVB Service
Recognition Presentation
(Introduction
presented by Nancy Reichert)
Robert H. Lawrence, Jr., UST Inc.
4:00 Robert H. Lawrence, Jr.
Keynote Symposium
Introduction
(P. J. Price and J. W. Harbell)
KS-1
NASA Space Based Research:
Challenges and Benefits for Tissue Engineering
David
A. Wolf, National Aeronautics and Space Administration
5:30 pm – 6:30 pm
2007 IN VITRO BIOLOGY MEETING
OPENING CEREMONY RECEPTION.................... Grand 4 & 5
5:30 pm – 7:30 pm Exhibits and Posters............. Grand
4 & 5
Sunday, June 10
Odd Poster Authors will be present
6:30 pm – 7:30 pm
STUDENT PROFESSIONAL DEVELOPMENT
– MENTORING
Conveners: Scott L. Stewart, University of Florida, and Randy Santiano,
Roche Diagnostics
7:30 pm – 9:00 pm Joint Symposium.................... Captiol
1
The role of mentoring in
undergraduate and graduate education is paramount to the success and
professional development of the student. Mentors provide sponsorship,
protection, challenge, exposure, visibility, counseling, acceptance,
confirmation, and coaching for students. Mentees can often provide challenge,
confirmation, and satisfaction for mentors. Mentors and the mentoring
relationship can have a large impact on students’ perceptions of the quality of
their undergraduate or graduate education. However, students often differ in
their conceptualizations of mentoring. The two most important things mentors
and mentees can do to make the mentoring relationship flourish are to
communicate clearly and effectively, and to provide honest feedback. This
symposium will provide various perspectives on the role and mechanics of
mentoring in undergraduate and graduate education, as well as in industry and
academic settings. These viewpoints will be elucidated through an
audience-lead panel discussion focused on the role of the mentor and mentee in
achieving educational outcomes.