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2007 In Vitro Biology Meeting, Sunday, June 10

Sunday, June 10

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For your viewing convenience, the 2007 In Vitro Biology Meeting
Final Program has been broken down by day.

Abstracts & Posters
Keynote Symposium
Plenary Symposia
Animal Symposia
Joint Symposium

Plant Symposia
Plant Contributed Papers
Animal Posters
Plant Posters

2007 Abstract Index

Addendum Abstracts

Sunday, June 10

7:00 am – 5:30 pm                     Registration............................... Grand Foyer




Conveners:             Delia R. Bethell, Ventria Bioscience, and Michael E. Horn, Phyton Biotech


8:00 am – 10:00 am             Plenary Symposium................................ Grand 3


Vaccines have been hailed as one of the most important public health achievements of the 20th century.  In the next five to 15 years, new vaccines and new vaccine delivery technology will fundamentally change how clinicians prevent and treat disease, with a substantial impact on public health.  This symposium will explore some of the new technologies currently being explored in vaccine development. Included in the new technology is the development of DNA vaccines.  This technology targets the induction of a protective immune response by injecting DNA sequences for the antigen of an infectious organism, which become incorporated into the target cells, which then synthesize the protein antigen and result in the host immune response.  Another exciting area is the development of vaccines by genetically engineering plants capable of expressing vaccine antigens. Plants can be regenerated rapidly and provide the opportunity of cost effective production of large scale vaccine antigens for purification or the use of the plant itself in the immunization process through consumption of a food for edible vaccines.  Feeding the transgenic food material results in high mucosal and serum antibody titers.  Good lactogenic immunity has also been shown in animals, which thus hold the promise of mother-to-child protection to diseases such as HIV.


8:00                         Introduction (D. R. Bethell and M. E. Horn)

8:05         PS-1        Mechanisms of Virus Neutralization by Antibodies as Determined Using Structural Approaches

                                Richard J. Kuhn, Purdue University

8:40         PS-2        Plant-based Vaccines

                                Stephen Streatfield, Fraunhofer USA Inc

9:15         PS-3        Plant Cell Culture Production of Recombinant Subunit Vaccines: Biochemical Characterization and Regulatory Approval Process

                                Steven Webb, Dow AgroSciences, LLC

9:50                         Discussion


10:00 am  – 10:30 am               Coffee Break............................... Grand 4 & 5




Convener:                              Dennis A. Laska, Eli Lilly and Company


10:30 am – 12:00 pm            Animal Symposium................................. Grand 1


In vitro cellular models are critical at all phases of pre-clinical evaluation of new drug candidates.  Pharmaceutical and biotechnology companies apply primary cell cultures and cell lines in their discovery research efforts to uncover new targets, elucidate disease mechanisms, and for selection of lead drug candidates for the treatment of all diseases including type I and type II diabetes.  This session will highlight the application and innovation around in vitro models, as well as the many challenges and hurdles faced in bringing a new drug to market for diabetes treatment.


10:30                       Introduction (D. Laska)

10:35       A-1          Global Diabetes Perspective and Diabetes R & D at Eli Lilly and Company

                                Anne Reifel Miller, Eli Lilly and Company

10:52       A-2          Application of Primary Islet Cultures in Diabetes Research

                                Martin B. Brenner, Eli Lilly and Company - Germany

11:55       A-3          In Vitro Models and Their Automation for Use in Screening Compounds and Selecting Lead Therapeutic Candidates for Diabetes Treatment

                                Steven Kahl, Eli Lilly and Company

11:38       A-4          The Application of In Vitro Cell-based Models for Quality Assurance in the Manufacture of Diabetes Therapeutics

                                Bhavin S. Parekh, Eli Lilly and Company




Conveners:             Nancy A. Reichert, Mississippi State University, and David D. Ellis, National Center for Genetic Resources Preservation, USDA-ARS


10:30 am – 12:30 pm             Plant Symposium.................................. Grand 2


Plants have traditionally been used to fulfill our food and feed needs, and increasingly our energy needs as well.  With fuel resources in limited and unequal supply globally, a much greater emphasis is being placed on using plant-based biofeedstocks as renewable energy resources.  This symposium will discuss the state-of-the-art in biofuels research.  This will include the types of biofuels being generated along with attributes and drawbacks, plus the identification and improvement of plant species to fill the biofuels niche.


10:30                       Introduction (N. A. Reichert and D. D. Ellis)

10:35       P-1           The Vision of Biofuels and Biorefining in the US: Opportunities for Biomass

                                Al Darzins, National Bioenergy Center

11:10       P-2           Breaking Barriers to Cellulose Ethanol: Role of Aqueous Pretreatments in Enzyme Modifications of Plant Cell Walls

                                Michael Ladisch, Purdue University

11:45       P-3           Camelina: An Emerging Crop for Bioenergy

                                Duane Johnson, Montana State University

12:20                       Discussion




Moderators:             Charles Neal Stewart, Jr., University of Tennessee, and Liu Y. Shen, Dow Agrosciences

10:30 am – 12:30 pm Plant Contributed Paper Session...................... Grand 3


10:30       P-1000     Pigmented Maize Seed Via Tissue-specific Expression of anthocyanin Pathway Gene Transcription Factors

                                Liu Y. Shen, Dow Agrosciences, and Joseph F. Petolino

10:45       P-1001     Evaluation of Viral Suppressors of Silencing to Stabilize Transgene Expression

                                Taniya Dhillon, OARDC/The Ohio State University, Joseph M. Chiera, and John J. Finer

11:00       P-1002     The Celery Mannose-6-phosphate Reductase Gene (M6PR): a Mannose-dependent, Bifunctional Selectable Marker for Plant Transformation

                                Guo-qing Song, Michigan State University, Wayne H. Loescher, and Kenneth C. Sink  

11:15       P-1003     Protoplast/GFP Transformation System: Comparison Between Endoplasmic Reticulum Targeting and Non-targeting GFP in Transgenic Citrus

                                Ahmad Al-Sayed Omar, University of Florida, and J. W. Grosser

11:30       P-1004     Isolation and Characterization of a Soybean (Glycine max) Polyubiquitin (Gmubi) Promoter

                                John J. Finer, OARDC/The Ohio State University, R. A. Bouchard, and J. M. Chiera

11:45       P-1005     Excision of a Selectable Marker Gene Mediated by Transactivated FLP Recombinase in Tobacco Cells

                                David Gidoni, The Volcani Center, B.-H. Ben Daniel, A. Mett, J. Feiler, I. Sobolev, N. Carmi, and U. Nir

12:00       P-1006     A New Red Fluorescent Protein from Coral is Effective for Transgenic Plant Studies

                                Charles Neal Stewart, Jr., University of Tennessee, Laura L. Good, Mary Rudis, and Mikhail V. Matz

12:15       P-1007     Improvement of Drought Tolerance in Canola Plants Expressing the Phosphotidylinositol Specific Phospholipase C2 (PLC-2) Gene

                                Reda Elwany Moghaieb, Cairo University, Sawsan S. Youssef, and Ahmed M. El-Sharkawy        




Moderators:             Peggy J. Ozias-Akins, University of Georgia, and Sawsan Samy Youssef, Cairo University


1:30 pm – 3:00 pm     Plant Contributed Paper Session...................... Grand 3


1:30         P-1008     Development of a New Regeneration and Transformation System for Impatiens

                                Yinghui Dan, IALR/Virginia Tech, Aaron Baxter, and Richard E. Veilleux

1:45         P-1009     Application of In Vitro Techniques for Micropropagation and Protection of Endemic and Endangered Flowerbulbs of Turkey

                                Sebahattin Özcan, Ankara University, İ. Parmaksız, S. Mirici, S. Çöçü, C. Sancak, S. Uranbey, B. Gürbüz, A. İpek, D. Doğan-Kalyoncu, C. Karaoglu, C. S. Sevimay, H.Ekiz, and N. Arslan

2:00         P-1010     Spontaneous and Induced Variation in Peanut Seed Protein

                                Peggy J. Ozias-Akins, University of Georgia, L. Ramos, P. Faustinelli, Y. Chu, S. Maleki, J. Huntley, and J. Thelen  

2:15         P-1011     Transformation of Faba Bean (Vicia faba L.): a Non-tissue Culture Based Approach for Generating Transgenic Plants

                                Sawsan Samy Youssef, Cairo University, Reda E. A. Moghaieb, Mahmoud M. Saker, Mohamed El Awady, and Ahmed El Sharkawy

2:30         P-1012     In Vitro Multiplication and Cryopreservation of Hedychium Coronarium a Rare Medicinal Plant of Central India

                                Shailendra Kumar Tiwari, State Forest Research Institute, P. K. Shukla, Amit Pandey, Ram Prakesh, and Pratibha Gour

2:45         P-1013     Zinc Finger Nucleases, a New and Novel Method for Gene Targeting in Plants

                                Andriy Tovkach, University of Michigan, Vardit Zeevi, and Tzvi Tzfira





Convener:                               Yvonne A. Reid, ATCC


1:30 pm – 3:00 pm                Animal Symposium................................. Grand 2


New advances in transcriptomics, proteomics and metabolomics research activities have lead to an increased use of cell lines as research tools and models.  However, as scientists increasingly embrace a “systems biology” approach to research, problems associated with cell line cross-contamination, misidentification and false characterization will exponentially increase.  Although acknowledged in literature, the problem has persisted.  Cellular cross-contamination and variation in cell culturing conditions have contributed to erroneous, misleading and inconsistent results.  This session will focus on the impact of cellular contamination and the lack of characterization of eukaryotic cultures on research.


1:30         A-5          Why It is Important to Authenticate and Characterize Your Cell Lines

                                Yvonne A. Reid, ATCC

2:00         A-6          Development of an Optimized STR DNA Marker System for Cell Identification and Contamination Detection

                                John Watson, Promega Corporation

2:30         A-7          Cell Line Authentication by Isoenzyme Analysis: Lessons Learned in the Biotechnology Industry

                                Raymond Nims, Amgen BioReliance




Conveners:             Barbara M. Reed, USDA, Valerie C. Pence, Cincinnati Zoo and Botanical Gardens, and Michael J. Bosela, Indiana University-Purdue University at Fort Wayne


1:30 pm – 3:00 pm                 Plant Symposium.................................. Grand 1


The role of gelling agents in the response of plants and cells in culture is often overlooked.  This symposium will explore the effects of the various types of gelling agents on micropropagation and regeneration.  Aside from obvious effects on water availability and media hardness, gelling agents can affect nutrient availability and in the case of agar the non-gelling fractions of the product (agaropectin, alginate, etc.) can influence plant morphogenesis.  This session will also provide basic information on gelling agent chemistry, including an evaluation of their pH and salt requirements, and will discuss issues of product variability both between manufacturers and batches.

1:30                         Introduction (B. M. Reed, V. C. Pence, and M. J. Bosela)

1:35         P-4           The ABCs of Polysaccharide Gels

                                Rengaswami Chandrasekaran, Purdue University - Whistler Center for Carbohydrate Research

2:00         P-5           Gelling Agent Modification for Large-scale Axillary Shoot Multiplication and Somatic Embryogenesis of Pinus radiata for Commercial Forestry

                                Dale Smith, MetaGenetics

2:25         P-6           A Review of Gelling Agents Used for Plant Tissue Culture: Their Sources and Characteristics

                                Ken Torres, PhytoTechnology Laboratories

2:50                         Discussion




Program Chair: John W. Harbell, Mary Kay Inc.


3:00 pm – 5:30 pm    Opening Ceremony.................... Grand 2


Conveners:        Paul J. Price, D-Finitive Cell Technologies

                        John W. Harbell, Mary Kay Inc.


3:00      Welcome and Opening Remarks:

            Paul J. Price, President, Society for In Vitro Biology


3:05      2007 Fellow Award Recipients (Awards to be presented at Section Meetings):

            Barbara B. Doonan, UST Inc.

            Todd J. Jones, BASF Plant Sciences

            J. Denry Sato, Mount Desert Island Biological Laboratory

            Guy Smagghe, Ghent University


3:10      2007 Distinguished Service Award Presentations:

            David Jayme, Brigham Young University – Hawaii

            Wayne Parrot, University of Georgia


3:20      2007 Lifetime Achievement Award Recipients:

            (Introduction presented by Trevor A. Thorpe)

            Indra K. Vasil, University of Florida

            Vilma Vasil, University of Florida


3:50      SIVB Service Recognition Presentation

            (Introduction presented by Nancy Reichert)

            Robert H. Lawrence, Jr., UST Inc.


4:00      Robert H. Lawrence, Jr. Keynote Symposium


            Introduction (P. J. Price and J. W. Harbell)          


KS-1     NASA Space Based Research: Challenges and Benefits for Tissue Engineering

            David A. Wolf, National Aeronautics and Space Administration


5:30 pm – 6:30 pm                 


OPENING CEREMONY RECEPTION.................... Grand 4 & 5


5:30 pm – 7:30 pm  Exhibits and Posters............. Grand 4 & 5


Sunday, June 10

Odd Poster Authors will be present

6:30 pm – 7:30 pm





Conveners:        Scott L. Stewart, University of Florida, and Randy Santiano, Roche Diagnostics


7:30 pm – 9:00 pm     Joint Symposium.................... Captiol 1


The role of mentoring in undergraduate and graduate education is paramount to the success and professional development of the student.  Mentors provide sponsorship, protection, challenge, exposure, visibility, counseling, acceptance, confirmation, and coaching for students.  Mentees can often provide challenge, confirmation, and satisfaction for mentors.  Mentors and the mentoring relationship can have a large impact on students’ perceptions of the quality of their undergraduate or graduate education.  However, students often differ in their conceptualizations of mentoring.  The two most important things mentors and mentees can do to make the mentoring relationship flourish are to communicate clearly and effectively, and to provide honest feedback.  This symposium will provide various perspectives on the role and mechanics of mentoring in undergraduate and graduate education, as well as in industry and academic settings.  These viewpoints will be elucidated through an audience-lead panel discussion focused on the role of the mentor and mentee in achieving educational outcomes.