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For your viewing convenience, the 2007 In Vitro Biology
Meeting
Final Program has been broken down by day.
- To view session descriptions, speakers, and titles for each day, please
click on Daily Program
- To review the day's schedule, click on Program Summary
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Monday, June 11
7:00
am 6:00 pm Registration................... Grand Foyer
IMMUNE SIGNAL TRANSDUCTION
ACROSS KINGDOMS
Conveners: Guy Smagghe, University of Ghent, and David
Stanley, USDA-ARS
8:00
am 10:00 am Plenary
Symposium................... Grand
3
Immunity is the
ability to protect the self from microbial invasion and infection.
Virtually all metazoan organisms express immune functions and these functions
are mediated and coordinated by various biochemical mechanisms. This
Plenary Session will present a broad view of the immune signaling mechanisms
that operate in plants, invertebrates and vertebrates. The overarching
goal of the program is an appreciation of the unity and diversity of immune
signal mechanisms in metazoans. The significance of the program lies in
its potential to link scientists into an interdisciplinary community of with a
common goal of understanding immunity.
8:00 Introduction (G. Smagghe
and D. Stanley)
8:05 PS-4 Plant Defense
Systems Mediated by Protein-carbohydrate Recognition
Els
Van Damme, Ghent University
8:40 PS-5 Insect Immunity
in Pseudoplusia includens:
Biowarfare Between Host,
Parasitoid, and Virus
Kevin D. Clark, University of Georgia
9:15 PS-6 TBD
Richard
Tapping, University of Illinois
9:50 Discussion
10:00 am
10:30 am Coffee Break................... Grand
4 & 5
BIOSENSORS
Convener: William J. Smith, US Army Medical
Research Institute of Chemical Defense, and Guy Smagghe,
University of Ghent
10:30 am 12:30 pm Animal Symposium............ Senate 1 & 2
Biosensors are
analytical devices incorporating a biological material associated with a
physicochemical transducer to produce a digital signal indicating the presence
of a specific analyte or related group of analytes. Biosensors
have found utility in medical, pharmaceutical, environmental and food safety
research. More recently, they have had a
significant impact in chemical-biological defense research. This symposium will describe efforts to
develop biosensors in which the biological material consists of cultured
cells/tissues or molecular probes. The
speakers will provide specific information on the use of biosensors in research
focused on detection of chemicals, biological toxins, or chemical signals of
explosive devices.
10:30 Introduction (W. J. Smith and G. Smagghe)
10:35 A-8 Challenges and
Future Directions for Detection of Ricin and Other
Related Toxins
Vipin
K. Rastogi, US Army
Edgewood Chemical Biological Center
11:00 A-9 Plants as Sensors
for Toxic Industrial Chemicals and Munitions: a Feasibility Analysis
Michael K. Deyholos, University of Alberta
11:25 A-10 Development of a Cell-based
Toxicity Sensor for Drinking Water Protection
Mark W. Widder, U.S. Army Center for Environmental Health Research
11:50 A-21 Fish Cell Lines as
Biosensors of Aquatic Environments
Lucila
E. J. Lee, Wilfrid Laurier University
12:15 Discussion
GENE EXPRESSION (NON-CODING
RNAS AND REGULATED TRANSGENE EXPRESSION)
Conveners: Glenn B. Collins, University of Kentucky,
Randy D. Dinkins, University of Kentucky, and Guiliang
Tang, University of Kentucky
10:30
am 12:30 pm Plant
Symposium..................... Grand
1
Gene expression,
including the transgene expression, is a multi-step
process that begins with gene transcription into messenger RNA (mRNA). Gene transcription and post-transcriptional
regulation are the two major regulatory steps in gene expression. Recent discovery of microRNAs
(miRNAs), a novel type of endogenous non-coding RNAs, and other endogenous small interfering RNAs (siRNAs) has pointed to a
novel level of gene regulation in plants and animals. These novel small non-coding RNAs are generated by an ancient pathway that is conserved
among higher eukaryotes and interact with their specific targets to down
regulate gene expression in a sequence-specific manner in three ways: (1)
targeting specific mRNAs for destruction (e.g., in plants); (2) targeting
specific mRNAs for translational suppression (e.g., in animals); and (3)
targeting specific chromatin for modification (in plants and animals). Transgene silencing
is directly related to these small RNAs. These small RNAs
are now implicated to play extensive roles in normal and abnormal growth and
development, including developmental timing, patterning, differentiation,
stress response, hormone signaling, and the development of various
cancers. Thousands of miRNAs have been identified in plants and animals and the
number is still increasing. It was
predicted that one third of the human genes are subjected to the regulation by miRNAs. In plants, miRNA target genes seem limited due to a high
complementarily between miRNAs and their target
genes, but these target genes are predominantly the genes encoding
transcription factors and other regulatory proteins. With the implication of a complex miRNA network, high throughput miRNA
array platforms have been developed and made it possible to monitor changes of
thousands of miRNAs in cells at any moment of growth
and development. The target genes of
these miRNAs are continuously revealed. It is expected that small non-coding RNAs will interweave into every field of plant science of
either fundamental or applied research.
10:30 Introduction (G. B. Collins, R. D. Dinkins, and G.
Tang)
10:35 P-7 Ecdysone Receptor Gene Switch Technology for Inducible Gene
Expression in Plants
Subba
Reddy Palli, University of Kentucky
11:10 P-8 Small RNA-pathways
in Plants
Allison Mallory, Laboratores de Biologie
Cellulaire
11:45 P-9 A Genome-wide View
of Small RNAs in Arabidopsis thaliana
Elisabeth J. Chapman, Oregon State University
12:20 Discussion
THE ACADEMIC INDUSTRY PARTNERSHIP:
TRAINING STUDENTS TO FILL A NEED
Convener: Elizabeth J. Roemer, SUNY Stony Brook, and Todd Murphy, Ivy Tech Community College
12:30
pm 2:00 pm Joint
Symposium....................... Capitol
The growth of
Pharmaceutical and Biotech industries has led to an ongoing need for a
scientifically literate and well-trained workforce. To address this need, academic institutions
across the country continue to develop multiple programs that range from the
2-year college to the graduate level. A
critical component in the success of such programs is communication between
industry: the future employers, and academia: the primary educators. This session will provide input from both
sides of that partnership with speakers from industry and academia.
12:30 Introduction (E. Roemer)
12:40 J-1 Biotechnology at
Ivy Tech: Designing a Degree Program in Partnership with Industry
Jose
Michael Hanquier, Eli Lilly and Company, and Todd W. Murphy, Ivy Tech Community College
Monday, June 11
Even Poster
Authors will be present
1:30 pm 2:30 pm
EUKARYOTIC CELLS FOR BIOTECHNOLOGY
AND CANCER BIOLOGY
Moderator: Lia H.
Campbell, Cell and Tissue Systems, Inc.
2:30
pm 3:30 pm Animal
Interactive Poster Session Grand 4
& 5
A-2000 Hepatocyte
Preservation as Spheroids
Lia
H. Campbell, Cell and Tissue Systems, Inc.,
Elizabeth D. Greene, and Kelvin G. M. Brockbank
A-2001 In Vitro Evaluation of Pertussis Toxin Composite Nanofibers
as a Non-invasive Whooping Cough Vaccine
Tejas
Gawade, Stony Brook
University, K. M. Sawicka, E. J. Roemer, and S. R.
Simon
A-2002 In
Vitro Spontaneous Transformation of Rat BDE1 Cholangiocytes Compared with Oncogenic
erbB-2/neu Transformants
Alphonese
E. Sirica, Virginia Commonwealth University, T. Asano, Z. Zhang, A. Mahatme, and D.
J. Ward
A-2003 In Vitro Effect of Carotenoids on Breast Cancer Cells
Paloma
Olvera-Caltzontzin, Universidad Autonoma de Queretaro, and T. Garcia-Gasca
A-2004 Prostaglandins A1 and E1 Influence
Gene Expression in an Established Insect Cell Line (BCIRL-HzAM1)
Cynthia L. Goodman, USDA/ARS/BCIRL, David Stanley, Qisheng Song, Shisheng An, and Arthur McIntosh
A-2005 In Vitro Generation of Amoebocytes: A New Source for Limulus Amoebocyte
Lysate Production
Amber Gail Brannan, Rose-Hulman Institute of Technology,
K. C. Dee, A. Buchmann, and W. W. Weiner
PLANT GENETIC ENGINEERING
FOR STRESS AND PEST RESISTANCE
Moderator: Maureen M. M. Fitch, USDA/ARS
2:30
pm 3:30 pm Plant
Interactive Poster Session Grand 4
& 5
P-2000 Chilling and Freezing Tolerance of
Transgenic Bahiagrass Over-expressing Structural and,
or Regulatory Genes Involved in Stress Protection
Jose M. Celedon, University of Florida IFAS, W. Fouad,
and F. Altpeter
P-2001 Greenhouse Screening and Field
Testing of Transgenic Grapevine for Fungal Resistance
Sadanand
A. Dhekney, University of Florida, Z. T. Li, M. Dutt, T. Zimmerman, and
D. Gray
P-2002 Transplastomic
Expression of the E. coli panD Enhances
Photosynthesis and Biomass Accumulation in Response to High Temperature Stress
Walid
M. Fouad, University of Florida, and F. Altpeter
P-2003 Screening of Transgenic Anthuriums for Bacterial Blight and Nematode Resistance
Maureen M. M. Fitch, USDA/ARS, T. Leong,
H. Albert, S. Schenck, P. Moore, H. McCafferty, J. Zhu, and D. Gonsalves
P-2004 Abiotic
Stress Tolerance of Bahiagrass with Expression of HvWRKY38 or OsMYB4 Transcription
Activators
Xi Xiong, University of Florida, V. James, and F. Altpeter
P-2005 Adventitious Shoot Regeneration
and Genetic Transformation of Prunus serotina for Reproductive Sterility
Xiaomei
Liu, Purdue University, Paula M. Pijut
P-2006 Promoters for Gene Expression in Developing
Fibers of Cultured Cotton Ovules
Jean L. Roberts, Dow AgroSciences
ENABLING TECHNOLOGIES FOR
CELL CULTURE TECHNOLOGY
Conveners: Lia H.
Campbell, Cell & Tissue Systems, Inc., and Linda B. Jacobsen, Roche Applied
Science
3:30
pm 5:00 pm Animal
Symposium.................... Grand
1
For decades,
cells, tissues or organs have been removed from the body and studied to gain
insight into biologic functions. As more
has been learned, existing techniques have been refined, optimized and applied
to answer more difficult questions while new techniques have been developed to
help answer these questions. This
session will focus on new technologies that now enable researchers to obtain
healthier cells for study, then examine the most
advanced technologies using flow cytometry and mass
spectroscopy that will enable researchers to answer key biologic questions that
could not previously be considered due to lack of appropriate technology.
3:30 Introduction (L. H. Campbell and L. B. Jacobsen)
3:35 A-11 New Look at an
Old Procedure: Tissue Dissociation in the 21st Century
Robert
McCarthy, VitaCyte
LLC
4:00 A-12 State-of-the-Art
in Flow Cytometry: 2007
J. Paul Robinson, Purdue University Cytometry Laboratories Bindley Bioscience Center
4:25 A-13 Ambient Mass
Spectrometry Applied to Biological Problems
R. Graham Cooks, Purdue University
4:50 Discussion
MICROPROPAGATION OF NOVEL CROPS FOR PHYTOCHEMICAL
PROPERTIES
Conveners: Jeffrey W. Adelberg,
Clemson University, and Nirmal Joshee,
Fort Valley State University
3:30
pm 5:00 pm Plant
Symposium..................... Grand
2
Most micropropagation involves ornamental and fruit crops. Medicinal, neutraceutical
and culinary herbs are receiving scientific attention for their health
promoting properties. Many of these are ferile or wild species, of limited geographic distribution,
and not readily available in commerce.
Few quality markers exist.
Mislabeled material, phyto-sanitary
restrictions, over harvest of scarce species, and local control of intellectual
property all place limits on scientific investigation. Developing quality standards, production,
harvest and extraction techniques, without degrading the native populations
often necessitates laboratory micropropagation. Exploring diversity within taxa of underutilized germplasm,
especially with slow growing perennial species, is facilitated by micropropagation.
Establishing farm-based enterprises for rearing plant material, without
spreading pathogens from distant lands, is well suited to micropropagation. Our speakers will share their experiences on
these issues bringing novel phytochemical crops to
utility.
3:30 Introduction (J. W. Adelberg
and N. Joshee)
3:35 P-19 Micropropagation as a Tool in Domestication of Medicinal
Crops
Rita M. Moraes, The University of Mississippi
4:00 P-20 Micropropagation of Novel Crops for Phytochemical
Properties
Hsin-Sheng Tsay, Chaoyang University of Technology
4:25 P-21 Spearmint
Plantlet Culture System as a Means to Study Secondary Metabolism In Vitro
Brent Tisserat, USDA
4:50 Discussion
LIFE SCIENCE BUSINESS START-UP
AND SURVIVAL
Convener: Dennis A. Laska,
Eli Lilly and Company, and David Jayme, Brigham Young University Hawaii
5:00 pm 6:00 pm Animal Symposium.................... Grand 1
Substantial
R&D investment by the pharmaceutical industry over the last twelve years
has led to a decrease in the introduction of new drugs onto the market. A white paper published in 2004 by the FDA
(Innovation or Stagnation) presented an analysis of the pipeline
problem. A key conclusion was that not
enough new tools are being developed to test the safety and effectiveness of
new products. Focus in this area of
critical path R&D will lead to decreased costs and reduced time for new
product development. Several leading pharmaceutical firms have tackled this
issue by looking outside to find innovative solutions to R&D problems.
Illustrations will be presented to highlight this shift, leading to the rise of
business ecosystems that are rapidly becoming an extended enterprise innovation
network. The implications of this change
for the viability of technically focused firms will be reviewed.
5:00 Introduction (D. A. Laska
and D. Jayme)
5:05 A-14 Outsourcing
Innovation in the Pharmaceutical Industry, Rise of Business Ecosystems
Robert
McCarthy, VitaCyte
LLC