WEDNESDAY, JUNE 7
7:00 am – 12:00 pm
Registration..........................Red Wing Room
ADVANCES IN PLANT TRANSFORMATION
Conveners:
Kan Wang, Iowa State University, and John J. Finer, Ohio State University
8:00 am – 10:05 am
Plant Symposium...................................Salon C
Plant Genetic Transformation is the essence of plant biotechnology and is critical for functional genomics studies. Although much
progress has been made in recent years using Agrobacterium-mediated transformation and physical delivery methods such as
particle bombardment, transformation efficiencies still need to be increased to meet the increasing demands in gene functional
analysis. In addition to simply generating more transgenics, high efficiency transformation will naturally lead to high throughput
functional analysis and recovery of higher quality transformants. This session will highlight some recent development in the area of
tissue culture and transformation methods, strategies for generating quality transformants, transgene expression tracking, and
evaluation of different nanotechnology tools for DNA delivery to plant cells. These advances in plant transformation could influence
the future direction of plant biology research.
8:00
Introduction (K. Wang and J. J. Finer)
8:05
P-31
High Efficiency and High Throughput Transformation of Cereals Mediated by Agrobacterium for Functional
Genomics
Toshihiko Komari, Japan Tobacco, Inc.
8:25
P-32
Plant Tissue Transformation Using Periodic Arrays of Vertically Aligned Carbon Nanofibers
Timothy Eric McKnight. Oak Ridge National Laboratory
8:45
P-33
DNA-coated Nanoparticles Mediated Transgene Expression in Plant Cells
François Torney, Iowa State University
9:05
P-34
Transfection: A Reliable and Efficient Method for Maize Transformation
Michael E. Horn, Phyton Biotech, Inc.
9:25
P-35
A Novel Plant Transformation Technology – Lipoic Acid
Yinghui Dan, Virginia Polytechnic Institute and State University-Blacksburg
9:45
P-36
Evaluation of an Automated Image Analysis System for Factors Which Stabilize Gene Expression
Joseph Chiera/John J. Finer, OARDC/The Ohio State University
EMBRYOGENESIS / REGENERATION / MICROPROPAGATION
Moderator:
Barbara M. Reed, USDA/ARS
8:00 am – 10:00 am
Plant Contributed Paper Session.........................
Duluth
8:00
P-1012
Abstract has been withdrawn
8:15
P-1013
Medium-term In Vitro Storage of Pear (Pyrus L.) Germplasm
Barbara M. Reed, USDA/ARS
8:30
P-1014
Indirect Shoot Organogenesis from Leaves of Dieffenbachia: A Method to Produce Somacolonal Variants
Xiu Li Shen, University of Florida, M. E. Kane, and J. Chen
8:45
P-1015
Micropropagation of Calopogon tuberosus, a Native Terrestrial Orchid, by Shoot Production from Corm
Explants
Philip Joseph Kauth, University of Florida, W. A. Vendrame, and M. E. Kane
9:00
P-1016
Comparative In Vitro Shoot Multiplication, Rooting, and Ex Vitro Acclimatization of Sea Oats (Uniola paniculata
L.) Genotypes
Michael E. Kane, University of Florida, N. L. Philman, P. Kauth, X. Shen, P. Sleszynski, S. Stewart, and C.
Valero-Aracama
9:15
P-1017
In Vitro Regeneration of Periwinkle
Andrea Swanberg, North Dakota State University, and Wenhao Dai
9:30
P-1018
Comparison of Different Callus and Plant Regeneration from Different Explants in Triploid and Tetraploid Turf-
type Bermudagrasses
Song Zhang, University of Georgia, Wayne Hanna, and Peggy Ozias-Akins
9:45
P-1019
The HBK3 Homeobox of Knox Gene is Responsible for Developmental Control and Shoot Apical Meristem
Determination in Maturing Somatic Embryos of Picea abies
Mark F. Belmonte, University of Manitoba, M. Tahir, and C. Stasolla
10:00 am – 10:30 am
Coffee Break
..........................Ballroom Foyer
DISTINGUISHED PLANT SYMPOSIUM
AGROBACTERIUM TRANSFORMATION: PAST, PRESENT, AND FUTURE
Convener:
Kan Wang, Iowa State University
10:30 am – 12:00 pm Distinguished Plant Symposium.................Salon C
The discovery of Agrobacterium tumefaciens and the use of its ability to deliver DNA into plant cells have practically redefined the
plant biology and agriculture today. In this plant keynote session, Professor Marc Van Montagu, the world renowned scientist and
the founding father (together with late Prof. Jeff Schell) of Agrobacterium-mediated transformation technology, will address the
audience with his experiences in the history of the discovery, his perspective on the present development of the technology and his
insight regarding the future of plant biology.
10:30
Introduction (K. Wang)
10:40
P-37
Agrobacterium Mediated Gene Transfer in Plants
Marc Van Montagu, Ghent University