TUESDAY, JUNE 6
7:00 am – 5:00 pm
Registration..........................Red Wing Room
CONTROLLING TRANSGENE DELIVERY AND INTEGRATION
Conveners:
Michael Bosela, Indiana University - Purdue University at Fort Wayne, and Theodore M. Klein,
Pioneer Crop Genetics
8:00 am – 10:00 am
Plenary Symposium.................................Salon C
Processes for delivering transgenes into cells and tissues have advanced dramatically in recent years. However, for most
eukaryotes, with the notable exception of yeasts and some specialized animal cell lines (e.g., murine embryonic stem
cells, etc.), DNA integration occurs primarily by illegitimate recombination at random locations in the genome. In contrast
with homologous recombination, where gene integration occurs at regions of DNA sequence homology, illegitimate
recombination is characterized by decreases in transgene fildelity (with truncation and transgene arrangements being
relatively common), more complex integration patterns (and associated increases in the frequency of homology
dependent gene silencing), and the possibility of collateral damage resulting from integration into gene coding sequences
or control elements (promoters, enhancers, etc). In addition, forward genetics and gene therapy (gene repair or
conversion) are only possible via homologous recombination. This session focuses on means to more precisely
manipulate transgene delivery; with the goal of increasing the degree of experimenter control over the location and type of
gene integration. Two approaches are emphasized. The first involves the use of heterologous or synthetic DNA
metabolism enzymes (e.g., recombinases, targeted endonucleases, etc) to stimulate site-specific integration and/or
homologous recombination. The second approach involves the development of artificial chromosomes, both as a means
to circumvent difficulties associated with random integration and as a platform for the delivery of large sets of genes. The
speakers will emphasize the applications of the new technologies and their utility across both plant and animals, and
model and non-model organisms.
8:00
Introduction (M. Bosela and T. M. Klein)
8:05
PS-6
Cre/lox Mediated Site-specific Gene Integration in Plants
Vibha Srivastava, University of Arkansas
8:30
PS-7
Homologous DNA Integration in Plants
Avraham A. Levy, The Weizmann Institute of Science, Israel
8:55
PS-8
Using Zinc-fingered Nuclease Mediated Homologous Recombination to Manipulate the Mammalian
Genome
Matthew H. Porteus, University of Texas
9:20
PS-9
Chromosome-based Gene Expression Platforms for Cell Engineering
Edward Perkins, University of Minnesota Medical School - Duluth
9:45
Discussion
10:00 am – 10:30 am
Coffee Break
.........................Salon D, E, G, F
ALTERNATIVE CROPS/USES
Conveners:
Nancy A. Reichert, Mississippi State University, and Margaret M. Young, Elizabeth City State
University
10:30 am – 12:30 pm
Plant Symposium...................................Salon C
There are thousands of plant species grown throughout the world, yet we rely on a select few to provide us with products
for input into primarily food/feed/fiber markets. Thinking outside the box, those “traditional” crops are being looked at for
input into alternative uses and markets. Also, plants not thought of as traditional “crops” are also being improved to
contribute value added benefits and products. This session will focus on research that could expand our definition of crops
and their uses.
10:30
Introduction
10:35
P-22
Expanding the Utility of Alfalfa
Richard A. Dixon, Samuel Roberts Noble Foundation
11:10
P-23
From Crops to Biorefineries
Olga V. Selifonova, Cargill, Inc.
11:45
P-24
TBA
Maurice M. Moloney, SemBioSys Genetics, Inc.
12:20
Discussion
COMMERCIALIZATION OF TRANSGENIC CROPS
Conveners:
David D. Songstad, Monsanto Company
10:30 am – 12:30 pm
Plant Symposium...................................Salon A
The commercialization of transgenic crops has now moved into its second decade and provides an opportunity to reflect
upon the progress and look forward to the future. Over the first decade, progress has been made toward the global
acceptance of transgenic crops with a cumulative planting of biotech crops now reaching one billion acres spanning 17
countries. In 2004 alone, 200 million acres of biotech crops were planted and it is projected that this will increase by 20%
for each of the 2005 and 2006 plantings. The future for transgenic crops looks very promising as the commercial climate
grows and expands into new crops and traits. In this session, presentations will cover both the progress made over the
first ten years of plant biotech crops and also the future as new crops and traits come to the marketplace.
10:30
Introduction
10:35
P-25
Development of Roundup Ready® Alfalfa Varieties
Mark McCaslin, Forage Genetics International
11:10
P-26
Development and Characterization of Alfalfa Populations Tolerant to Glyphosate
Glenn Rogan, Monsanto Company
11:45
P-27
Impact of Yieldgard Rootworm on Corn Rootworm Control
Scott C. Johnson, Monsanto Company
CRYOPRESERVATION
Convener:
Lia H. Campbell, Cell and Tissue Systems, Inc.
10:30 am – 12:30 pm
Animal Symposium
...................................
Duluth
Cryobiology is the study of cells at temperatures below the physiological temperature of 37
o
C. It encompasses many
aspects of preservation including hypothermic storage, cryopreservation, and freeze-drying among others. While most
researchers freeze cells, their experience with other aspects of cryobiology is limited. This symposium seeks to introduce
participants to other applications involving sub-physiological temperatures.
10:30
Introduction (L. H. Campbell)
10:35
A-14
Cryopreservation of Hepatocytes: Role of Culture Configuration on Survival
Allison Hubel, University of Minnesota
11:00
A-15
Biophysical and Molecular Changes Associated with Cryopreservation of Sperm
Ken Roberts, University of Minnesota Medical School
11:25
A-16
Thermal Injury Characterization for Biomedical Applications: In Vitro Model Systems
John C. Bischof, University of Minnesota
11:50
Discussion
1:30 pm – 2:30 pm
Odd Poster Authors will be present
....................................Salon D, E, G, F
ANIMAL CELL SCIENCES
Moderator:
Lia H. Campbell, Cell & Tissue Systems, Inc.
2:30 pm – 3:30 pm
Animal Interactive Poster Session.........Salon D, E, G, F
A-2000
Preservation of Cells by Freeze Drying
Lia H. Campbell, Cell & Tissue Systems, Inc., Kristy Sarver, Sarah Miller, Brian Leman, and Kelvin
G. M. Brockbank
A-2001
Use of a Cancer Cell Line Profiling Array to Evaluate the Effect of Chemotherapeutic Agents on Cullin
5 (Cul5) mRNA Expression
K. Zaffarkhan, Midwestern University, M. J. Fay, and C. Koch
A-2002
Calcium Oxalate Crystals in Commercial Fetal Bovine Serum: Implications for Cell Culture,
Phagocytosis and Biomineralization Studies In Vitro
Claudio E. Pedraza, McGill University, and M. D. McKee
A-2003
Antioxidant and Antiproliferative Effects of Turkish Rheum Ribes Ethyl Acetate Extract
Pembegul Uyar, Middle East Technical University
A-2004
In Vitro Tolerance of Filamentous Fungi to Environmental Pollutants: A Potential for Mycoremediation
Naiza Mae Natividad De Los Santos, University of the Philippines in the Visayas, Krysta Laureen
Palma, and Nelson D. Aclan
IN VITRO TOOLS, TECHNIQUES, AND OPTIMIZATION
Moderator:
Lindsey K. Tuominen, Orbital Technologies Corporation
2:30 pm – 3:30 pm
Plant Interactive Poster Session
..........Salon D, E, G, F
P-2019
Effects of Auxin Inclusion During Indirect Shoot Regeneration in Model Plant Species
Michael J. Bosela, Indiana University-Purdue University at Fort Wayne
P-2020
Time Course Study of Turmeric (Curcuma longa L.) Microrhizome Development in Large and Small
Vessels of Liquid Media with Varied PGR Concentrations
Jeffery Adelberg, Clemson University, and Matthew Cousins
P-2021
Miniature Sensors and LED Lighting for Advanced In Vitro Experimentation
Lindsey K. Tuominen, Orbital Technologies Corporation, D. J. Smith, A. G. Vermaak, J. C. Vignali,
M. J. Mischnick, and R. C. Morrow
P-2022
Germination and Plantlet Regeneration of Encapsulated Somatic Embryos from Transgenic Grape
(Vitis vinifera L.)
Niraj Kumar Nirala, Hamdard University, D. K. Das, M. K. Reddy, P.S. Srivastava, S. K. Sopory, and
K. C. Upadhyaya
P-2023
Identification and Cloning of RAPD Markers Linked to WA CMS in Rice (Oryza sativa L.)
Asadollah Ahmadikhah, Russian State Agricultural University, and G. I. Karlov
PLANT TRANSFORMATION
Moderator:
Maureen M. M. Fitch, USDA/ARS
2:30 pm – 3:30 pm
Plant Interactive Poster Session
..........Salon D, E, G, F
P-2006
Marker Gene Removal During Gene Transfer In Vitis spp.: A Technological Approach Toward an
Improved Science-society Communication
Lucia Martinelli, Istituto Agrario San Michele All’Adige, L. Dalla Costa, I. Vaccari, V. Poletti, and F.
Guzzo
P-2007
Transformation of Anthurium with Transgenes for Bacterial Blight and Nematode Resistance
Maureen M. M. Fitch, USDA/ARS, T. Leong, H. Albert, S. Schenck, P. Moore, and D. Gonsalves
P-2008
Genetic Transformation of Cyamopsis tetragonoloba (GUAR)
Sanchita Vaghchhipawala, The Samuel Roberts Noble Foundation, and Richard A. Dixon
P-2009
Agrobacterium-mediated Cotton Transformation and Regeneration-using Sucrose as Carbohydrate
Source and Selecting of Transgenics with Kanamycin Selection
Shubha Subbarao, Monsanto Company, J. Layton, N. Sidorova, L. Tan, J. Washam, E. Jakse, and
D. Duncan
P-2010
Development of an Efficient Agrobacterium-mediated Gene Transfer System for Multiple Sweetpotato
Cultivars
Jessica A. Scoffield, Tuskegee University, M. Egnin, B. Bey, M. Quain, C. S. Prakash, and D.
Mortley
P-2011
Assessment of Agrobacterium-mediated Transformation Methods: Simplifying the Induction Process
and Factors to Consider in Plant/Strain Competence Selection
Anwar A. Alsanabani, Alabama A & M University, Caula A. Beyl, and Anthony Ananga
PLANT BIOTECHNOLOGY
Moderator:
Charles Neal Stewart, Jr., University of Tennessee
3:15 pm – 5:00 pm
Plant Contributed Paper Session.......................Salon C
3:15
P-1020
Mineral Nutrient Requirements for Regulating the Growth of Plant Tissue
Randall P. Niedz, USDA-ARS, and T. J. Evens
3:30
P-1006
In Vitro Production of Turmeric (Curcuma longa L.) Microrhizomes as a Potential Source for
Secondary Metabolites
Matthew Cousins, Clemson University, Jeffrey Adelberg, Feng Chen, and James Rieck
3:45
P-1007
Castor Seed Development and Storage Lipid Biosynthesis
Grace Q. Chen, USDA/ARS, Yeh-Jin Ahn, and Louisa Vang
4:00
P-1008
Production of Biologically-active Acidothermus Cellulolyticus Endo-1, 4-â-gluconase (E1) Enzyme in
Transgenic Rice Plants for Alcohol Fuels and Cleaner Environment
Hesham Farouk Oraby, Michigan State University, Balan Venkatesh, Bruce Dale, Rashid Ahmad,
Callista Ransom, James Oehmke, and Mariam Sticklen
4:15
P-1009
High-yields and Extended Serum Half-life of Therapeutic Proteins Expressed as Fusion Glycoproteins
in Tobacco Cells
Jianfeng Xu, Ohio University, S. Okada, K. J. Goodrum, J. J. Kopchick, and M. J. Kieliszewski
4:30
P-1010
Plant Transformation and Horizontal Gene Flow of a Plant ABC Transporter Gene
Charles Neal Stewart, Jr., University of Tennessee, R. J. Millwood, J. S. Davis, K. P. Burris, A.
Mentewab, and J. N. Burris
4:45
P-1011
Quantitative Multiplex Real-time PCR as a Screening Tool for Estimating Transgene Copy Number in
Transgenic Citrus
Ahmad Al-Sayed Omar, University of Florida, M. G. H. Dekkers, J. H. Graham, and J. W. Grosser
LINKING SPECIES FOR GENOME ANALYSIS
Conveners:
Peggy J. Ozias-Akins, University of Georgia, and Heidi F. Kaeppler, University of Wisconsin
3:30 pm – 5:00 pm
Plant Symposium...................................Salon A
Genomics research benefits greatly from cross-species comparison for sequence characterization, genetic dissection and
functional analysis of genomes. Although species often are distinguished by their inability to mate, sexual compatibility is
no longer a barrier to linking genomes. In this symposium, various interpretations of “linking” species will be addressed.
These include the development and application of radiation hybrids containing chromosomes mainly of one species but
being distinguished by the presence of a chromosome from a different species whose similarities promote analysis but
whose differences allow characterization that could not be accomplished in its own genetic background. Another
application is derived from the discovery that genomic similarities among species are extensive. These similarities have
been useful for genetic mapping and gene isolation as well as expanding our understanding of species evolution.
3:30
Introduction
3:35
P-28
Wide-cross Whole-genome Radiation Hybrid Mapping in Cotton
David M. Stelly, Texas A&M University
4:00
P-29
Evolution of Chromatin Structure and Function
Shawn M. Kaeppler, University of Wisconsin – Madison
4:25
P-30
Allium Genomics: Exploiting Model Plants for Analyses of Enormous Nuclear Genomes
Michael J. Havey, University of Wisconsin – Madison
4:50
Discussion
THREE-DIMENSIONAL TISSUE MODELS OF CANCER CELL INVASION AND
METASTASIS
Convener:
Jonathan Garlick, Tufts University
3:30 pm – 5:00 pm
Animal Symposium
...................................
Duluth
It is now clear that the tumor microenvironment plays an essential role in all stages of cancer progression. This is based
on observations showing that cancer cells demonstrate complex cross-talk with the surrounding stroma and with its
constituent cells during invasion and metastasis. In this light, cancer development is dependant on reciprocal interactions
regulated by the manner in which cancer cells communicate with neighboring cells, with adjacent stroma and with soluble
growth factors. As a result, it is very important that experimental paradigms designed to study these processes manifest
these complex cellular interactions as they occur in vivo. In recent years, three-dimensional (3D) human tissue models
that mimic various stages of cancer progression have been developed to significantly advance our ability to study cancer
in a biologically-meaningful tissue context. The goal of this symposium is to provide an overview and update on studies
using these human, 3D experimental models that have been used to study how the tumor microenvironment regulates
invasion and metastasis during cancer development. This will be accomplished through three presentations that will
describe 3D, human tissue models that have revealed mechanisms through which defined stages of breast cancer,
squamous cell carcinoma and melanoma are controlled by signals from the tumor microenvironment.
3:30
Introduction
3:35
A-17
Three-dimensional Microenvironment and Breast Cancer Progression
Penney Gilbert, University of Pennsylvania
4:00
A-18
3D In Vitro Models Reveal the Invasive, Drug Resistant Phenotype of Metastatic Melanoma
Keiran Smalley, The Wistar Institute
4:25
A-19
Reconstructing and Deconstructing the Progression of Human Squamous Cell Carcinoma in 3D
Tissue Models
Addy Alt-Holland, Tufts University
4:50
Discussion
5:00 pm – 5:45 pm
SIVB Business Meeting ........................Rochester
(All Members are Urged to Attend)
6:00 pm – 10:00 pm
An Evening at the Mill City Museum Event..................................................
Mill City Museum
Buses Depart at 5:45
(Admittance by Advance Ticket Holders Only)