SUNDAY, JUNE 4
7:00 am – 6:00 pm
Registration..........................Red Wing Room
MOLECULAR NUTRITION
Conveners:
Jeffrey W. Adelberg, Clemson University, and Linda B. Jacobsen, Roche Applied Science
8:00 am – 10:00 am
Plenary Symposium.................................Salon C
All life started in the sea and we still have saline solutions bathing all vital tissues. The concentration and
organic complexation of nutrients confers specificity for their physiological roles. Higher plants are non-motile
and meet environmental challenges with altered enzyme responses. Nutritional and defense compounds from
plants can have similar functions in regulating heterologous systems found in plants and animals. In vitro
systems are a powerful tool to ask vital questions on how chemicals become nutrients, and how nutrients affect
the target organism. It is known that animal cells directly or indirectly concentrate their nutrients from plants and
require more concentrated nutrients than plant cells. Thus key to understanding the total picture is the
identification of chemicals/nutrients in culture media that are required by animal cells to reproduce and function.
8:00
Introduction (J. Adelberg and L. Jacobsen)
8:05
PS-1
Advances in Molecular Nutrition: Phytochemicals and Antioxidant Response Pathways as
Critical Control Points for Chemoprevention of Disease
Kalidas Shetty, University of Massachusetts
9:00
PS-2
Genomic and Proteomic Approaches to Understanding and Manipulating Nutritional
Requirements in Mammalian Cell Culture Processes
Laurel Marie Donahue, SAFC Biosciences
10:00 am – 10:30 am
Coffee Break
.........................Salon D, E, G, F
CELL-BASED BIOASSAYS AS ESSENTIAL TOOLS IN DRUG
DEVELOPMENT
Convener:
John W. Harbell, Mary Kay Inc.
10:30 am – 12:00 pm
Animal Symposium
...................................
Duluth
Cell-based bioassays have become a mainstay in drug discovery and development. These assays range from
simple screens for basic toxicity or efficacy to systems involving multiple cell types and a complex array of end
points. Even the most “simple” of these assays involves a series of independent variables that must be defined
and controlled in the study design. Factors such as the selection of the target cells, cell number, duration of
exposure, and endpoints will impact the resulting data and predictive capacity of the test. An understanding of
the design and validation processes that have gone into successful bioassays is key to the design and
validation of future assays. This symposium will present examples of three successful and large scale bioassay
programs.
10:30
Introduction (J. Harbell)
10:35
A-1
The NCI 60 Tumor Cell Line Screen: An Information-rich Screen Informing on Mechanisms
of Toxicity
Robert H. Shoemaker, National Cancer Institute
11:00
A-2
High-fidelity In Vitro Modeling of Clinically-defined, Chemically-induced Organ Toxicity in
Man Using Adverse Effects of Oncology Drugs as Ethical Learning Sets
Ralph E. Parchment, NCI-Frederick Cancer Research Facility
11:25
A-3
Assessing Hepatotoxicity Through Multiple Endpoints
James M. McKim, CeeTox, Inc.
FUNCTIONAL GENOMICS
Conveners:
Baochun Li, University of Kentucky, and Allan R. Wenck, BASF Plant Sciences
10:30 am – 12:30 pm
Plant Symposium...................................Salon C
Studying the function of plant genes and developing new tools for functional genomics studies are of
fundamental interest and advances in this field are being made at a very dramatic pace. The speakers from this
session will update their research on the following areas: molecular mechanisms that govern plant development
and floral induction; molecular mechanisms that underlie plant responses to harsh environment such as soil
salinity, drought and cold temperatures; small RNA pathways in plants; and a highly efficient Agrobacterium-
tumefaciens-mediated transient gene expression system using TMV-based binary vectors.
10:30
Introduction (B. Li and A. Wenck)
10:35
P-1
Remembering Winter: Vernalization as an Environmentally Induced Epigenetic Switch
Richard M. Amasino, University of Wisconsin
11:00
P-2
Role of miRNAs and siRNAs in Abiotic Stress Repsonses
Jian-Kang Zhu, University of California
11:25
P-3
Diverse Small RNA-directed Pathways in Plants
Zhixin Xie, Texas Tech University
11:50
P-4
High Throughput Gene Assembly and Expression Using Viral RNA Replicons Delivered by
Agrobacterium
Yuri Gleba, Icon Genetics
12:15
Discussion
GENOMIC APPROACHES TO HOST-PATHOGEN INTERACTIONS
Convener:
Gary Muehlbauer, University of Minnesota
10:30 am – 12:30 pm
Plant Symposium
Salon A
Plant pathogens cause significant economic damage to the agricultural production worldwide by direct reduction
of yields and losses of market and export. How do plant pathogens infect host plants and establish disease
interactions with host plants? What are the host responses and gene expression networks induced during
infection? The science of genomics is leading to an advanced understanding of how genes function in a
coordinate fashion that results in various organismal characteristics or phenotypes. Functional, structural, and
comparative genome analysis of plant pathogens and their hosts will provide insights to understand the
questions. These understandings are essential to the development of effective and environmentally sound
strategies for disease control. This symposium will provide an update on host-pathogen interactions.
10:30
Introduction (G. Muehlbauer)
10:35
P-5
Signaling Networks Controlling Disease Resistance Responses in Arabidopsis
Jane Glazebrook, University of Minnesota
11:00
P-6
Profiles in Scourge: Gene Expression Analysis of a Crop Killer
H. Corby Kistler, USDA-ARS
11:25
P-7
In Planta Transcriptional and Functional Patterns of an Agriculturally Relevant R Gene
James M. Bradeen, University of Minnesota
11:50
P-8
Use of a High-performance, Custom Microarray for Elucidation of Signaling Networks
Controlling Plant Defense Responses
Fumiaki Katagiri, University of Minnesota
12:15
Discussion
PLANT BIOTECHNOLOGY AFTER THE FARMERS’
FIELDS – TESTING, TRACKING, AND ECONOMICS
Convener:
Raymond D. Shillito, Bayer CropScience
1:30 pm – 3:00 pm
Plant Symposium...................................Salon B
Once a transgenic has been commercialized, many economic issues affect its acceptability and adoption. Plant
varieties must meet performance requirements - so tests are needed for ensuring purity. Once the crop leaves
the farm gate it may be segregated for use in certain markets. Thus testing is needed in order to satisfy the
needs of those involved in trade of grain and foodstuffs to comply with regulatory and labeling requirements.
The segregation and testing of grain and food and feed for the presence or absence of transgenic crop products
can have major economic impacts. This session will describe the types of tests available for detecting and
quantifying transgenic seeds and grain, describe how these are applied in the grain industry, and look at the
economic issues raised by these testing regimes.
1:30
Introduction (R. Shillito)
1:35
P-9
Testing Methods for DNA and Proteins in Transgenic Crops
Raymond D. Shillito, Bayer CropScience
2:00
P-10
Applications of Testing Methods in the Grain Industry
Randal Giroux, Cargill. Inc.
2:25
P-11
A Global Perspective on the Economic Impact of Transgenic Crop Varieties
Greg Traxler, Auburn University
PLANT TRANSFORMATION
Moderator:
Dennis J. Gray, University of Florida
1:30 pm – 3:00 pm
Plant Contributed Paper Session.......................Salon A
1:30
P-1000
RNAi-mediated Silencing of Maize Chromatin Genes Confer Increased Transformation
Efficiency in Maize
Mary Ann McGill, University of Wisconsin-Madison, S. M. Kaeppler, and H. F. Kaeppler
1:45
P-1001
Stable Transformation of Taxus
Helena Mathews, Exelixis Plant Sciences, Nikolaus Matheis, Fira Negru, Karin Connors,
Vaka Reddy, Al Lammers, Debra Schuster, Mylavarapu Venkatramesh, and D. Ry Wagner
2:00
P-1002
A Novel Shoot Organogenesis and Transformation System for Nicotiana obtusifolia
Accession PI 555573
Baochun Li, University of Kentucky, Qingwei Huang, and Hui Qiu
2:15
P-1003
Selecting Disease Resistant Transgenic Grapevine for Field Tests
Dennis J. Gray, University of Florida, Z. T. Li, S. A. Dhekney, M. Dutt, M. Van Aman, J.
Tattersall, and K. T. Kelley
2:30
P-1004
The Immunodominant Allergan Ara h2 is Silenced in Transgenic Peanut Via the RNA
Interference (RNAi) Strategy
Koffi N. Konan, Alabama A&M University, O. M. Viquez, and H. W.
Dodo
2:45
P-1005
Antibiotic Marker Free Approach for Obtaining Salt Stress Tolerant Vigna mungo
(Blackgram)
Neera Bhalla Sarin, Jawaharlal Nehru University, P. Bhomkar, C. P. Upadhyay, S.
DebRoy, R. Rajwanshi, A. Muthusamy, M. Saxena, N. Shiva Prakash, M. Pooggin, and T.
Hohn
RECENT ADVANCES IN INVERTEBRATE IMMUNITY USING IN VITRO
TECHNIQUES
Conveners:
Cynthia L. Goodman, USDA/ARS/BCIRL, and Guy Smagghe, Ghent University
1:30 pm – 3:00 pm
Animal Symposium
...................................
Duluth
Understanding and manipulating insect immunity has important implications for the control of agriculturally and
medically important insects, including lepidopterans that destroy crops as well as dipterans that vector human
and animal diseases. The innate immune system in insects is quite complex, with both humoral and cellular
components. By using cell and tissue culture techniques, these components can be isolated and their
mechanisms and specific effects more directly studied (Fallon and Sun 2001, Insect Biochem. Molec. Biol.
31:263). One aspect of immunity that can be readily investigated using in vitro methods is the identification of
the signaling pathways involved in controlling the various immune responses. Eicosanoids (Stanley 2006,
Annu. Rev. Entomol. 51: 25-44) and insect hormones (Franssens et al., Dev. Comp. Immunol., submitted) play
important roles in this arena. To summarize, this symposium will discuss a number of important topics related
to insect immunity and the in vitro methodologies used to dissect them, with potential field applications of these
studies also being noted.
1:30
Introduction (C. Goodman and G. Smagghe)
1:35
A-4
Eicosanoids in Invertebrate Immunity: An In Vitro Approach
David Stanley, USDA/ARS/BCIRL
2:00
A-5
Experimental Approaches to the Evaluation of Immune Functions in Mosquito Cell Lines
Ann M. Fallon, University of Minnesota
2:25
A-6
Determination of the Effects of Ecdysteroids and JH on Nodulation Responses
Vanessa Franssens, University of Leuven
2006 IN VITRO BIOLOGY OPENING CEREMONY
Program Chair:
Mark C. Jordan, Agriculture and Agri-Food Canada
Program Co-Chairs:
Guido F. Caputo, Canadian Forest Service
John W. Harbell
3:00 pm – 5:30 pm
Opening Ceremony.................................Salon C
Conveners:
David W. Altman, IPR Consulting, Inc
Mark C. Jordan, Agriculture and Agri-Food Canada
Paul J. Price, Invitrogen
3:00
Welcome and Opening Remarks:
David W. Altman, SIVB Past President, Society for In Vitro Biology
Paul J. Price, President, Society for In Vitro Biology
3:10
2006 Fellow Award Recipients (Awards to be presented at Section Meetings):
Guido F. Caputo, Canadian Forest Service
Dennis A. Laska, Eli Lilly & Company
Shirley A. Pomponi, Harbor Branch Oceanographic Institution
Nancy A. Reichert, Mississippi State University
Amy A. Wang, GlaxoSmithKline
3:20
2006 Distinguished Service Award Presentations:
Delia R. Bethell, Ventria Bioscience
Nancy A. Reichert, Mississippi State University
David D. Songstad, Monsanto Company
3:30
2006 Lifetime Achievement Award Recipients:
Bob V. Conger, University of Tennessee
(Introduction presented by David D. Songstad)
Wei-Shou Hu, University of Minnesota
(Introduction presented by David W. Jayme)
4:00
Keynote Symposium
Introduction (D. W. Altman and M. C. Jordan)
KS-1
Ever-expanding Horizons
Ronald L. Phillips, University of Minnesota
5:30 pm – 7:30 pm
2006 IN VITRO BIOLOGY MEETING
OPENING CEREMONY RECEPTION .................................Salon D, E, G, F
5:30 pm – 7:30 pm
Exhibits and Posters
...................Salon D, E, G, F
6:30 pm – 7:30 pm
Odd Poster Authors will be present
....................................Salon D, E, G, F