MONDAY, JUNE 5
7:00 am – 6:30 pm
Registration..........................Red Wing Room
BIOREACTORS: APPLICATION OF CULTURED EUKARYOTIC CELLS FOR
BIOPHARMACEUTICAL PRODUCTION
Conveners:
Michael E. Horn, Phyton Biotech, Inc., and David Jayme, Brigham Young University – Hawaii
8:00 am – 10:00 am
Plenary Symposium.................................Salon C
There often exist multiple options for the production of biopharmaceuticals, many of them involving eukaryotic cell culture
processes. Process development requires comparative analysis of these options and appreciation of their relative
strengths and limitations to project the superior route for bioproduction. This plenary session invites speakers
experienced with the production of biological compounds in vertebrate, invertebrate and plant cell-based cultures. Using
case studies from their personal experiences, they will explore the utility of their respective culture systems to achieve
bioproduction objectives.
8:00
Introduction (M. E. Horn and D. Jayme)
8:05
PS-3
Animal Cell Culture: The Future Beyond Bioreactors and Metabolic Engineering
Wei-Shou Hu, University of Minnesota
8:40
PS-4
Baculovirus Recombinant Protein Production in Insect and Mammalian Cells
Thomas A. Kost, GlaxoSmithKline
9:15
PS-5
Commercial Production of Paclitaxel by Plant Cell Culture
Venkatesh Srinivasan, Phyton Biotech, Inc.
9:50
Discussion
10:00 am – 10:30 am
Coffee Break
.........................Salon D, E, G, F
3D CELL CONSTRUCTS FOR TISSUE ENGINEERING
Convener:
Robert Tranquillo, University of Minnesota
10:30 am – 12:00 pm
Animal Symposium
...................................
Duluth
The success of tissue engineering hinges on an appropriate combination of cells, polymer scaffolds, and culture
conditions, often involving controlled mechanical and chemical signals. This session will provide state-of-art studies
among a range of key applications that explore these critical factors.
10:30
Introduction (R. Tranquillo)
10:35
A-7
Multipotent Adult Progenitor Cells for Vascular Repair
Catherine M. Verfaillie, University of Minnesota
11:00
A-8
Cell Sourcing for Fibrin-based Heart Valve-equivalents
Chrysanthi Williams, Bose Corporation
11:25
A-9
Engineering Large, Mineralized Bone Tissue Constructs Using Human Mesenchymal Stem Cells
Gordana V. Vunjak-Novakovic, Columbia University
GLOBAL COMMERCIAL MICROPROPAGATION: CHALLENGES AND
OPPORTUNITIES
Conveners:
Michael E. Kane, University of Florida, Michael Becwar, ArborGen LLC, and Jeffrey W. Adelberg,
Clemson University
10:30 am – 12:30 pm
Plant Symposium...................................Salon B
Micropropagation, the application of tissue culture for efficient clonal plant production, has been used commercially since
the 1960’s and is possibly the oldest example of commercial biotechnology. In the United States, the micropropagation
industry has developed primarily to service the temperate and tropical ornamental plant industry. Production of elite stock
plants for small fruit and vegetable crops is a secondary area of activity. Clonal propagation of coniferous forest trees via
somatic embryogenesis is being pursued for large-scale production in the US. Manufactured seed and automation
technologies in combination offer a cost effective method of producing, selecting and deploying somatic embryos into
conifer nurseries. Tropical plantation crops, outside this country have been commercially micropropagated for many
years. Recently, bioreactor technology has been implemented for efficient large-scale propagation of several fruit and
timber crops. Our speakers will provide differing perspectives on the critical challenges and technology developments
that impact this industry.
10:30
Introduction (M. E. Kane, M. Becwar, and J. W. Adelberg)
10:35
P-12
Current Status and Impact of Commercial Plant Tissue Culture
Steve McCulloch, Mountain Shadow Nursery
11:00
P-13
Status of Commercial Tropical Foliage Plant Micropropagation
Gary Hennen, Oglesby Plants International, Inc.
11:25
P-14
Temporary Immersion Bioreactor: An Efficient Technology for Scaling-up Plant Production
Maritza Escalona Morgado, University of Ciego de Avila
11:50
P-15
Manufactured Seed - An Efficient Method for Delivery of Somatic Embryos to Nurseries
William C. Carlson, Weyerhaeuser
12:15
Discussion
PLANT-MADE PHARMACEUTICALS
Convener:
Delia R. Bethell, Ventria Bioscience
10:30 am – 12:30 pm
Plant Symposium...................................Salon A
The production of protein therapies using plants as the expression system is gaining steady acceptance. The technology
is moving beyond the theoretical and the lab bench and into the clinic. This session will explore the progress being made
in the clinical application of plant made proteins and pharmaceuticals.
10:30
Introduction (D. Bethell)
10:35
P-16
Therapeutic Protein Expression in the Plant-based LEX System™
Vincent Wingate, Biolex
11:10
P-17
Production of Biodefense-related Proteins in Tobacco
Keith Wycoff, Planet Biotechnology, Inc.
11:45
P-18
Transgenic Expression and Recovery of Biologically Active Recombinant Human Insulin from
Arabidopsis thaliana Oilseeds
Elizabeth W. Murray, SemBioSys Genetics, Inc.
12:20
Discussion
1:30 pm – 2:30 pm
Even Poster Authors will be present
....................................Salon D, E, G, F
ANIMAL CELL SCIENCES
Moderator:
Eugene Elmore, University of California – Irvine
2:30 pm – 3:30 pm
Animal Contributed Paper Session
.......................
Duluth
2:30
A-1000
Response of Rosiglitazone, UAB 30, and Atorvastatin in the Human Melanoma Prevention Assay
Eugene Elmore, University of California – Irvine, A. Jain, L. Kopelovich, F. L. Meyskens, V. E.
Steele, and J. L. Redpath
2:45
A-1001
Lineage Specificity and Interspecies Variation in Hematopoietic Toxicity Testing
Cindy Miller, StemCell Technologies, Inc., Carla Pereira, Jackie Damen, and Emer Clarke
3:00
A-1002
Computer-aided Tissue Engineering: Predicting Self-assembly of Prostate Cancer Spheroids
Kim C. O’Connor, Tulane University, H. Song, and S. Clejan
3:15
A-1003
Characterization of Neuroblastoma Cells Cultured in Three-dimensional, Microgravity Rotary
Bioreactor: Organoid Formation and Free Cell Dynamics
Robert Asbury Redden, The Children’s Hospital of Philadelphia, and E. J. Doolin
EMBRYOGENESIS/ REGENERATION/ MICROPROPAGATION
Moderator:
Vicki Magnusson, North Dakota State University
2:30 pm – 3:30 pm
Plant Interactive Poster Session
..........Salon D, E, G, F
P-2012
In Vitro Propagation of Silver Maple
Vicki Magnusson, North Dakota State University, and Wenhao Dai
P-2013
In Vitro Specificity Exhibited by Fungal Mycobionts of Spiranthes floridana (Orchidaceae) within the
Congener Spiranthes brevilabris
Scott L. Stewart, University of Florida, and M. E. Kane
P-2014
Optimization of Regeneration in Pongamia pinnata (L.) Pierre - a Potential Biodiesel Plant Using
Taguchi Approach
B. Srinivas, Sreenidhi Institute of Science and Technology (SNIST), and S. Ramgopal Rao
P-2015
Callus Establishment and Shoot Proliferation in Jatropha curcas: A Biodiesel Plant Through Nodal
Explant Culture
Shailendra Kumar Tiwari, Plant Propagation & Biotechnology Division State Forest Research
Instituite, P. K. Shukla, Amit Pandey, and M. P. Goswami
P-2016
Recovery from Gross Apical Damage in Dicotyledonous Seedlings
Victor Gaba, ARO Volcani Center, L. Jashi, S. Amutha, K. Kathiravan, S. Singer, I. Shomer, and B.
Steinitz
P-2017
In Vitro Propagation of Rare and Disappear Plants
Magfrat P. Muminova, Institute of Genetics & PE BAS of Uzbekistan, O. I. Isaeva, I. N. Grigina, and
S. S. Khamrakulov
INVERTEBRATE
Moderator:
Guido Caputo, Canadian Forest Service
2:30 pm – 3:30 pm
Invertebrate Interactive Poster Session
Salon D, E, G, F
I-2000
Eicosanoids Influence Insect Cell-viral Interactions
Cynthia L. Goodman, USDA/ARS/ BCIRL, A. McIntosh, and D. Stanley
I-2001
Stable Transformation of a Tick (Ixodes scapularis) Cell Line with the Sleeping Beauty Transposon
System
Timothy J. Kurtti, University of Minnesota, R. F. Felsheim, J. T. Mattila, G. D. Baldridge, N. Y.
Burkhardt, and U. G. Munderloh
I-2002
RNAi-mediated Silencing of a DsRed2-expressing Ixodes Scapularis (Acari: Ixodidae) Cell Line
Joshua T. Mattila, University of Minnesota, G. D. Baldridge, R. F. Felsheim, N. Y. Burkhardt, U. G.
Munderloh, and T. J. Kurtti
I-2003
Electroporation of Crithidia ricardoi with pNUS-GFPH
Moses A. McDaniel, Elizabeth City State University, Gieira Jones, Margaret M.Young, Gary L.
Harmon, and Ronald H. Blackmon
I-2004
Functional Analysis of Nictaba in Insect Midgut Cells
Guy Smagghe, Lab Agrozoology, Ghent University, G. Vandenborre, T. Soin, L. Jacobsen, G.
Caputo, and E. J. M. Van Damme
I-2005
Validation Analysis of an Ecdysteroid Receptor Agonist Assay Using Intact Cultured Lepidoptera
Cells
Guy Smagghe, Lab Agrozoology, Ghent University, H. Mosallanejad, L. Decombel, C. Goodman,
and T. Soin
I-2006
Biologically Potent Broad Spectrum Antibiotics Obtained from the Tetrodotoxin Rich Organs of Puffer
Fishes
Joey D. Mangadlao, University of the Philippines
SECONDARY PRODUCTS AND BIOTECHNOLOGY
Moderator:
Mitchell L. Wise, USDA/ARS
2:30 pm – 3:30 pm
Plant Interactive Poster Session
..........Salon D, E, G, F
P-2000
Elicitation of Pueraria lobata (Kudzu) Cell and Root Cultures for Radiolabeling of Isoflavones
Nelson Adam Reppert, University of Illinois Urbana-Champaign, G. Yousef, R. B. Rogers, and M. A.
Lila
P-2001
Copper Chloride Elicitation of In Vitro Red Clover Isoflavones
Nancy Engelmann, University of Illinois, Urbana-Champaign, Randy Rogers, Padmapriya Vattem,
Jeevan Prasain, and Mary Ann Lila
P-2002
Biosynthesis of Avenanthramides in Chitin Elicited Oat (Avena sativa) Suspension Cultures
Mitchell L. Wise, USDA/ARS
P-2003
Cell-free Expression of the Tobacco Lectin
Guy Smagghe, Ghent University, D. Breite, E. Daniel, G. Vandenborre, N. Lannoo, L. Jacobsen, and
E. J. M. Van Damme
P-2004
Rapid Screening of Silent Mutations for Cell-free Production of the Plant Lectin nictaba
Erica Daniel, Indiana University, D. Breite, L. Jacobsen, E. J. M. Van Damme, and G. Smagghe
P-2005
Tissue Culture of Nerium oleander Possesses Cytotoxic Activity for Human Cell Lines In Vitro
Nelli A. Hovhannisyan, Yerevan State University
BIOINFORMATICS TOOLS FOR BIOTECHNOLOGISTS
Conveners:
Peggy J. Ozias-Akins, University of Georgia, and Sylvia Adjoa
Mitchell, University of the West Indies
3:30 pm – 5:00 pm
Plant Symposium...................................Salon C
Bioinformatics combines aspects of the biological sciences with information technology and thereby provides a means to
store and mine complex biological data sets. Databases are essential components of bioinformatics and should be
carefully designed in order to optimize subsequent mining through queries. This workshop is intended for the novice as
an introduction to the design concepts of relational databases, the computational environments and programming skills
that are required, and the potential applications to biotechnology.
3:30
Introduction (P. J. Ozias-Akins and S. A. Mitchell)
3:35
TBD
Zheng Jin Tu, University of Minnesota
CONSERVING ENDANGERED ORCHIDS: AN IN VITRO PERSPECTIVE
Conveners:
Valerie C. Pence, Cincinnati Zoo and Botanical Gardens, and Barbara M. Reed, USDA/ARS
3:30 pm – 5:00 pm
Plant Symposium...................................Salon A
Conservation efforts to preserve rare and endangered plants involve both in situ preserves and ex situ collections. In vitro
culture of rare orchids can provide a secure storage form for these popular flowers as well as providing specimens for
population augmentation, reintroduction, and establishment. This symposium will include several perspectives on the use
of in vitro culture to preserve and protect rare and endangered orchid species.
3:30
Introduction (V. C. Pence and B. M. Reed)
3:35
P-19
An Overview of the Orchid-fungal Sybiosis in Nature, and Its Application In Vitro to Promote
Conservation
Lawrence W. Zettler, Illinois College
4:00
P-20
Symbiotic and Asymbiotic Orchid Seed Germination as Tools in Conservation
Scott L. Stewart, University of Florida
4:25
P-21
In Vitro Strategies for Conservation of Madagascar’s Endemic Orchids
Margaret Mary From, Omaha’s Henry Doorly Zoo
4:50
Discussion
STEM CELLS
Convener:
Gordana V. Vunjak-Novakovic, Columbia University
3:30 pm – 5:00 pm
Animal Symposium
...................................
Duluth
Stem cells have the potential for self-renewal and the potential for differentiation into any specific cell lineage. Our ability
to utilize this unique potential depends on in vitro methods that can recapitulate some of the conditions present during
normal development and thereby regulate stem cell differentiation. Focus of this session is on stem cells, in the context of
their application in tissue engineering and regenerative medicine.
3:30
Introduction (G. V. Vunjak-Novakovic)
3:35
A-10
Stem Cell Based Artificial Heart
Doris Taylor, University of Minnesota
4:00
A-11
Blood and Endothelial Cell Development from Human Embryonic Stem Cells
Dan S. Kaufman, University of Minnesota
4:25
A-12
Muscle Stem Cell: Satellite Cell and Sca-1-positive Cell
Atsushi Asakura, University of Minnesota Medical School
4:50
Discussion
NUTRITIONAL REQUIREMENTS OF MAMMALIAN CELLS IN CULTURE DESIGN
AND OPTIMIZATION OF THE CELL CULTURE MEDIUM AND PROCESSES
Convener: Paul
J. Price, GIBCO Invitrogen
5:00 pm – 6:00 pm
Animal Symposium
...................................
Duluth
A classical cell culture medium is made up of a buffered isotonic salt solution supplemented with vitamins, amino acids, a
source of energy such as glucose or glutamine and a protein supplement such as serum. As the science of media
formulation progressed from serum supplemented to serum-free and then to chemically-defined, substitutes for the serum
component and then for serum derived proteins had to be found. Many segments of Industry are presently moving away
from media and reagents containing any component of animal or human origin. The outbreak of BSE and CJV in England
heightened the awareness of the risk of contamination of therapeutics by prions or viruses and prompted regulatory
pressure for companies to address this potential risk. This teaching seminar will cover the basics of a cell culture medium
and the advantages and disadvantages of the serum supplement. It will then progress into the construction of serum-free
and chemically defined formulations and then to the elimination of all components of animal origin. Ways to optimize both
cell growth and productivity will be presented as well as the design of media for specific applications. Emphasis will be
placed on reducing apoptosis by controlling osmolality, ammonia, and free radical production and through optimization of
the media formulation.
5:00
A-13
Nutritional Requirements of Mammalian Cells in Culture: Design and Optimization of the Cell Culture
Medium and Processes
Paul J. Price, GIBCO Invitrogen
STUDENT PROFESSIONAL DEVELOPMENT
Moderator:
Mary Ann McGill, University of Wisconsin
5:00 pm – 6:00 pm
Joint Symposium...................................Salon A
One of the most beneficial aspects of the SIVB meetings is the chance to interact with researchers from academic,
industry, and government institutions. As students get closer to graduating, they start to ask themselves questions like
“academia or industry?” or “If I go to industry without doing a postdoc, can I come back to an academic institution?” and
other questions of that nature. This workshop will feature a discussion panel with representatives from academia,
industry, and government research institutions. The session will consist of a student-run question and answer session
followed by an interactive Q&A segment with the audience. So, come and learn all the answers to life’s big questions (or
at least get an idea of what you want to do in your career.)
Panelists:
Michael E. Kane, University of Florida
Mary Ann McGill, University of Wisconsin
Sylvia Adjoa Mitchell, University of the West Indies
Nancy A. Reichert, Mississippi State University
Elizabeth J. Roemer, State University of New York – Stony Brook