SUNDAY, JUNE 5 – THEME: MOLECULAR & CELLULAR
7:00 am – 7:00 pm
Registration
Atrium Foyer
FUNCTIONAL GENOMICS
AND PROTEOMICS
Conveners:
Linda B. Jacobsen, Roche Applied Science, and Mark C. Jordan, Agriculture &
Agri-Food Canada
8:00 am - 10:00 am
Plenary Symposium
Constellation A
The techniques of genomics and proteomics have recently become increasingly available to
academic scientists studying specific biological problems. Many academic and public sector
institutions have access to microarray, mass spectrometry and bioinformatics facilities. In
addition, the number of organisms for which off the shelf gene chips are available is rapidly
increasing. Quantities of proteins can be made with specific mutations to study structures and
molecular interactions. In this session we will look in depth at how gene expression analysis
and proteomics are being utilized in applied biology. The specific application of producing
recombinant proteins in plants for human health applications will be described.
8:00
Introduction (L. B. Jacobsen and M. C. Jordan)
8:10
PS-1
Interplay of Gene-specific Disease Resistance, Basal Defense, and the
Suppression of Host-responses
Roger Philip Wise, USDA-ARS
8:45
PS-2
New Markers for Human Tumors: A High-throughput Computational Approach
Anna Baranova, George Mason University
9:20
PS-3
Plant-made Pharmaceuticals: Where Are We Now?
Delia R. Bethell, Ventria Bioscience
PLANT TISSUE CULTURE
EDUCATION: KITS AVAILABLE FOR THE CLASSROOM
Convener:
Carol M. Stiff, Kitchen Culture Kits, Inc.
8:00 am – 10:00 am
Education Workshop
Baltimore
Educators have little time and less money to incorporate new technologies into their
curriculum. With the aid of commercial kits, teachers can more easily accomplish this goal and
do hands-on plant tissue culture experiments. We will give the teachers an overview of plant
tissue culture and show how the various kits could be used.
8:00
Introduction (C. M. Stiff)
8:10
W-5
The In Vitro Collecting Kit
Valerie C. Pence, Cincinnati Zoo and Botanical Gardens – Center for
Conservation and Research of Endangered Wildlife
8:35
W-6
Tissue Culture Kits from PhytoTechnology Labs
Gary Seckinger, PhytoTechnology Labs
9:00
W-7
Kits from Carolina Biological, Ward’s Scientific, and Kitchen Culture Kits
Carol M. Stiff, Kitchen Culture Kits
9:25
W-8
Jamaican “Do-it-yourself” Tissue Culture Kit
Sylvia Mitchell, University of the West Indies
10:00 am – 10:30 am
Coffee Break
Atrium Foyer
ELECTRICAL METHODS
FOR GENE DELIVERY
Conveners:
Linda B. Jacobsen, Roche Applied Science, and Richard Heller, University of
South Florida
10:30 am – 12:30 pm
Animal Symposium
Annapolis
Electrical currents can be used to produce small holes in the cell membrane. DNA molecules
can then pass through these holes. The DNA is then able to reach the nucleus and new
protein is synthesized. Speakers at this session will describe how this technology can be used
to deliver genes to the whole animal via the lung, and cells that are notoriously difficult to
transfect such as primary cells, be they stem cells or differentiated non-dividing stem cells.
10:30
Introduction (L. B. Jacobsen and R. Heller)
10:35
A-1
Electroporation is a Versatile Tool for Delivering Plasmid DNA
Richard Heller, University of South Florida
11:10
A-2
In Vivo Gene Delivery with Pulsed Electrical Fields
Robert W. Malone, Gene Delivery Alliance
11:50
A-3
Transfection of Neurons and Neural Stem Cells by Nucleofection
Eyleen L. K. Goh, Johns Hopkins University
EMBRYOGENESIS/REGENERATION/MICROPROPAGATION
Moderator:
Bipul K. Biswas, Fort Valley State University
10:30 am – 12:30 pm
Plant Contributed Paper Session
Frederick/Columbia
10:30
P-1000
Large Scale In Vitro Bulblet Production in Endemic and Endangered
Flowerbulbs Sternbergia candida and Muscari muscarimi
Sebahattin Özcan, Ankara University, Ýskender Parmaksýz, Semra Mirici,
Satý Çöçü, Cengiz Sancak, Serkan Uranbey, Ercüment O. Sarýhan, Bilal
Gürbüz, Cafer S. Sevimay, and Neþet Arslan
10:45
P-1001
Efficient Micropropagation Protocol of an Antimalarial Plant Spilanthes
acmella L. var. oleraceae
Vibha Pandey, University of Delhi, Kuldeep Sharma, and Veena Agrawal
11:00
P-1002
Plant Regeneration in Guava Through Somatic Embryogenesis
Bipul K. Biswas, Fort Valley State University, N. Joshee, A. Yadav, and A.
K. Yadav
11:15
P-1003
Comparison of Methods of Liquid Medium Culture and Effects of Temporary
Immersion of Explants for Banana (cv Dwarf Cavandish) Micropropagation
Farah Farahani, Islamic Azad University, Ahmad Majd, and Reza Zarghami
11:30
P-1004
Direct Shoot Bud Differentiation and Plantlet Regeneration from Leaf and
Petiole Explants of Begonia tuberhybrida
K. Meeker, University of Toledo, S. Nada, Siva Chennareddy, R. V. Sairam,
S. Zhang, T. V. Reddy, and S. L. Goldman
11:45
P-1005
Extensive Field Evaluation fo Staple Food Crop Varieties on Atolls: Collected
Around the Globe and Multiplied by Tissue Culture
Virendra Mohan Verma, College of the Marshall Islands, John J. Cho, Phillip
Jackson, Jabukja Aikne, and Jina David
12:00
P-1006
In Vitro Seed Germination and Seedling Development of Habenaria
macroceratitis, an Endangered Terrestrial Orchid
Scott L. Stewart, University of Florida, and M. E. Kane
12:15
P-1007
In Vitro Germination and Seedling Development of Calopogon tuberosus,
Philip J. Kauth, University of Florida, W. A. Vendrame, and M. E. Kane
12:30
P-1008
Efficient Organogenesis and Somatic Embryogenesis and Mean Metabolic
Heat and RCO2 Production Rates Measured by Microcalorimetry During
Different Micropropagational Induction Processes of Cineraria ‘Jester Pink’,
Gyeong Hee Kim, Gyeongsang National University, David W. Burger, Heiner
Lieth, and Byoung Ryong Jeong
IN VITRO STUDIES
OF CHRONIC VIRAL INFECTIONS ASSOCIATED WITH SIGNIFICANT
HUMAN CANCERS
Conveners:
Alda Vidrich, University of Virginia Health Sciences Center, and Craig Meyers,
Penn State College of Medicine
10:30 am – 12:30 pm
Animal Symposium
Baltimore
Despite decades of intensive research efforts, cancer is still a major human health problem.
Globally, over 5 million will die each year from cancer. Research has provided an
appreciation for the role of viruses in human carcinogenesis and has shown viruses to be
excellent model systems for the carcinogenic process in general. In vitro cell culture
techniques are a vital tool for the investigation of the role of viruses in the initiation and
progression of cancer. This Special Session In Depth is fortunate to have three outstanding
leaders in viral carcinogenesis whose work exemplifies the importance of in vitro culture
systems.
10:30
Introduction (A. Vidrich and C. Meyers)
10:40
A-4
Genetic Dissection of Human Papilllomavirus Oncogene Functions in
Organotypic Epithelial Raft Cultures
Thomas R. Broker, University of Alabama at Birmingham
11:25
A-5
Using the HBV Recombinant Baculovirus/HepG2 System to Understand HBV
Pathogenesis
Harriet C. Isom, Penn State College of Medicine
12:10
A-6
Methylthiophanate-induced Genotoxicity and Development of Single Strand
Breaks in DNA
Javed Musarrat, Aligarh Muslim University
METABOLIC ENGINEERING/SECONDARY
METABOLISM
Conveners:
Pamela J. Weathers, Worcester Polytechnic Institute, and Suman Bagga, New
Mexico State University
10:30 am – 12:30 pm
Plant Symposium
Chesapeake A&B
Plants produce a vast array of structurally and functionally diverse secondary metabolites. For
many years an empirical approach was the main method used to attempt the overproduction
of these chemicals in planta. Despite much effort there have been very few success stories.
More recently, a more systematic approach has been used whereby pathway fluxes have
been used to predict which genes should be stimulated or blocked in order to direct carbon
into a desired product. This metabolic engineering, albeit initially very tedious, has proved
successful and has yielded many other approaches to producing secondary metabolites as
well as other plant compounds in a controlled and predictable manner. This session provides
examples of very different approaches to engineering the production of a diversity of plant
products.
10:30
Introduction (P. J. Weathers and S. Bagga)
10:40
P-1
Engineering of N and S Metabolism
Champa Sengupta-Gopalan, New Mexico State University
11:15
P-2
Terpenoid Engineering from Plants to Bacteria
Sydnor Withers, University of California – Berkeley
11:50
P-3
Use of Metabolic Modeling for Metabolic Engineering
David Rhodes, Purdue University
RNAI: OVERVIEW AND
RECIPES FOR SUCCESS...HOW TO KICK IT DOWN A NOTCH
Conveners:
Janis L. Demetrulias, Technikos Research Associates, and Michael J. Fay,
Midwestern University
10:30 am – 12:30 pm
Animal Workshop
Constellation A
RNA interference (RNAi) describes the process in which double stranded RNA (dsRNA)
promotes the destruction of homologous mRNA in a cell. As is true with every powerful new
research tool, there are numerous commercial reagents for performing RNAi experiments.
The goal of this workshop is to provide the attendees with basic information about RNAi from
the viewpoint of a company providing RNAi reagents and from the viewpoint of a research
scientist utilizing this powerful new technology. Information gained from this workshop will
provide the attendee with basic information necessary for successfully knocking down the
expression of their favorite gene.
10:00
Introduction (J. L. Demetrulias and M. J. Fay)
10:35
W-9
Global RNAi Phenotype Analysis for Cancer Drug Target Identification
Spyro Mousses, Translational Genomics Research Institute
11:25
W-10
From Basics to Practice - Methodologies and Applications of Highly Effective
and Specific RNAi
Kerry E. Lowrie, Invitrogen Corporation
12:15
Open Discussion and Q & A
FROM BENCHTOP TO
APPLICATION: CLINICAL ROUTE
Convener:
John W. Harbell, Institute for In Vitro Sciences
1:30 pm – 3:00 pm
Animal Workshop
Baltimore/Annapolis
This session will explore the use of mammalian cells in the discovery and development of
biopharmaceutical products for therapeutic use as regulated products of drugs, biologics,
including biological devices and cellular therapies, or medical devices with biological
components. Attention will be focused on pragmatic solutions for the early screening and
advancement of candidates, recognition of significant regulatory considerations at the earliest
stages of development and practical considerations in the early-stage manufacture of
products for pre-clinical and clinical trial evaluation.
1:30
Introduction (J. W. Harbell)
1:40
W-11
Practical and Regulatory Considerations in the Development of Mammalian
Cell-based Biopharmaceuticals
W. Alan Moore, Althea Technologies
Conveners:
Richard Heller, University of South Florida, and Wallace L. McKeehan, Texas
A&M University Health Sciences Center
1:30 pm – 3:00 pm
Animal Workshop
Frederick/Columbia
The development of efficient gene transfer protocols impacts both basic research and the
potential for treating a wide variety of diseases. These protocols cover a variety of
applications and include delivery in the in vitro, ex vivo and in vivo settings. As the field of
gene therapy has progressed, the use of non-viral delivery methods has steadily increased.
This session will explore recent advances in the use of gene transfer and will focus on non-
viral delivery protocols.
1:30
Introduction (R. Heller and W. L. McKeehan)
1:40
W-12
Nucleofection
®
: A Novel Non-viral Transfection Method for Primary Cells and
Hard-to-transfect Cell Lines
Titus Kretzschmar, amaxa GmbH
2:20
W-13
Considerations When Using Lipid Based Transfection Reagents
Linda B. Jacobsen, Roche Applied Science
Conveners:
Melissa E. Hinga, RiceTec, Inc., and Ebrahim Firoozabady, Del Monte Fresh
Produce Company
1:30 pm – 3:00 pm
Plant Symposium
Constellation A
Control of gene expression has gone from a place on the plant geneticists “wish list” of tools to
a “pick and play” solution to regulation of such complex traits as temperature sensitivity, cell
cycle control and plant morphology. Public and private databases allow one to identify genetic
sequences having the typical components of a transcription factor. A database of transcription
factors divided into families has been identified from the fully sequenced indica rice genome.
Beyond the public research initiatives into understanding and applying transcription factors,
private companies have formed which are offering defined target transcription factors and
methods to identify factors with specific regulatory function. Our invited speakers will first
provide us with a broad picture of the status of the science and a look at one private company
involved in transcription factor discovery and then a look at applied research in the use of
transcription factors to modulate gene expression.
1:30
Introduction (M. E. Hinga and E. Firoozabady)
1:35
P-4
Plant Transcription Factors: Gene Families, Family Evolution, Functions with
Ectopic Expression, and Applications
Neal Gutterson, Mendel Biotechnology, Inc.
2:15
P-5
Gene Regulons and Regulatory Networks Involved in Plant Cold Acclimation
Mike Thomashow, Michigan State University
2:40
P-6
Increased Cuticular Wax Accumulation and Enhanced Drought Tolerance in
Transgenic Alfalfa by Overexpression of a Transcription Factor Gene
Zeng-Yu Wang, The Samuel Roberts Noble Foundation
Convener:
Lining Tian, Agriculture and Agri-Food Canada
1:30 pm – 3:30 pm
Plant Symposium
Chesapeake A&B
Tree improvement for agriculture, forestry and ecological conservation, by traditional methods,
has been a slow process limited by slow growth and long life cycles. Past years have seen
successful implementation of plant biotechnology into these areas. This session will update
the progress of tree biotechnology and will show that it is a valuable and effective tool for tree
improvement and utilization.
1:30
Introduction (L. Tian)
1:35
P-7
Developing a Commercial Somatic Embryogenesis Platform for Large Scale
Production of Conifer Seedlings
Stephen Attree, CellFor, Inc.
2:05
P-8
Plum Pox Virus Resistant Plums - A Case Study of the Potentials and Pitfalls of
Genetically Engineered Tree Fruits
Ralph Scorza, USDA/ARS
2:35
P-9
Can GE Trees Help the Environment?
Scott Merkle, University of Georgia
2005 IN VITRO BIOLOGY
OPENING CEREMONY
Program Chair: Gordana V. Vunjak-Novakovic, Massachusetts Institute of Technology
3:30 pm – 5:00 pm
Opening Ceremony
Constellation A
Conveners:
David W. Altman, IPR Consulting, and Gordana V. Vunjak-Novakovic,
Massachusetts Institute of Technology
3:30
Welcome and Opening Remarks
David W. Altman, President, Society for In Vitro Biology
3:40
2005 Student Award Winner Presentations
3:50
2005 Lifetime Achievement Award Recipient
4:00
Keynote Symposium
Advances in Tissue Engineering
Introduction (G. V. Vunjak-Novakovic)
KS-1
Robert Langer, Professor of Chemical and Biomedical Engineering at the
Massachusetts Institute of Technology
CHARACTERIZATION
OF ANIMAL CELLS
Conveners:
John Masters, University College of London, and Yvonne A. Reid, ATCC
5:00 pm – 6:30 pm
Animal Symposium
Frederick/Columbia
Are you certain that the cells that you are culturing are what you think they are? Many
scientists just accept that whatever is written on the flask is correct and do not check the
authenticity of the cells that they have been given or acquired. In this symposium, expert
scentists from the American Type Culture Collection (ATCC) will describe methods that can
be used in every laboratory to check the provenance and characteristics of cell cultures, as
well as some more specialized techniques for defining specific features of cells and other
characteristics that can change with time and culture, such as gene expression.
5:00
Introduction (J. Masters and Y. A. Reid)
5:10
A-7
Authentication and Characterization Procedures of Animal Cell Lines at ATCC
Yvonne A. Reid, ATCC
5:30
A-8
Signs of Pluripotency: Characterization of Embryonic Stem Cell Lines
Richard Josephson, ATCC
5:50
A-9
DNA Profiling and the Authentication of Cell Lines
John R. W. Masters, University College of London
6:10
A-10
High Passage Number of Continuous Cell Line Affects the Transcriptome
Pranvera Ikonomi, ATCC
Conveners:
Peggy J. Ozias-Akins, University of Georgia, Dennis A. Laska, Eli Lilly and
Company, and Alvar Carlson, University of Wisconsin – Madison
5:00 pm – 6:00 pm
Plant Workshop
Chesapeake A&B
Fluorescent markers have numerous applications in cellular and molecular biology. Nucleic
acids, proteins, and lipids, among other macromolecules, can be labeled or fused, or interact
with fluorescent molecules to enable identification, localization or separation of cells or cellular
components. Fluorescent markers, such as green fluorescent protein (GFP), the native
protein of which was discovered in the jellyfish, Aequorea victoria, has had considerable
application in plant transformation as a non-destructive reporter and fusion protein. Both
natural and induced fluorescent protein diversity have been exploited for multiplexed
approaches to analyzing cellular structure and function. This workshop will focus on the
detection and application of fluorescent markers in plant and animal cells.
5:00
Introduction (P. J. Ozias-Akins, D. A. Laska, and A. Carlson)
5:10
W-14
Higher Content Fluorescent Assays
Jurgen Vanhauwe, Invitrogen Corp.
5:35
W-15
Fluorescent Protein Utility in Transgenic Plants
C. Neal Stewart, Jr., University of Tennessee
IN VITRO TOOLS, TECHNIQUES,
AND OPTIMIZATION
Moderator:
Jeffrey Adelberg, Clemson University
5:00 pm – 6:00 pm
Plant Contributed Paper Session
Constellation A
5:00
P-1009
Growth and Sugar Use in Agar and Liquid with Large and Small Vessels
Using Tumeric (Curcuma longa) as a Model Crop
Jeffrey Adelberg, Clemson University
5:15
P-1011
Movement and Containment of Microbial Contamination in the Nutrient Mist
Bioreactor
Mahmoud A. Sharaf-Eldin, Worcester Polytechnic Institute, and P. J.
Weathers
5:30
P-1012
Physical Interactions Between Aminoglycoside Antibiotics and Gelrite:
Documentation and Effects on Antibiotic Efficacy
Michael J. Bosela, Indiana University – Purdue University at Fort Wayne
5:45
P-1013
Optimum Ventilation in Culture Vessels Reduces the Hyperhydricity in
Shoot Cultures of Scrophularia yoshimurae
Hsin-Sheng Tsay, Chaoyang University of Technology, Satish Manohar
Nalawade, Wei Fang, and Chien-Chou Lai
7:00 pm – 9:00 pm
2005 IN VITRO BIOLOGY MEETING
Constellation B
OPENING RECEPTION
7:00 pm – 9:00 pm
CHARM CITY SILENT AUCTION KICKOFF
Constellation B
7:00 pm – 9:00 pm
Exhibits and Posters
Constellation B
Sunday, June 5
All Poster Authors will be present
7:30 pm – 8:30 pm