WEDNESDAY, MAY 26
7:00 am – 5:00 pm
Registration..................Market Street Foyer
CELL MODELS/BIOTECHNOLOGY
Moderator:
Lynne Rutzky, The University of Texas Medical
School
8:00 am – 10:00 am
Joint Vertebrate/Toxicology Contributed Paper Session...........Seacliff C & D
8:15
VT-1007
Adult Mammalian Spinal Cord Neuron Culture in a Defined System
Mainak Das, Clemson University, Cassie Gregory, Neelima Bhargava, Peter Molnar, Lisa Riedel, and
James J. Hickman
8:30
VT-1008
A Defined System for the Systematic Study of Cardiac Myocyte Development and Physiology –
Applications for Biocompatibility and Toxin Detection
Peter Molnar, Anupama Natarajan, Anahita Jamshidi, Mainak Das, Cassie Gregory, ChangJu
Chung, and James J. Hickman
8:45
VT-1009
The Multisolution Hypothesis of Cell Preservation
Robert G. Van Buskirk, Binghamton University, Aby J. Mathew, John M. Baust, Kristi Karin Snyder,
and John G. Baust
9:00
VT-1010
Hypothermic Storage of Neonatal Mammalian Cardiomyocytes: Assessment of Multiple Markers of
Viability
Kristi Karin Snyder, Binghamton University, John M. Baust, Robert G. Van Buskirk, and John G.
Baust
9:15
VT-1011
Modeled Microgravity Culture of Human Pancreatic Islets Improves Function and Reduces Apoptosis
Lynne P. Rutzky¸The University of Texas Medical School, Szczepan Bilinski, Malgorzata Kloc,
Huimin Zhang, Tammy Phan, Stephen M. Katz, and Stanislaw Stepkowski
GENE DISCOVERY AND
REGULATION
Moderator:
Lining Tian, Agriculture and Agri-Food Canada
8:00 am – 10:00 am
Plant Contributed Paper Session......Grand Ballroom C
8:00
P-1008
Modern Techniques of Genetic Engineering: Agrobacterium-mediated Transformation of Creeping
Bentgrass cv. PennA4 Using a Mutant BAS1 Construct for the Production of Dwarf Plants
J. Powell, University of Rhode Island, Chip Longo, Mike Budziszek, Alissa Neill, Joel Hague, Marina
Ayrapetov, Joel Chandlee, Amy Gaudette, Brian Steere, Rachel Maxwell, Cathy Buckley, C. Smith,
Jeremy Lins, Nicole Crothers, Alison Clark, Kristin Masso, Albert P. Kausch, and Michael Neff
8:15
P-1009
Improving Forage Quality of Tall Fescue (Festuca Arundiacea) by Genetic Manipulation of Lignin
Biosynthesis
Zeng-Yu Wang, The Samuel Roberts Noble Foundation, Lei Chen, Chung-Kyoon Auh, Paul Dowling
Andrew Hopkins, and Jeremey Bell
8:30
P-1010
Transformation of Solanum phureja to Enhance Tuber Carotenoid Levels
Steve Millam, Scottish Crop Research Institute, L. Ducreux, W. Morris, and M. Taylor
8:45
P-1011
Developing a Nicotiana benthamiana Network to Study Functional Genomics and Medicinal Plant
Biology
Monto H. Kumagai, University of Hawaii
9:00
P-1012
Repression of Gene Expression by HD2-type Histone Deacetylases and Function Analysis of HD2
Proteins in Arabidopsis
Lining Tian, Agriculture and Agri-Food Canada, Keqiang Wu, Changhe Zhou, Helene Labbe,
Sunandini Sridha, Li Wang, Marysia Latoszek-Green, Lin Zhang, Daniel C. Brown, and Brian L. Miki
9:15
P-1013
A Barley LTP Promoter for Tissue-specific Expression of Transgene-mediated Disease Resistance
Maria L. Federico, University of Wisconsin-Madison, R. W. Skadsen, and H. F. Kaeppler
9:30
P-1014
Ac/Ds Transposon-mediated Gene Discovery in Barley
Jaswinder Singh, University of California - Berkeley, Shibo Zhang, L. Cooper, S. Rangel, R. Kaur, A.
K. Sturbaum, V. Edwards, Phil Bregitzer, P. M. Hayes, and Peggy G. Lemaux
9:45
P-1015
Differential Gene Expression During Embryo Development in Maritime Pine (Pinus pinaster)
Sonia C. M. Goncalves, J. Cairney, M. Oliveira, and C. Miguel
MEDICINAL PLANT
BIOTECHNOLOGY
Convener:
Anand K. Yadav, Fort Valley State University
Marceline Egnin, Tuskegee University
8:00 am – 10:00 am
Plant Symposium.................
Grand Ballroom B
The booming popularity of plant remedies actually began when the US Congress passed the Dietary Supplement Health
and Education Act in 1994. World trade in different types of medicinal plant products has been steadily increasing owing
to changing perceptions of prescription drugs and public preference for the botanical remedies without having side effects.
The primary objective of this workshop is to assess the knowledge of current trends in biotechnology of medicinal and
nutraceutical plants. Thus, this workshop provides an opportunity to exchange information on the current status of on-
going biotechnology related research efforts on medicinal and nutraceutical plants. Experts working on different
medicinal/nutraceutical plant species will emphasize their current specialty research work.
8:00
Introduction
8:10
P-30
Environmental and Genetic Approaches to Increase Productivity of Medicinal Plants in Liquid Culture
Jeffrey Adelberg, Clemson University
8:30
P-31
In Vitro Regeneration and Greenhouse Establishment of Medicinal and Rare Skullcaps (Scutellaria)
Nirmal Joshee, Fort Valley State University
8:50
P-32
Tissue Culture Methods to Improve Herbs, Spices, and Nutraceutical Plants
Alison M. R. Ferrie, Plant Biotechnology Institute/National Research Council Canada
9:10
P-33
Developing Biotechnology to Regenerate and Improve Selected Nutraceutical Plants
Bipul K. Biswas, Fort Valley State University
9:30
P-34
Prospects for Engineering Vaccinium Germplasm for the Production of a High-potency Resveratrol
Analogue
Daniel C. Cook, USDA/ARS/NPURU
VALIDATION AND REGULATORY
ACCEPTANCE OF IN VITRO TOXICITY ASSAYS
Convener:
John W. Harbell, Institute for In Vitro Sciences
8:00 am – 10:00 am
Animal Symposium
......................Seacliff A & B
For the past four to five decades, the Tissue Culture Associations, worldwide, have sought to promote cell-based research
and set standards for cell models, study design and publications. These efforts have succeeded to the point where cell
culture methods have evolved from strictly academic tools to mainstays of many fields including biotechnology (plant and
animal), drug discovery, and, increasingly, toxicology. This wider application often falls into the general category of
bioassays where the cell culture systems are used prospectively to predict an action on the whole organ or organism. For
many reasons, these in vitro methods are being applied to regulatory questions as well (e.g., drug efficacy, drug safety,
environmental toxicology). Any bioassay is designed around four components; a specific cell or tissue, defined exposure
conditions, well characterized endpoint measures of the biological effect, and a predetermined relationship between the in
vitro endpoint values and the effect being predicted (e.g., potency, organ toxicity, etc.). The predictive capacity of the
assay is dependent on maintaining the integrity and consistency of the whole system over time and often across
laboratories. This symposium will examine the “good science” aspects of cell/tissue culture studies and how they
contribute to the quality of the data generated. The topics will include Good Cell Culture Practices, study design
optimization, and understanding the predictive capacity of tests. Special emphasis will be given to the design and
validation of bioassays intended for use in the regulated environment.
8:00
Introduction (J. W. Harbell)
8:15
A-29
Good Cell Culture Practices Guidelines
Sandra Coecke, European Centre for the Validation of Alternative Methods
8:45
A-30
In Vitro Bioassays in Toxicology: Focus on Design, Validation, and Regulatory Acceptance
John W. Harbell, Institute for In Vitro Sciences
9:15
A-31
Development and Application of In Vitro Assays to Predict Human Response
Eugene Elmore, University of California – Irvine
10:00 am – 10:30 am
Coffee Break
.................Market Street Foyer
MICROSCOPY FOR THE
21ST CENTURY: NEW WAYS TO “SEE” INSIDE THE CELL
Convener:
Alda Vidrich, University of Virginia Health Sciences Center
10:30 am – 12:30 pm
State-of-the-Art Symposium.....................Bayview A
Advances in our understanding of cellular biology and cellular architecture have been achieved, in large part, because of
innovative engineering in microscopic technology. Today, more than ever, the microscope coupled with laser technology
has become a powerful tool in the study of molecular processes and pathways at the single cell level. Proteomic and
genomic techniques are dependent on obtaining homogeneous cell populations. Laser Capture Microdissection enables
the directed isolation of pure populations of cells from heterogeneous samples. This technology allows cells, cell
aggregates, or discrete morphological structures to be selected and captured from tissue sections. These captured cells
are suitable for nucleic acid studies such as SNP analysis, endpoint and real-time RT-PCR and mRNA expression
profiling. Differential protein-expression profiling by SDS-PAGE and 2D-PAGE as well as protein identification by mass
spectrometric sequencing, peptide mass fingerprinting, in-gel zymography and Western blotting also can be carried out
with laser microdissected cells. This SID and workshop will illustrate the range of downstream applications in pathology,
cancer research, life sciences, medical diagnostics and biotechnology for Laser Microdissection Technology as well as
offer a practical view of new advances in instrumentation and sample handling.
10:30
Introduction (A. Vidrich)
10:45
SA-10
Laser Capture Microdissection and Analysis of Gene Expression of Airway Mucous and Serous
Gland Cells
Walter Finkbeiner, University of California – San Francisco
11:15
SA-11
Profiling of Microdissected Gastric Epithelial Cells Reveals a Cell Type Specific Response to
Helicobacter pylori Infection
Anne Mueller, Stanford University
11:45
SA-12
Use of Laser Capture Microdissection to Isolate Plant Cells
Lewis Feldman, University of California - Berkeley
Convener:
Alda Vidrich, University of Virginia Health Sciences Center
12:30 pm – 2:30 pm
State-of-the-Art Workshop......................Bayview A
12:30
Introduction (A. Vidrich)
12:45
W-4
AS LMD New Advances in Laser Microdissection Technology
Andy Lee, Leica Microsystems
1:15
W-5
Sample Preparation and Molecular Analysis for Laser Microdissection Samples
Janice Zhou
1:45
Hands-on Demo
THE FUTURE OF IMPROVED
NUTRITION THROUGH
MODERN BIOTECHNOLOGY
Convener:
Raymond D. Shillito, Bayer CropScience
2:30 pm – 4:30 pm
Plant Symposium..................Grand Ballroom B
Ensuring an ample and healthy food supply is needed for a number of reasons: a growing world population, rising
consumer demand for improved food quality and safety, and an increasing interest in nutritional enhancements to foods.
Traditional plant breeding has been successful in at least partially meeting these goals, and modern agricultural
biotechnology is proving to be a powerful complement to traditional methods. This session will give an overview of the
state of affairs regarding improvement of crops for improved nutrition, both by agricultural biotechnology and traditional
means. It will cover the essentials of the process by which they are evaluated for their potential impact on human/animal
nutrition and health, and describe new efforts to apply both traditional and modern methods for nutritional improvement of
crops for the developing world.
2:30
Introduction (R. Shillito)
2:45
P-35
Improved Nutrition Through Modern Biotechnology
Martina Newell-McGloughlin, University of California at Davis
3:15
P-36
Assessment Process for Nutritionally Enhanced Food and Feed Crops
Bruce M. Chassy, University of Illinois at Urbana-Champaign
3:45
P-37
HarvestPlus: Biofortification as a Feasible Means of Increasing the Availability of Nutrients to
Malnourished Populations
Ray Glahn, Cornell University
PLANT REGENERATION
AND MICROPROPAGATION
Moderator:
Susan J. Murch, National Tropical Botanical Garden
2:30 am – 4:45 am
Plant Contributed Paper Session......Grand Ballroom C
2:30
P-1016
Photoautotrophic Medium and PPMTM Help to Alleviate Losses from Bacterial Contamination in
Papaya Micropropagation
Maureen M. M. Fitch, USDA/ARS, T. Leong, N. Saito, G. Yamamoto, A. Dela Cruz, A. Yeh, S. White,
S. Maeda, and P. Moore
2:45
P-1017
In Vitro Conservation of Endangered Plants in Hawaii
Susan J. Murch, National Tropical Botanical Garden
3:00
P-1018
Plant Regeneration of Taro (Colocasia esculenta (L.) Schott) via Somatic Embryogenesis and
Organogenesis
Virendra Mohan Verma, College of the Marshall Islands, and John J. Cho
3:15
P-1019
In Vitro Micropropagation of Ginger (Zingiber officinale Rosc.) and Turmeric (Curcuma longa L.)
Xiaoqiang Ma, University of Arizona, and David R. Gang
3:30
P-1020
Qualitative and Quantitative Monitoring of Gene Expression Patterns Among Micropropagated Plants
of Tea Clones by Differential Screening of Randomly Amplified cDNAs Using RAPD Primers
Soom Nath Raina, University of Delhi, and S. Goel
3:45
P-1021
Micropropagation Saves Threatened Citrus Germplasm in Kosrae
Puthiyaparambil Chacko Josekutty, College of Micronesia – FSM, E. B. Langu, S. S. Cornelius, T.
N. Kilafwasru, and N. S. Nena
4:00
P-1022
Somatic Embryogenic Response of Petiveria alliacea Leaf Explant-derived Callus to Sucrose and
Glucose Enriched Media Supplemented with NAA and BAP
Seymour Augustus Webster, University of the West Indies, S. A. Mitchell, and M. H. Ahmad
4:15
P-1023
Conservation of Endangered Species Through Plant Tissue Culture in Mexican Botanical Gardens
V. Chávez, Jardin Botanico Francisco Javier Clavijero, Victor Manuel Chávez-Avila, A. Martínez, R.
Baltazar, E. Pérez, V. Salazar, P. Ortega, and R. Litz
4:30
P-1024
Eugenol Content of In Vitro and In Vivo Ocimum Species – A Comparative Gas Chromatographic
Analyses
Sivaramakrishnan Sudhakaran, Asian Institute of Medicine, Science and Technology, and V.
Sivasankari