SUNDAY, MAY 23
7:00 am – 5:00 pm
Registration..................Market Street Foyer
ADVANCES IN AUTOMATION
OF CELL CULTURE AND CELL-BASED ASSAYS
Conveners:
Dennis A. Laska, Eli Lilly and Company
Linda B. Jacobsen, Roche Diagnostics Corporation
8:00 am – 10:00 am
Animal Symposium
......................Seacliff A & B
The growth in application of cell-based assays by academic and industrial life science laboratories has led to
automation advances in both cell culture maintenance and assay conduct. Automation of repetitive activities
required to routinely perform cell-based assays has helped reduce the labor intensity and open the bottleneck
associated with these assays. This session will introduce the participants to several novel advances in automation
technology, as well as provide examples where implementation of partial or total assay automation has led to
increased throughput, tighter data, and reduced costs. Additionally, most automation equipment is designed in
modular form and can be tailored to fit specific current needs and expanded in the future as need increases, thus
these opportunities are not limited to large core-laboratory application or to industrial settings.
8:00
Introduction (D. Laska)
8:05
A-1
Cost Benefit of Small Automation Systems in Cell-based Laboratories
Joseph Bertoncini, Brandel Corporation
8:30
A-2
A Microcapillary Cytometry System for Cell-based Screening Assays
Keith Olson, Guava Technologies
8:55
A-3
Automation of Cellular Analysis and Gene Expression
Laura Pajak, Beckman Coulter, Inc.
9:20
A-4
Applications of Automated Cell Culture Technologies to Enhance Assay Throughput
Linda B. Jacobsen, Roche Diagnostics Corporation
FUNCTIONAL GENOMICS
OF AQUATIC TOXICOLOGY
Convener:
J. Denry Sato, Mount Desert Island Biological Laboratory
8:00 am – 10:00 am
Invertebrate Symposium
..................Seacliff C & D
Fish represent a diverse group of vertebrates living in a wide variety of environments that vary with respect to
oxygen tension, salinity, temperature, pressure, light intensity, and composition of natural and manmade chemical
toxicants. In recent years, comparative studies of fish and mammalian genomes have shown that these taxonomic
groups, which diverged over 400 million years ago, share much in the way of conservation of genome organization
and gene function. Thus, it is likely that functional genomic studies of evolutionary and physiological adaptations
that allow fish to live in diverse environments will also provide insight into normal and pathological human
physiology. The talks in this session will present new tools and organisms for studying the impact of aquatic
environmental toxicants on physiological processes.
8:00
Introduction (J. D. Sato)
8:05
I-13
Comparative Approaches to Understanding Mechanisms of Toxicity: The Comparative
Toxicogenomics Database (CTD)
Carolyn Mattingly, Mount Desert Island Biological Laboratory
8:45
I-14
“Fish & Chips” Using DNA Arrays to Study Environmental Stress in Non-model Organisms
Andrew Gracey, Stanford University
9:25
I-15
The Effects of Arsenic on the Function of CFTR Cl Channels in Killifish, a Euryhaline Teleost
Bruce A. Stanton, Dartmouth Medical School
PLANT PATHOGEN GENOMICS
AND TRANSFORMATION
Convener:
Yinghui Dan, Virginia Tech
8:00 am – 10:00 am
Plant Symposium..................Grand Ballroom C
Plant pathogens cause significant economic damage to the agricultural production worldwide by direct reduction of yields
and losses of market and export. How do plant pathogens infect host plants and establish disease interactions with hot
plants? How do they reproduce and spread in the plants, and subsequently persist and become disseminated in the
environment? How can we control them? The sciences of genomics and transformation are leading to an advanced
understanding of how genes function in a coordinated fashion that results in various organismal characteristics or
phenotypes. Functional, structural, and comparative genome analysis of plant pathogens will provide insights to
understand the questions. These understandings are essential to the development of effective and environmentally sound
strategies for disease control. This symposium will provide an update on a genomics initiative and transformation of plant
pathogens that have a significant agricultural impact.
8:00
Introduction (Y. Dan)
8:15
P-1
Using the Genomic Sequence to Study Development and Pathogenicity of the Head Blight Fungus
Frances Trail, Michigan State University
8:45
P-2
Fungal Transformation: A Key Tool for Studying the Molecular and Cellular Basis of Fungal Diseases
Seogchan Kang, The Pennsylvania State University
9:15
P-3
Agrobacterium tumefaciens: Gene Transfer from Prokaryotes to Eukaryotes
Eugene Nester, University of Washington, Seattle
THINKING OUTSIDE
THE CELL
Convener:
Praveen Saxena, University of Guelph
8:00 am – 10:00 am
Plant Symposium..................Grand Ballroom B
Protoplasts are naked plant cells, stripped of the cell wall and wrapped only with a plasma membrane. Numerous aspects
of the biology of plant structure, function, and development including cell cycle, cell differentiation, and oxidative stress
can all be investigated with protoplast model systems. Isolated protoplasts also provide an excellent opportunity for the
study of transfer, integration, and expression of foreign DNA as genes, chromosomes, and even entire organelles,
combining multiple genes across sexual, taxonomic, and other incompatibility barriers. Somatic fusion, the process of
combining individual cells of different species, has tremendous potential in development of rare and completely new
hybrids. This approach is uniquely suited to medicinal species for which new plant types can be created with specific
combinations of nutritional and medicinal metabolites that represents a novel approach for the improvement of human
health. This symposium is designed to review the progress made in protoplast technologies, to stimulate a renewed
interest in this research, and to find new applications of these technologies for crop improvement.
8:00
Introduction (P. Saxena)
8:15
P-4
Plant Protoplasts: Current Status of Culture Technologies to Underpin Somatic Hybridization and
Transformation
Michael R. Davey, University of Nottingham
8:45
P-5
Applications of Somatic Hybridization and Cybridization in Crop Improvement, with Citrus as a Model
Jude Grosser, University of Florida
9:15
P-6
Protoplast Technology and Medicinal Plants
Praveen Saxena, University of Guelph
10:00 am – 10:30 am
Coffee Break
....................Pacific Concourse
10:00 am – 3:00 pm
Exhibits and Posters
..............Pacific Concourse
2004 WORLD CONGRESS
OPENING CEREMONY
WORLD CONGRESS SPONSORS:
SOCIETY FOR IN VITRO BIOLOGY
CANADIAN CHAPTER OF THE IAPTC&B
EUROPEAN TISSUE CULTURE SOCIETY
JAPANESE ASSOCIATION FOR ANIMAL CELL TECHNOLOGY
JAPANESE SOCIETY OF PLANT CELL AND MOLECULAR BIOLOGY
SWISS TISSUE CULTURE SOCIETY
THE JAPANESE TISSUE CULTURE ASSOCIATION
Program Chair:
Wayne A. Parrott, University of Georgia Crop & Soil Sciences
Program Co-chair:
Gordana V. Vunjak-Novakovic, Massachusetts Institute of Technology
10:30 am – 12:30 pm
Opening Ceremony................Grand Ballroom C
Conveners:
Sandra L. Schneider, Research & Clinical Laboratory Systems
Nam-ho Huh, Okayama University
Sanetaka Shirahata, Kyushu University
10:30
Welcome and Opening Remarks:
Sandra L. Schneider, President, Society for In Vitro Biology
10:40
2004 Lifetime Achievement Award Recipients:
Walter Nelson-Rees (See program for Award Presentation)
Thomas Grace (See program for Award Presentation)
Trevor A. Thorpe (See program for Award Presentation)
11:00
Distinguished Keynote Symposium
Chemically Programmable Immunity: The Challenge and Approach of Today’s Disease/Pathogen Drug
Treatments
Introduction (S. Schneider)
KS-1
Kary B. Mullis, Founder, Altermune, LLC, Newport Beach, California, and 1993 Nobel
Laureate in Chemistry “For the Invention of Polymerase Chain Reaction (PCR)”
12:30 pm – 1:30 pm
Distinguished Keynote Reception/Lunch in Exhibit Hall.....Pacific Concourse
1:30 pm – 2:30 pm
Even Poster Authors will be present
...............................Pacific Concourse
CELL AND CELL CULTURE
MODELS FOR TOXICOLOGY TESTING AND STUDIES/IN VITRO
TOOLS
Moderator:
Penny A. Jones, Unilever Colworth
1:30 pm – 2:15 pm
Vertebrate/Toxicology Interactive Poster Session..............Pacific Concourse
VT-2006
Reconstituted Human Epidermis: An Alternative Model to Assess Drug Metabolism, UV Irradiation
Effects and Efficacy of Antioxidant or Photoprotection
Alain Mavon, Pierre Fabre Research Institute, C. Dorio, C. Gelis, and V. Raufast
VT-2007
The Contribution of Calpains in the Downregulation of MDM2 and p53 Proteolysis in Reconstructed
Human Epidermis in Response to Solar Irradiation
C. Gelis, Université Paul Sabatier, A. Mavon, and P. Vicendo
VT-2008
Enterocytes Like Caco-2 Cells as a Model to Study the Interaction of Surfactant Proteins A and D and
Intestine
Bhanu Pratap Singh, University of London, Henno G. C. J. M. Hendriks, Henk P. Haagsman, and
Jaap E. Van Dijk
VT-2009
Capacity of Fish Cell Lines to Undergo Apoptosis in Response to Two Toxicants: Gliotoxin and
Copper
S. J. Dewitte-Orr, University of Waterloo, G. M. Weir, V. R. Dayeh, M. P. Lamb, and N. C. Bols
VT-2010
Relative Hazard Assessment In Vitro Using Commercially Available Human Epithelial Tissue Models
Penny A. Jones, Unilever Colworth, and A. V. Furniss
VT-2011
Evaluation of Lagerstroemia speciosa (Banaba) Extracts Using Cultured Human Adipocytes
Peter X. Zhang, Shaklee Corporation, M. Yatcilla, R. Ubillas, T. Ianiro, K. Taylor, and J. Hegenauer
CRYOPRESERVATION
/ IN VITRO TOOLS
Moderator:
Lia Campbell, Organ Recovery Systems
1:30 pm – 2:15 pm
Vertebrate/Toxicology Interactive Poster Session..............Pacific Concourse
VT-2000
Novel Cyropreservation Method for Primate Embryonic Stem Cell Lines
Isao Asaka, Asahi Techno Glass Corporation, H. Suemori, R. Okamoto, H. Sugai, Y. Kondo, Y.
Suzuki, S. Nito, T. Shimo-Oka, and N. Nakatsuji
VT-2001
Effect of Temperature and Length of Cold Storage on Artemia salina Flow-through Developmentally
Synchronous Culture
Linda M. Niedziela, Elon University, and N. Wright
VT-2002
Long Term Exposure to Disaccharides Produces Viable Cells After Preservation
Lia H. Campbell, Organ Recovery Systems, K. A. Sarver, K. R. Hylton, L. Sycuro, B. Leman, and K.
G. M. Brockbank
VT-2003
Use of a Robotic Cell Maintenance System to Produce a Cell Line Bank for the Avon Longitudinal
Study of Parents and Children (ALSPAC)
Patricia Holley, University of Bristol, Susan Ring, The ALSPAC Study Team, and Richard Jones
VT-2004
Glutamine Stability in Cell Culture Media
Alicia Kaye Elwood, HyClone Laboratories, Inc., B. Fujimoto, and B. Barnett
VT-2005
Effects of Carbon Dioxide-Sodium Bicarbonate Dynamic on CHO Cell Performance in a Protein-free,
Chemically Defined Medium
Paula N. Decaria, HyClone, Inc., J. Camire, and B. Barnett
Moderator:
Guy Smagghe, Ghent University
1:30 pm – 3:00 pm
Invertebrate Contributed Paper Session
...................................Seacliff C & D
1:30
I-1000
A New Method for the Rapid and Automated Generation of Baculovirus Expression Vectors
Kevin Stephen Richards, Oxford Brookes University, R. B. Hitchman, C. P. Nixon, C. H. Irving, S. G.
Mann, L. K. King, and R. D. Possee
1:45
I-1001
Response of Midgut Stem Cells of the Caterpillar Spodoptera littoralis to Insect Peptide Factors,
-
Arylphorine and Hormones
Guy Smagghe, Ghent University, S. Goto, D. De Wilde, W. Vanhassel, C. Moeremans, M. J. Loeb,
M. Blackburn, and R. S. Hakim
2:00
I-1002
Use of Gal4-Mos1 Cimeric mariner Transposase for Germ-line Transformation in Drosophila
melanogaster and Aedes aegypti
K. J. Maragatha Vally, Texas A&M University, Craig John Coates
2:15
I-1003
Changing the Fate of Stem Cells from the Midgut of the Insect, Heliothis virescens: Changing
Calcium Ion Concentration
Marcia J. Loeb, USDA
2:30
I-1004
Screening Invertebrate Cell Lines for Gap Junction Channel Activity and Functional Innexin Genes
Guido F. Caputo, Canadian Forest Service, S. J. Siddiqi, J. Chen, and J. Phipps
Moderator:
Allan Wenck, BASF Plant Science
2:00 pm – 3:00 pm
Plant Interactive Poster Session
...............................Pacific Concourse
P-2000
Protoplast Fusion for Transferring Late Blight and Colorado Potato Beetle Resistance Genes From
Mexican Wild Solanum Species into Cultivated Potato
Qin Chen, Agriculture and Agri-Food Canada, Y. Z. Shi, H. Y. Li, D. Beasley, D. Lynch, M. Goettel,
and L. Kawchuk
P-2001
Development and Evaluation of Transgenic Peanuts for Induced Resistance to the Indian Peanut
Clump Virus
Kiran K. Sharma, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), F.
Waliyar, M. Lavanya, and A. S. Reddy
P-2002
Characterization of Cultivated Peanut (Arachis hypogaea L.) Transformed with the N-gene of Tomato
Spotted Wilt Virus
Ye Chu, University of Georgia, H. Y. Yang, and P. Ozias-Akins
P-2003
Production of Disease Resistant Transgenic Pigeonpea
Prathibha C. Devi, Osmania University, K. K. Sharma, S. Manoj Kumar, and Suneetha Dayal
P-2004
Transgenic Tomato Expressing an Arabidopsis Thionin (Thi2.1) Countervails Phytopathogenic Attack
and Bears Palatable Fruits
Venkatesh Prasad, Academia Sinica, Li-Jen Liao, Chiu-Ping Cheng, Yuan-Li Chan, Shiang-Chun
Liu, Tsai-Hung Hsieh, and Ming-Tsair Chan
CELLULAR MODEL CHARACTERIZATION
Moderator:
Dianne Hammond, Wyle Life Sciences
2:15 pm – 3:00 pm
Vertebrate/Toxicology Interactive Poster Session..............Pacific Concourse
VT-2015
Establishment and Characterization of Cell Lines From 3 Human Thyroid Carcinomas: Responses to
All-trans-retinoic Acid and Mutations in the BRAF Gene
Ja-Lok Ku, Seoul National University College of Medicine, C.-S. Koh, S.-Y. Park, J.-H. Park, I.-J.
Kim, H. C. Kang, Y.-K. Shin, S.-K. Oh, J.-K. Chung, J.-H. Lee, W. H. Kim, C. W. Kim, B. Y. Cho, and
J.-G. Park
VT-2016
Development and Characterization of a Cell Line From a Blastula Stage Rainbow Trout Embryo
Niels C. Bols, University of Waterloo, J. L. Brubacher, K. Fujiki, B. Dixon, P. Collodi, M. P. Lamb, and
L. E. J. Lee
VT-2017
Proteomic Analysis of In Vitro Erythroid Differentiation
Jiri Petrak, Institute of Hematology and Blood Transfusion, M. Babusiak, and D. Vyoral
VT-2019
Cell Growth and Cytokine Production of Human Mixed Mullerian Tumor (LN1) Cells Grown on the
International Space Station (ISS)
Dianne K. Hammond, Wyle Life Sciences, Jeanne Becker, T. F. Elliott, K. Holubec, T. L. Baker, and
J. E. Love
Moderator:
Gertrude C. Buehring, University of California - Berkeley
2:15 pm – 3:00 pm
Vertebrate/Toxicology Interactive Poster Session..............Pacific Concourse
VT-2012
Effects of Military Jet Fuel (JP5) on the Cell Proliferation of Testicular Mouse Cell Line (TM3)
Kate Lynn Ledbetter, Texas Southern University, and James W. DuMond, Jr.
VT-2013
Suppression of Two-stage Cell Transformation by Electrolyzed Reduced Water/Platinum
Nanocolloids
Ryuhei Nishikawa, Kyushu University, K. Teruya, Y. Katakura, K. Otsubo, S. Morisawa, and S.
Shirahata
VT-2014
Suppression of Invasion of Cancer Cells and Angiogenesis by Electrolyzed Reduced Water
Ye Jun, Kyushu University, K. Teruya, Y. Katakura, K. Otsubo, S. Morisawa, and S. Shirahata
VT-2022
Perturbation of Leydig Cell Proliferation by Cadmium
Michele Walker-Brown, Texas Southern University, and James W. DuMond, Jr.
RNA INTERFERENCE:
A NEW TOOL IN GENE DISCOVERY AND GENE THERAPY
Conveners:
Harold N. Trick, Kansas State University
Guy Smagghe, Ghent University
Michael J. Fay, Midwestern University
3:00 pm – 5:00 pm
State-of-the-Art Symposium..........Grand Ballroom B
RNA interference, or RNAi, is an innate cellular process activated when a double–stranded RNA (dsRNA) molecule enters
the cell, causing the degradation of not only the invading dsRNA molecule, but also single-stranded RNAs of identical
sequences, including endogenous mRNSs. This phenomenon is apparently widespread in eukaryotes, ranging from
trypansome to human, from Neurospora to rice, and has sparked great interest from both fundamental and applied
perspectives. Currently, RNAi is being evaluated for both functional genomic analyses and for its potential in highly
specific gene-silencing therapeutics.
3:00
Introduction (H. N. Trick, G. Smagghe, and M. J. Fay)
3:15
SA-1
RNA Interference in Invertebrates
Jozef Vanden Broeck, University of Leuven
3:45
SA-2
Development of siRNA-based Therapeutics
Barry Polisky, Sirna Therapeutics
4:15
SA-3
Viral Suppression of RNA Silencing in Plants
Vicki Bowman Vance, University of South Carolina