SATURDAY, MAY 22
7:00 am – 7:00 pm
Registration..................Market Street Foyer
8:00 am – 12:00 pm
SIVB BOARD OF DIRECTORS MEETING.............................
Board Room A
11TH INTERNATIONAL
CONFERENCE ON INVERTEBRATE CELL AND TISSUE CULTURE
DONORS:
BIOPROCESSING™ JOURNAL
BIOSOURCE
BD BIOSCIENCES DISCOVERY LABWARE
PROMEGA CORPORATION
VWR SCIENTIFIC PRODUCTS
Chair: Amy A. Wang, GlaxoSmithKline
Co-Chair: Guy Smagghe, Ghent University
Invertebrate Program Committee:
Cynthia Goodman, USDA, ARS, BCIRL
Robert R. Granados, Boyce Thompson Institute for Plant Research
Raziel S. Hakim, Howard University
J. Denry Sato, Mount Desert Biological Laboratory
Dwight Lynn, USDA
Marcia Loeb, USDA
Jun Mitsuhashi, Tokyo University Agriculture
Karl Maramorosch, Rutgers University
K. J. Magaratha Vally, Texas A&M University
KEYNOTE SYMPOSIUM
AND OPENING SESSION
MOLECULAR ENGINEERING AND BIOLOGY OF INVERTEBRATE CELL CULTURES: A TRIBUTE
TO DR. THOMAS GRACE AND PROFESSOR SHANGYIN GAO
Conveners:
Robert R. Granados, Boyce Thompson Institute
Karl Maramorosch, Rutgers University
Amy Wang, GlaxoSmithKline
8:30 am – 5:30 pm
Invertebrate Symposium................Seacliff A & B
In this symposium we are paying tribute to Dr. T. Grace and Prof. Shangyin Gao (Z-Y.Gaw) to recognize their specific
tangible achievements in the field of invertebrate cell culture. The exceptional and unique achievements of these two
pioneers have resulted in the rapid development and important applications of insect cell culture during the past four
decades. Modern invertebrate cell culture got started independently by the two scientists. Prof. Shangyin Gao in Wuhan,
China in 1958 and Tom Grace in Canberra, Australia in 1962. The two have never met and they were unaware of each
other's work, but they shared outstanding talents as creative inventors. They have made invaluable contributions to
furthering the progress of the field and provided guidelines for us to follow in the footsteps of these in vitro biotechnology
pioneers. The breakthrough achieved by Grace and Gao has influenced virtually all subsequent research dealing with
insect cell culture. Therefore, we are paying tribute to these pioneers, recognizing that without their contributions no
achievement in modern molecular invertebrate cell culture would have been possible. Several biotechnological advances
have driven the remarkable growth and application of insect cell culture research during the past two decades. The
emergence of the baculovirus-insect cell culture system resulted from intensive and elegant studies on the molecular
biology of baculoviruses and the development of novel insect virus-cell culture systems. The use of in vitro expression
systems have not only become important tools for basic research around the world, but represent a widely used
technology for the commercialization of products for use in agriculture and human health. The speakers will represent
some of the leading authorities in areas relating to cell culture systems and they represent the diversity of research from
around the world.
8:30
Welcome and Announcements
Amy Wang, GlaxoSmithKline
8:40
Introduction
I-1
Seminal Research Contributions by T. Grace and S. Gao
Karl Maramorosch, Rutgers University
9:00
Presentation of Historical 1963 Film: Insect Tissue Culture
T. Grace, CSIRO Entomologist (ret.), Canberra, Australia
9:30
I-3
Invertebrate Cell Culture Applications in China
Zhihong Hu, Wuhan Institute of Virology
10:00
I-2
Invertebrate Cell Culture Biology and Novel Cell Lines
Robert R. Granados, Boyce Thompson Institute
10:35
I-5
Apoptosis Regulation in Cultured Insect Cells: Facing Death on a Hair Trigger
Rollie Clem, Kansas State University
11:10
I-6
Role of the Major Envelope Protein (GP64) of Baculoviruses in Viral Entry and Exit from Cultured
Cells
Gary Blissard, Boyce Thompson Institute
11:45
Break for Lunch
LIFETIME ACHIEVEMENT AWARD PRESENTATION
Convener:
Amy Wang, GlaxoSmithKline
12:30
Introduction (K. Maramorosch)
Lifetime Achievement Award Recipient: Thomas Grace
12:00 pm – 1:30 pm
Luncheon Honoring Dr. Thomas Grace
.............................
Seacliff C & D
1:30
I-7
Transgenic Insect Cell Lines That Support Production of Humanized Glycoprotiens by Baculovirus
Expression Vectors
Donald Jarvis, University of Wyoming
2:05
I-8
Stable Transformation of Insect Cells with Densovirus Vectors and Expression of Foreign Proteins
Max Bergoin, University of Montpellier II
2:40
I-4
Scale-up and Optimizing the In Vitro Growth of Insect Cells for Production of Recombinant Proteins
and Viral Pesticides
Spiros N. Agathos, University of Louvain
3:15 pm – 3:35 pm
Coffee Break
...........................Seacliff A & B
3:35
I-9
Molecular Biology and Genomics of Shrimp Viruses and Their In Vitro Culture
Just Vlak, Wageningen University
4:10
I-10
Invertebrate Cell Cultures for Commercial Pharmaceutical Drug Discovery
Steven H. Harwood, Invitrogen Life Technologies
4:45
I-11
Baculovirus Technology for Mammalian Cell Gene Delivery
Patrick Condreay, GlaxoSmithKline Discovery Research
5:20
I-12
Molecular Engineering and Biology of Invertebrate Cell Cultures: Summary and Conclusions
Dwight E. Lynn, USDA/ARS/BARC
USING CELL-BASED
MODEL SYSTEMS IN ASSAYS AND BASIC RESEARCH: A WORKSHOP IN
PRACTICAL METHODS
SPONSOR:
MATTEK CORPORATION
Special thanks to the following organizations that have donated their resources and time to this workshop: MatTek
Corporation, Cellz Direct, Institute for In Vitro Sciences, Neural Stem Inc., Astarte Biologics LLC, and Zen Bio.
Convener:
Jeffrey Janus, Pacgen Cellco, LLC
8:30 am – 5:00 pm
Education Workshop.............
Hospitality Room
This is a rare and valuable opportunity to learn directly from experienced experts in the use of cell-based models, with a
focus on the use of Human Cells. Attendees will be taught practical and scientific knowledge concerning culture
methodologies and the features and benefits of these models. Specific emphasis will be placed on allowing attendees to
ask questions of the speakers and participate in discussions on subjects concerning culture techniques, dosing, endpoint
selection, data analysis, and specifics concerning individual cell types. Handouts and posters providing highlights of cell-
based model examples will be available.
8:30
Cell-based Bioassays in Multi-well Plates: Practical Approaches to Assay Design and Execution
John W. Harbell, Institute for In Vitro Sciences, Inc.
9:30
Selecting Relevant Drug Concentrations and Human Cell Types for In Vitro Testing for Both Toxicity
and Efficacy
Eugene Elmore, University of California, Irvine
10:30 am – 10:45 am
Coffee Break
Hospitality Room
10:45
The Use of Hepatocytes in Drug Development Assays
Geraldine A. Hamilton, CellzDirect
11:45 am – 12:45 pm
Lunch on your own
12:45
The Use of a Stable Human Neural Stem Cell Line in In Vitro Assays for Drug Development
Karl Johe, NeuralStem, Inc.
1:45
The In Vitro Study of Cellular Immunology, Part 1
Anne Lodge, Astarte Biologics LLC
2:45 pm – 3:00 pm
Coffee Break
......................Hospitality Room
3:00
The In Vitro Study of Cellular Immunology, Part 2
Anne Lodge, Astarte Biologics LLC
4:00
Primary Human Cells in Cell-based Assays: Studies with Human Adipocytes
Dawn Franklin, Zen-Bio
FUNDING FUNDAMENTALS:
HOW TO GET THE GRANT YOU WANT
DONORS:
TEMPLE INLAND FOREST PRODUCTS
SIVB PLANT BIOTECHNOLOGY SECTION
CAISSON LABS
PHYTOTECHNOLOGY LABORATORIES INC.
TECHNIKOS RESEARCH ASSOCIATES
DR. MICHAEL HORN
ARBORGEN
Conveners:
Carol M. Stiff, Kitchen Culture Kits
Judi Heitz, Chula Vista High School
1:00 pm – 5:00 pm
Education Workshop
...........................
Garden B
Learn what the granting agencies are looking for; how to determine if your idea is fundable by a particular agency; the
main reasons for rejection; and how to avoid common pitfalls. Our panel of experts includes representatives from granting
agencies and seasoned grant writers who will give short presentations and then open the floor for discussion. Attendance
is free for SIVB meeting attendees, and will benefit both seasoned and new members, graduate students, postdocs, and
more.
1:00
Introduction (C. M. Stiff and J. Heitz)
Overview of USDA Competitive Grant Programs and Tips for Success
Mary Purcell-Miramontes, USDA/CSREES/NRI
Funding Opportunities at the National Science Foundation
Eve Ida Barak, National Science Foundation
Both Sides Examined: Notes from a Former PI and Former NSF Program Director
V. Celeste Carter, Foothill College
The Key Elements of a Proposal
Kristen Hershbell, City College of San Francisco
Winning the Paperwork Battle – Accountability After Getting the Grant
Judi Heitz, Chula Vista High School
Open Discussion/Coffee Break
6:00 pm – 7:00 pm
EDUCATION SPECIAL POSTER SESSION ..........Pacific Concourse Foyer
7:00 pm – 9:00 pm
2004 WORLD CONGRESS OPENING RECEPTION
.......Pacific Concourse
7:00 pm – 9:00 pm
STREETS OF SAN FRANCISCO
SILENT AUCTION KICKOFF
...............................Pacific Concourse
7:00 pm – 9:00 pm
Exhibits and Posters
..............Pacific Concourse
EDUCATING THE BIOTECHNOLOGY
WORKFORCE: CURRENT PROGRAMS, FUTURE
DIRECTIONS
Moderator:
Elizabeth J. Roemer, State University of New York at Stony Brook
7:30 pm – 9:00 pm
Education Interactive Poster Session
............Pacific Concourse Foyer
E-2000
Bio-Link: Educating the Biotechnology Workforce at Community and Technical Colleges
Elaine A. Johnson, City College of San Francisco
E-2001
Professional Education and Career Options for Today’s Biological Scientists
Lindy A. Brigham, The University of Arizona
E-2002
Internships: A Central Role in the Master of Biotechnology Program, a Professional Science Master’s
David Bieber, San Jose State University, and A. Christopher Brinegar
E-2003
Biotechnology Management – A New Professional Masters Degree Program
Steven A. McCommas, Southern Illinois University Edwardsville
E-2011
Community Colleges Biotechnology Manufacturing Training Programs: A Proposal
Patrick McKay, Genentech, Inc., P. Lloyd, M. Hill, and H. Stern
E-3000
An Approach to Biotechnology in the High School: Modeling DNA, RNA, and Proteins
Carol Harrison, Booker T. Washington High School, and M. Egnin
7:30 pm – 8:30 pm
All Poster Authors will be present
...............................Pacific Concourse