MONDAY, MAY 24
7:00 am – 6:00 pm
Registration..................Market Street Foyer
CONTAMINANTS IN PLANT
TISSUE CULTURE:
NEW WAYS OF ATTACKING AN OLD PROBLEM
Conveners:
Michael E. Kane, University of Florida
Valerie C. Pence, Cincinnati Zoo and Botanical Garden
Barbara M. Reed, USDA Agricultural Research Service
8:00 am – 10:00 am
Plant Symposium..................Grand Ballroom B
Bacterial and fungal contaminants are a persistent problem for commercial as well as research laboratories.
Contaminants not only inhibit growth, overgrow, or kill cultures, but also undetected organisms can affect the results of
experimental studies or cause problems for cultures transferred to soil. This symposium will provide an overview of
various antimicrobial chemicals and their use in tissue culture, new methods for detection of microorganisms in cultures,
and a range of strategies for starting and maintaining clean cultures.
8:00
Introduction (M. E. Kane, V. C. Pence, and B. M. Reed)
8:15
P-7
Physical and Chemical Means of Controlling Contamination in Plant Cell Cultures
Kenneth C. Torres, PhytoTechnology Laboratories
8:45
P-8
Review of Current Plant Indexing Techniques
Chet Sutula, Agdia, Inc.
9:15
P-9
Strategies for Producing and Maintaining Clean Cultures
Barbara M. Reed, USDA-ARS National Clonal Germplasm Repository
Moderator:
John Vogel, USDA
8:00 am – 10:00 am
Plant Contributed Paper Session......Grand Ballroom C
8:00
P-1000
Development of the SDI (Site-Direct-Integration) System for Gene Replacement
Hiroyasu Ebinuma, Nippon Paper Industries Co., LTD., K. Nanto, and K. Yamada-Watanabe
8:15
P-1001
Exploring Nicotiana Germplasm for PMP-oriented Breeding: Regenerability and Transformability of
Nicotiana Species
Baochun Li, University of Kentucky
8:30
P-1002
Construction and Testing of Centromere-based Plant Mini-chromosomes
Helge Zieler, Chromatin Inc., Jennifer Mach, Jim Jin, Gary Rudgers, and Daphne Preuss
8:45
P-1003
Genetic Transformation of Tass Fescue and Lolium temulentum
Yaxin Ge, The Samuel Roberts Noble Foundation, and Zeng-Yu Wang
9:00
P-1004
Production of Fertile Transgenic Rice and Wheat Plants via Mature Seed Electroporation
Takashi Hagio, National Institute of Agrobiological Sciences, Yutaka Tabei, Shin’ichiro Kamachi,
Sugihiro Ando, Taiichi Ogawa, and Yoshiki Habu
9:15
P-1005
Agrobacterium-mediated Transformation of the Model Grass Brachypodium distachyon
John P. Vogel, USDA, and Oymon Leong
9:30
P-1006
Transgene Expression in Site-specific Integrant Rice Lines
Magnolia Ariza-Nieto, University of Arkansas, and Vibha Srivastava
9:45
P-1007
To Dwarf Fruit Trees–Expression of the Arabidopsis gai Gene in Apple Can Strongly Reduce the
Plant Height
Li-Hua Zhu, Swedish University of Agricultural Sciences, X. Y. Li, M. Nyquist, and M. Welander
RNA INTERFERENCE
WORKSHOP
Convener:
Janis Demetrulias, Technikos Research Associates
Michael J. Fay, Midwestern University
8:00 am – 10:00 am
Animal Workshop........................Seacliff C & D
RNA interference (RNAi) describes the phenomenon in which double stranded RNA (dsRNA) promotes the
posttranscriptional destruction of homologous mRNA. The resulting silencing or knockdown in the expression of an
individual gene represents a unique in vitro model for investigating gene function. However, RNAi is more than a helpful
tool for gene function analysis. Small RNAs are involved in chromatin regulation, gene expression regulation during
development, and are of importance in the intrinsic cellular defense mechanism against invading viruses. Science
magazine considered RNAi as one of the 2002 breakthroughs of the year. This workshop will introduce many of the
issues encountered on the road to utilizing this new technology and will cover such issues as target sequence selection,
validating knockdown, characteristics of different reagents, and various applications of RNAi technology.
8:00
Introduction (J. Demetrulias and M. J. Fay)
8:15
W-1
RNA Interference in Mammalian Cells, Principles, and Applications
John J. Rossi, Beck Research Institute of the City of Hope
8:45
W-2
RNAi for Neurodegenerative Disease Therapy
Beverly L. Davidson, The University of Iowa
Convener:
Gordana V. Vunjak-Novakovic, Massachusetts Institute of Technology
8:00 am – 10:00 am
Animal Symposium
......................Seacliff A & B
Tissue engineering can potentially address tissue and organ failure by implantation of biological substitutes of native
tissues. Engineered tissues can also serve as physiologically relevant 3-dimensional models for controlled studies of
tissue development, remodeling, and cell responses to genetic end environmental stimuli. The clinical and scientific
relevance of tissue engineering critically depends on our ability to direct cells to form specialized tissues that closely
mimic native physiology and can provide functional grafts for implantation. This session will address some of the critical
scientific and clinical aspects of tissue engineering. The focus is on (a) tissue engineering of functional arteries, (b)
establishment of human muscle stem cells and their use for neural tissue engineering, and (c) state of the art of
orthopaedic tissue engineering.
8:00
Introduction (G. V. Vunjak-Novakovic)
8:10
A-5
Biomimetic Approaches to Engineered Arteries
Laura Niklason, Duke University
8:45
A-6
Tissue Engineering of Functional Cardiac Muscle
Gordana V. Vunjak-Novakovic, Massachusetts Institute of Technology
9:20
A-7
Autologous Chondrocyte Transplantation (Carticel®): Lessons Learned and Future Challenges
John McPherson, Genzyme Corporation
10:00 am – 10:30 am
Coffee Break
....................Pacific Concourse
10:00 am – 3:00 pm
Exhibits and Posters
..............Pacific Concourse
STEM CELLS: FUNDAMENTAL
ASPECTS AND THERAPEUTIC APPROACHES
Convener:
Gordana V. Vunjak-Novakovic, Massachusetts Institute of Technology
10:30 am – 12:30 pm
State-of-the-Art Symposium..........Grand Ballroom B
Pluripotent stem cells have been cultured from human, animal and plant tissues, and have shown the ability to give rise to
the variety of differentiated cell types. Studies of stem cells can have an enormous impact on the advance of our basic
understanding of cell biology as well as the development of new treatment options. In recent years, stem cell research is
becoming increasingly important both for animal and for plant cell biology. This has motivated our effort to convene this
“state of the art” session that reviews the recent research on human, animal and plant stem cells. The focus is on (a)
progenitor/stem cells isolated from adult tissues, (b) immortalized stem cells derived from human keratinocytes, and (c)
signaling systems that control the fate of plant stem cells.
10:30
Introduction (G.V. Vunjak-Novakovic)
10:40
SA-4
Osteogenic Properties of Human Myogenic Progenitor Cells
Karen Hirshi, Baylor College of Medicine
11:15
SA-5
Stem Cell Lineage of Human Regenerative Epithelia, Such as Skin, Esophagus, and Uterine Cervix
Shigeru Yasumoto, Kanagawa Cancer Center Research Institute
11:50
SA-6
Molecular Control of Stem Cell Activity in Plants
Jennifer Fletcher, USDA/University of California - Berkeley
CELL CULTURE SYSTEMS
FOR PRODUCTION OF PHAR-MACEUTICALS
Convener:
Paul J. Price, GIBCO Invitrogen
David W. Jayme, GIBCO Invitrogen
1:30 pm – 3:00 pm
Animal Symposium
......................Seacliff A & B
The capability of cultured animal cells to support high titer virus production and to synthesize and secrete proteins with
functional post-translational modifications has facilitated the biopharmaceutical production industry. The quantity and
quality of product yield is influenced by the exogenous environment, requiring unique optimization of culture and
expression conditions for each cell type and target product. Optimization may include the bioreactor type, nutrient feeding
strategy, environmental controls, and methods for product harvest and waste removal. This mini-symposium will invite
thought leaders from three major Biotech companies to present their experiences in maximizing bioreactor yield for the
manufacture of recombinant proteins, monoclonal antibodies, and vaccines.
1:30
Introduction (P. J. Price and D. W. Jayme)
1:40
A-8
Improving NS0 Fed-batch Culture Productivity
John Burky, Protein Design Labs, Inc.
2:05
A-9
Large Scale Perfusion Process Development: Platform Technologies and Automation
Chun Zhang, Bayer Biological Products
2:30
A-10
Process Development Challenges for Virus Vaccines
J. Michael Berry, MedImmune Vaccines, Inc.
1:30 pm – 2:30pm
Odd Poster Authors will be present
...............................Pacific Concourse
INVERTEBRATE INTERACTIVE
POSTERS
Moderator:
Raziel S. Hakim, Howard University
1:30 pm – 3:00 pm
Invertebrate Interactive Poster Session
.............................Pacific Concourse
I-2000
Ex Vivo Utilization of Caterpillar Midgut to Study Kinetics and Toxicity of Insect Growth Regulators
Guy Smagghe, Ghent University, K. Van Loocke, N. De Regge, H. Wouters, and J.-P. Ottoy
I-2001
Optimization of Feeding Strategies for Heterologous Protein Production in Cultures Using the Insect
Cell-Baculovirus System
Spiros N. Agathos, Catholic University of Louvain, J.-C. Drugmand, J.-F. Michiels, and Y.-J.
Schneider
I-2003
Does Apoptosis Play a Role in the Growth and Metamorphosis of the Lepidopteran Midgut?
Raziel S. Hakim, Howard University
I-2004
An Assay System for Determining the Toxicity of Flavonoids Against Insect Cells In Vitro
J. Larry Gringorten, Canadian Forest Service, M. Abou-Zaid, and G. Caputo
ALTERED PLANT ARCHITECTURE
AND CELLULAR CHEMISTRY
Moderator:
Nancy A. Reichert, Mississippi State University
Kimberly A. Rayford, St. Louis Science Center
2:00 pm – 3:00 pm
Plant Interactive Poster Session
...............................Pacific Concourse
P-2005
Expression and Molecular Analysis of bas1 in Oryza sativa ssp. Japonica cv. Nipponabare for the
Development of Dwarfism in Rice
A. Neill, University of Rhode Island, Chip Longo, H. Duong, J. West, B. Lebeau, L. Thompson, M.
Neff, J. Chandlee, H. Luo, and A. Kausch
P-2006
Genetic Manipulation of Auxin and Ethylene to Alter the Growth and Development of Populus
Joo Young Kim, University of Tennessee, Zong Ming Cheng, and Yi Li
P-2007
Biocontainment of Genetically Modified Grasses for the Control of Transgene Escape Using Male
Sterility
Chip Longo, University of Rhode Island, Q. Luo, K. Nelson, H. Luo, and A. Kausch
P-2008
Increasing Methionine Levels in Alfalfa by Co-Expressing Genes for Cystathionine Gamma Synthase,
a Key Enzyme in Methionine
Suman Bagga, New Mexico State University, Jamie Ross, Carol Potenza, Nina Klypin, Tom Leustek,
and Champa Sengupta-Gopalan
P-2009
Understanding Monolignol Biosynthesis in Medicago by Down-regulation of P-Coumarate 3-
Hydroxylase
Lisa A. Jackson, The Samuel Roberts Noble Foundation, M. S. Srinivasa Reddy, Gail Shadle, Fang
Chen, and Richard Dixon
ADVANCED TECHNOLOGIES
FOR MONOCLONAL ANTIBODY PRODUCTION
Convener:
Shinji Iijima, Nagoya University
3:00 pm –5:00 pm
Animal Symposium
......................Seacliff C & D
3:00
Introduction (S. Iijima)
3:10
A-11
Production of Human Monoclonal Antibody by In Vitro Immunization
Sanetaka Shirahata, Kyushu University
3:30
A-12
Production of Antibody by Transgenic Chicken
Shinji Iijima, Nagoya University
3:50
A-13
Antibody Selection and Production System Using a Mammalian Cell Line
Teruyuki Nagamune, The University of Tokyo
4:10
A-14
Engineering Host Cells to Maximize Effector Function of Produced Antibodies by Controlling the
Oligosaccharide Structure
Mitsuo Satoh, Kyowa Hakko Kogyo Co., Ltd.
4:30
A-15
Production of Human Antibodies in TransChromo Animals
Haruhiko Tsumura, Kirin Brewery Co., Ltd.
ALTERNATIVE SELECTION
SYSTEMS
Convener:
Allan Wenck, BASF Plant Sciences
Prakash Lakshmanan, Bureau of Sugar Experiment Stations
3:00 pm – 5:00 pm
Plant Symposium..................Grand Ballroom B
Distinguishing stably transformed cells from a background of non-transgenic cells is a major challenge in the production of
transgenic plants. Due to its relatively high selection efficiency, genes conferring herbicide and antibiotic resistance are
routinely used for transgenic plant production. However, in the recent past, consumer organizations, environmentalists,
and even some regulatory agencies have raised concerns over the use of such markers. Although there is no scientific
basis for these concerns, development of alternative markers that are viewed as environmentally benign would
undoubtedly enhance public acceptance of transgenic crops. Currently, a number of genes (e.g., Betaine aldehyde
dehydrogenase, Isopentenyl transferase, Sweet pepper ferridoxin-like protein, mannose isomerase, glutamate-1-
semialdehyde aminotransferase, etc.) have been successfully tested to develop alternative selection systems in many
plant species. This area of research will be reviewed in this symposium, with more emphasis given to the more recently
developed selection markers.
3:00
Introduction (A. Wenck and P. Lakshmanan)
3:10
P-10
Arabitol Dehydrogenase as a Selectable Marker for Plants
Peter R. LaFayette, The University of Georgia
3:35
P-11
Chloroplast Genetic Engineering for Improved Agronomic Traits and Molecular Farming, Using
Various Selection Systems
Henry Daniell, University of Central Florida
4:00
P-12
PGA37, A Gene Encoding MYB-like DNA Binding Protein, Promotes Embryogenesis in Arabidopsis
Qi-Wen Niu, Rockefeller University
4:25
P-13
A Sweet-pepper Ferrodoxin-like Protein Promises Substantial Equivalence to Its Conventional
Counterpart as a Selection Marker in Plant Genetic Engineering
Ming-Tsair Chan, Academia Sinica
Moderator:
Kim C. O’Connor, Tulane University
3:00 pm – 4:30 pm
Joint Vertebrate/Toxicology Contributed Paper Session.................
Seacliff B
3:00
VT-1000
Characterization of Neuroblastoma Organoid Growth in a Low-shear, Microgravity Rotary Bioreactor
and Comparison to In Vivo Tumors
Robert Asbury Redden, Children’s Hospital of Philadelphia, and E. J. Doolin
3:15
VT-1001
Role of Protein Kinase C Isoforms in the Epithelial to Mesenchymal Transformation of Keratinocytes
and Endothelial Cells Isolated from Human Skin Squamous Cell Carcinomas
Sirosh Bokhari, Stanford University School of Medicine, S. G. Paturi, H. B. Gladstone, and Marvin A.
Karasek
3:30
VT-1002
Monte Carlo Simulation of Prostate Cancer Cell Aggregation into Multicellular Spheroids
Kim C. O’Connor, Tulane University, Hong Song, Daniel J. Lacks, Richard M. Enmon, and Shamik
K. Jain
3:45
VT-1003
The Role of Phosphatidylcholine PLC in the Inhibition of Gap
Junction Communication, Activation of MAPK, and the Release of Arachidonic Acid by Specific
Isomers of Methylated Anthracenes
Brad Luther Upham, Michigan State University, Patti K. Tithof, and James E. Trosko
4:00
VT-1005
Expression of Cullin-4A mRNA in Breast Epithelial Cells, Breast Cancer Cell Lines, and Matched
Normal Versus Tumor Tissues
Michael J. Fay, Midwestern University, George A. Karathanasis, and David M. Shope
RESTORATION OF
ENDANGERED TREE SPECIES
Conveners:
William A. Powell, SUNY College of Environmental Science and Forestry
Katrina Gause, ArborGen LLC
3:00 pm –5:00 pm
Plant Symposium..................Grand Ballroom C
The movement of plants around the world has had several unintended and sometimes devastating consequences to
forest species. In North America alone, diseases such as white pine blister rust, chestnut blight, beech scale complex,
Dutch elm disease, butternut canker, dogwood anthracnose, and, more recently, sudden oak death have caused a
significant loss of trees in our forests and urban settings. Even with efforts to stem the movement of exotic diseases and
insects, it is likely that new transfers of diseases and pests will continue in to the future. In order to restore species from
past and ongoing epidemics, researchers are beginning to apply biotechnological techniques to forest species. Because
forest species are not as well studied as agricultural crops, most are in natural or minimally managed settings, and they
are long-lived species, there are many unique challenges in applying biotechnology to this field. In this session, we will
explore the uses of genetic engineering in restoration of American chestnut and elm species, the use of hypoviruses to
control chestnut blight, and future strategies for dealing with newly emerging diseases such as sudden oak death.
3:00
Introduction (W. A. Powell and K. Gause)
3:10
P-14
Using Biotechnology in the Battle with Dutch Elm Disease
Kevan Gartland, University of Abertay Dundee
3:35
P-15
Heritage Embraces Innovation in Chestnut Restoration
William A. Powell, State University of New York at Syracuse
4:00
P-16
Prospects of Utilizing Hypoviruses for Chestnut Blight Biological Control
William L. MacDonald, West Virginia University
4:25
P-17
Modern Molecular Approaches to Studying and Controlling Sudden Oak Death
David Rizzo, University of California at Davis
STEM CELLS IN AQUATIC
ORGANISMS
Convener:
David W. Barnes, Mount Desert Island Biological Laboratories
3:00 pm – 5:00 pm
Animal Symposium
............................
Seacliff A
The unique aspects of aquatic model systems have provided seminal insights into basic mechanisms of embryonic
development and tissue regeneration. Currently, aquatic models continue to make contributions to our understanding of
stem cell biology. Embryonal stem cell cultures have been developed from fish and are also being studied in simpler
models such as sponges, sea urchins, and mollusks. Primordial germ cell manipulation has shown early success in fish
as well, and zebrafish, elasmobranchs (sharks, rays, and skates), and other fish models are being used to great
advantage in studies of hematopoietic, myogenic, nephrogenic, and neurogenic stem cells. Additionally, primitive aquatic
models including ascidians, Aplysia, and arthropods such as shrimp, crayfish, and lobsters are a source of basic
information on neural stem cells. Shrimp and simpler organisms such as the urochordate ascidian B. schlosseri have
given insights into aspects of immunological and self-recognition. Speakers will present recent work using some of these
aquatic stem cell models in determining the basic cell biology of development and cell type-specific renewal.
3:00
Introduction (D. W. Barnes)
3:10
A-16
Germ-line Competent Zebrafish ES Cell Cultures
Paul Collodi, Purdue University
3:30
A-17
Stem Cell Biology of the Colonial Protochorate, Botryllus schlosseri
Anthony W. De Tomaso, Stanford University School of Medicine
3:50
A-18
A Genetic Screen for Mutations Affecting Organogenesis and Fin Regeneration, and Large-scale
Analysis of the Genes Involved in Fin Regeneration of the Medaka
Akira Kudo, Tokyo Institute of Technology
4:10
A-19
Cel Cultures from Marine Elasmobranchs (Sharks, Skates) and Sea Urchins
David W. Barnes, Mount Desert Island Biological Laboratories
4:30
A-36
The Manzanar Project: Down to Stems and Seeds Again
Gordon H. Sato, The Manzanar Project